Experiment: 20 mM D,L-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0526 and SO0527 are separated by 43 nucleotides SO0527 and SO0528 are separated by 26 nucleotides SO0528 and trpI are separated by 59 nucleotides
SO0526: SO0526 - acetyltransferase, GNAT family (NCBI ptt file), at 550,003 to 550,476
SO0526
SO0527: SO0527 - conserved hypothetical protein (NCBI ptt file), at 550,520 to 550,846
SO0527
SO0528: SO0528 - conserved hypothetical protein (NCBI ptt file), at 550,873 to 551,760
SO0528
SO0529: trpI - trpba operon transcriptional activator (NCBI ptt file), at 551,820 to 552,704
trpI
Position (kb)
550
551
552 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 549.895 kb on + strand at 549.949 kb on + strand at 549.959 kb on + strand at 549.967 kb on - strand at 549.981 kb on - strand at 550.108 kb on + strand, within SO0526 at 550.113 kb on + strand, within SO0526 at 550.116 kb on - strand, within SO0526 at 550.121 kb on - strand, within SO0526 at 550.121 kb on - strand, within SO0526 at 550.121 kb on - strand, within SO0526 at 550.238 kb on - strand, within SO0526 at 550.243 kb on - strand, within SO0526 at 550.263 kb on + strand, within SO0526 at 550.301 kb on + strand, within SO0526 at 550.309 kb on - strand, within SO0526 at 550.423 kb on - strand, within SO0526 at 550.423 kb on - strand, within SO0526 at 550.423 kb on - strand, within SO0526 at 550.430 kb on - strand at 550.456 kb on - strand at 550.461 kb on + strand at 550.664 kb on - strand, within SO0527 at 550.737 kb on + strand, within SO0527 at 550.745 kb on - strand, within SO0527 at 550.933 kb on - strand at 550.941 kb on - strand at 551.030 kb on - strand, within SO0528 at 551.106 kb on + strand, within SO0528 at 551.107 kb on + strand, within SO0528 at 551.175 kb on + strand, within SO0528 at 551.183 kb on - strand, within SO0528 at 551.249 kb on + strand, within SO0528 at 551.259 kb on + strand, within SO0528 at 551.286 kb on - strand, within SO0528 at 551.395 kb on + strand, within SO0528 at 551.395 kb on + strand, within SO0528 at 551.504 kb on + strand, within SO0528 at 551.504 kb on + strand, within SO0528 at 551.504 kb on + strand, within SO0528 at 551.512 kb on - strand, within SO0528 at 551.522 kb on - strand, within SO0528 at 551.568 kb on - strand, within SO0528 at 551.597 kb on - strand, within SO0528 at 551.602 kb on - strand, within SO0528 at 551.659 kb on + strand, within SO0528 at 551.670 kb on + strand, within SO0528 at 551.670 kb on + strand, within SO0528 at 551.678 kb on - strand at 551.711 kb on - strand at 551.725 kb on + strand at 551.731 kb on - strand at 551.763 kb on + strand at 551.783 kb on + strand at 551.845 kb on + strand at 551.853 kb on - strand at 551.862 kb on + strand at 551.873 kb on + strand at 551.873 kb on + strand at 551.926 kb on + strand, within trpI at 552.110 kb on - strand, within trpI at 552.151 kb on - strand, within trpI at 552.151 kb on - strand, within trpI at 552.243 kb on - strand, within trpI at 552.251 kb on - strand, within trpI at 552.329 kb on - strand, within trpI at 552.350 kb on + strand, within trpI at 552.351 kb on + strand, within trpI at 552.389 kb on + strand, within trpI at 552.397 kb on - strand, within trpI at 552.397 kb on - strand, within trpI at 552.410 kb on + strand, within trpI at 552.410 kb on + strand, within trpI at 552.436 kb on - strand, within trpI at 552.461 kb on + strand, within trpI at 552.469 kb on - strand, within trpI at 552.469 kb on - strand, within trpI at 552.482 kb on - strand, within trpI at 552.483 kb on + strand, within trpI at 552.491 kb on - strand, within trpI at 552.497 kb on - strand, within trpI
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM D,L-Lactate remove 549,895 + -0.1 549,949 + +1.1 549,959 + -1.9 549,967 - -0.6 549,981 - +0.2 550,108 + SO0526 0.22 +0.1 550,113 + SO0526 0.23 +0.4 550,116 - SO0526 0.24 -0.2 550,121 - SO0526 0.25 +0.1 550,121 - SO0526 0.25 -0.1 550,121 - SO0526 0.25 -2.9 550,238 - SO0526 0.50 +0.6 550,243 - SO0526 0.51 +0.1 550,263 + SO0526 0.55 +0.4 550,301 + SO0526 0.63 -0.9 550,309 - SO0526 0.65 +0.9 550,423 - SO0526 0.89 +0.1 550,423 - SO0526 0.89 +0.8 550,423 - SO0526 0.89 -0.3 550,430 - -0.4 550,456 - -0.6 550,461 + +1.5 550,664 - SO0527 0.44 +0.7 550,737 + SO0527 0.66 +0.3 550,745 - SO0527 0.69 +0.5 550,933 - +0.4 550,941 - +1.4 551,030 - SO0528 0.18 +0.5 551,106 + SO0528 0.26 +0.3 551,107 + SO0528 0.26 +0.2 551,175 + SO0528 0.34 -0.3 551,183 - SO0528 0.35 +0.0 551,249 + SO0528 0.42 +1.1 551,259 + SO0528 0.43 -0.3 551,286 - SO0528 0.47 -0.5 551,395 + SO0528 0.59 -0.8 551,395 + SO0528 0.59 -0.4 551,504 + SO0528 0.71 -1.6 551,504 + SO0528 0.71 -0.9 551,504 + SO0528 0.71 -0.1 551,512 - SO0528 0.72 -0.6 551,522 - SO0528 0.73 -0.4 551,568 - SO0528 0.78 +0.4 551,597 - SO0528 0.82 +0.2 551,602 - SO0528 0.82 +0.6 551,659 + SO0528 0.89 +0.3 551,670 + SO0528 0.90 -0.5 551,670 + SO0528 0.90 +0.5 551,678 - +1.0 551,711 - -0.1 551,725 + +0.1 551,731 - +0.1 551,763 + -0.1 551,783 + -1.2 551,845 + +0.1 551,853 - +0.3 551,862 + +0.6 551,873 + -0.2 551,873 + -1.2 551,926 + trpI SO0529 0.12 +0.9 552,110 - trpI SO0529 0.33 +0.6 552,151 - trpI SO0529 0.37 +0.1 552,151 - trpI SO0529 0.37 +0.8 552,243 - trpI SO0529 0.48 -0.0 552,251 - trpI SO0529 0.49 +2.1 552,329 - trpI SO0529 0.58 -0.6 552,350 + trpI SO0529 0.60 +0.7 552,351 + trpI SO0529 0.60 +0.2 552,389 + trpI SO0529 0.64 -0.6 552,397 - trpI SO0529 0.65 -0.6 552,397 - trpI SO0529 0.65 +2.0 552,410 + trpI SO0529 0.67 +1.1 552,410 + trpI SO0529 0.67 -1.1 552,436 - trpI SO0529 0.70 -0.5 552,461 + trpI SO0529 0.72 -0.9 552,469 - trpI SO0529 0.73 +1.1 552,469 - trpI SO0529 0.73 -0.9 552,482 - trpI SO0529 0.75 +0.5 552,483 + trpI SO0529 0.75 -0.1 552,491 - trpI SO0529 0.76 -0.5 552,497 - trpI SO0529 0.76 +0.9
Or see this region's nucleotide sequence