Experiment: 20 mM D,L-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metC and SO2192 are separated by 125 nucleotides SO2192 and SO2193 are separated by 128 nucleotides
SO2191: metC - cystathionine beta-lyase (NCBI ptt file), at 2,302,113 to 2,303,312
metC
SO2192: SO2192 - sensor histidine kinase (NCBI ptt file), at 2,303,438 to 2,305,501
SO2192
SO2193: SO2193 - DNA-binding response regulator (NCBI ptt file), at 2,305,630 to 2,306,322
SO2193
Position (kb)
2303
2304
2305
2306 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2302.463 kb on + strand, within metC at 2302.535 kb on + strand, within metC at 2302.543 kb on - strand, within metC at 2302.543 kb on - strand, within metC at 2302.587 kb on + strand, within metC at 2302.606 kb on - strand, within metC at 2302.637 kb on - strand, within metC at 2302.686 kb on - strand, within metC at 2302.750 kb on + strand, within metC at 2302.750 kb on + strand, within metC at 2302.823 kb on - strand, within metC at 2302.990 kb on + strand, within metC at 2303.069 kb on - strand, within metC at 2303.316 kb on + strand at 2303.329 kb on - strand at 2303.540 kb on + strand at 2303.697 kb on - strand, within SO2192 at 2303.824 kb on + strand, within SO2192 at 2303.824 kb on + strand, within SO2192 at 2303.902 kb on + strand, within SO2192 at 2303.910 kb on - strand, within SO2192 at 2304.077 kb on + strand, within SO2192 at 2304.297 kb on - strand, within SO2192 at 2304.348 kb on + strand, within SO2192 at 2304.458 kb on - strand, within SO2192 at 2304.675 kb on - strand, within SO2192 at 2304.749 kb on + strand, within SO2192 at 2304.788 kb on + strand, within SO2192 at 2304.941 kb on + strand, within SO2192 at 2304.946 kb on + strand, within SO2192 at 2304.954 kb on - strand, within SO2192 at 2304.954 kb on - strand, within SO2192 at 2304.998 kb on + strand, within SO2192 at 2305.021 kb on + strand, within SO2192 at 2305.194 kb on + strand, within SO2192 at 2305.202 kb on - strand, within SO2192 at 2305.212 kb on - strand, within SO2192 at 2305.230 kb on - strand, within SO2192 at 2305.237 kb on - strand, within SO2192 at 2305.356 kb on + strand at 2305.382 kb on - strand at 2305.453 kb on - strand at 2305.524 kb on + strand at 2305.558 kb on - strand at 2305.819 kb on + strand, within SO2193 at 2305.827 kb on - strand, within SO2193 at 2305.827 kb on - strand, within SO2193 at 2306.107 kb on - strand, within SO2193 at 2306.120 kb on + strand, within SO2193 at 2306.241 kb on - strand, within SO2193 at 2306.241 kb on - strand, within SO2193 at 2306.241 kb on - strand, within SO2193 at 2306.357 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM D,L-Lactate remove 2,302,463 + metC SO2191 0.29 -3.0 2,302,535 + metC SO2191 0.35 -3.4 2,302,543 - metC SO2191 0.36 -2.4 2,302,543 - metC SO2191 0.36 -4.9 2,302,587 + metC SO2191 0.40 -2.8 2,302,606 - metC SO2191 0.41 -2.4 2,302,637 - metC SO2191 0.44 -3.0 2,302,686 - metC SO2191 0.48 -1.4 2,302,750 + metC SO2191 0.53 -1.4 2,302,750 + metC SO2191 0.53 -4.4 2,302,823 - metC SO2191 0.59 -3.0 2,302,990 + metC SO2191 0.73 -2.8 2,303,069 - metC SO2191 0.80 -3.0 2,303,316 + -2.0 2,303,329 - -0.4 2,303,540 + +0.3 2,303,697 - SO2192 0.13 -0.7 2,303,824 + SO2192 0.19 +0.2 2,303,824 + SO2192 0.19 +0.0 2,303,902 + SO2192 0.22 +0.7 2,303,910 - SO2192 0.23 -0.9 2,304,077 + SO2192 0.31 +1.0 2,304,297 - SO2192 0.42 -0.4 2,304,348 + SO2192 0.44 -1.3 2,304,458 - SO2192 0.49 -0.1 2,304,675 - SO2192 0.60 +1.4 2,304,749 + SO2192 0.64 +0.4 2,304,788 + SO2192 0.65 -1.0 2,304,941 + SO2192 0.73 -0.4 2,304,946 + SO2192 0.73 -0.5 2,304,954 - SO2192 0.73 +2.9 2,304,954 - SO2192 0.73 +1.0 2,304,998 + SO2192 0.76 +1.3 2,305,021 + SO2192 0.77 -0.9 2,305,194 + SO2192 0.85 -0.3 2,305,202 - SO2192 0.85 +0.1 2,305,212 - SO2192 0.86 -1.0 2,305,230 - SO2192 0.87 -0.6 2,305,237 - SO2192 0.87 -2.0 2,305,356 + -1.1 2,305,382 - -0.1 2,305,453 - +0.2 2,305,524 + -0.1 2,305,558 - +0.4 2,305,819 + SO2193 0.27 +0.1 2,305,827 - SO2193 0.28 -0.9 2,305,827 - SO2193 0.28 +0.0 2,306,107 - SO2193 0.69 +0.1 2,306,120 + SO2193 0.71 +1.5 2,306,241 - SO2193 0.88 -0.7 2,306,241 - SO2193 0.88 -0.0 2,306,241 - SO2193 0.88 -0.5 2,306,357 - -0.4
Or see this region's nucleotide sequence