Experiment: 20 mM D,L-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0204 and SO0205 are separated by 283 nucleotides SO0205 and trmA are separated by 9 nucleotides trmA and murI are separated by 55 nucleotides murI and SO0208 are separated by 6 nucleotides
SO0204: SO0204 - hypothetical protein (NCBI ptt file), at 212,225 to 212,884
SO0204
SO0205: SO0205 - hypothetical protein (NCBI ptt file), at 213,168 to 213,560
SO0205
SO0206: trmA - tRNA (uracil-5-)-methyltransferase (NCBI ptt file), at 213,570 to 214,670
trmA
SO0207: murI - glutamate racemase (NCBI ptt file), at 214,726 to 215,634
murI
SO0208: SO0208 - RNA-binding protein (NCBI ptt file), at 215,641 to 216,105
SO0208
Position (kb) 213
214
215 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 212.580 kb on - strand, within SO0204 at 212.635 kb on - strand, within SO0204 at 212.656 kb on + strand, within SO0204 at 212.705 kb on + strand, within SO0204 at 212.713 kb on - strand, within SO0204 at 212.724 kb on - strand, within SO0204 at 212.747 kb on - strand, within SO0204 at 212.791 kb on + strand, within SO0204 at 212.791 kb on + strand, within SO0204 at 212.791 kb on + strand, within SO0204 at 212.793 kb on + strand, within SO0204 at 212.799 kb on - strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.810 kb on + strand, within SO0204 at 212.817 kb on + strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.818 kb on - strand, within SO0204 at 212.825 kb on + strand at 212.825 kb on + strand at 212.825 kb on + strand at 212.825 kb on + strand at 212.825 kb on + strand at 212.825 kb on + strand at 212.825 kb on + strand at 212.833 kb on - strand at 212.833 kb on - strand at 212.833 kb on - strand at 212.833 kb on - strand at 212.833 kb on - strand at 212.833 kb on - strand at 212.833 kb on - strand at 212.833 kb on - strand at 212.835 kb on + strand at 212.835 kb on + strand at 212.835 kb on + strand at 212.843 kb on - strand at 212.843 kb on - strand at 212.843 kb on - strand at 212.843 kb on - strand at 212.843 kb on - strand at 212.843 kb on - strand at 212.843 kb on - strand at 212.843 kb on - strand at 212.843 kb on - strand at 212.844 kb on + strand at 212.851 kb on + strand at 212.852 kb on - strand at 212.939 kb on + strand at 212.996 kb on - strand at 213.006 kb on + strand at 213.034 kb on + strand at 213.039 kb on - strand at 213.067 kb on + strand at 213.067 kb on + strand at 213.067 kb on + strand at 213.075 kb on - strand at 213.075 kb on - strand at 213.143 kb on - strand at 213.190 kb on + strand at 213.197 kb on - strand at 213.229 kb on + strand, within SO0205 at 213.254 kb on - strand, within SO0205 at 213.278 kb on - strand, within SO0205 at 213.302 kb on + strand, within SO0205 at 213.325 kb on + strand, within SO0205 at 213.333 kb on - strand, within SO0205 at 213.343 kb on - strand, within SO0205 at 213.349 kb on + strand, within SO0205 at 213.349 kb on + strand, within SO0205 at 213.410 kb on + strand, within SO0205 at 213.468 kb on + strand, within SO0205 at 213.468 kb on + strand, within SO0205 at 213.468 kb on + strand, within SO0205 at 213.468 kb on + strand, within SO0205 at 213.468 kb on + strand, within SO0205 at 213.473 kb on + strand, within SO0205 at 213.476 kb on - strand, within SO0205 at 213.492 kb on - strand, within SO0205 at 213.569 kb on - strand at 213.588 kb on - strand at 213.613 kb on + strand at 213.621 kb on - strand at 213.621 kb on - strand at 213.635 kb on + strand at 213.666 kb on - strand at 213.683 kb on + strand, within trmA at 213.691 kb on - strand, within trmA at 213.705 kb on - strand, within trmA at 213.741 kb on + strand, within trmA at 213.749 kb on + strand, within trmA at 213.749 kb on - strand, within trmA at 213.756 kb on + strand, within trmA at 213.756 kb on + strand, within trmA at 213.766 kb on + strand, within trmA at 213.769 kb on + strand, within trmA at 213.830 kb on + strand, within trmA at 213.919 kb on + strand, within trmA at 213.937 kb on - strand, within trmA at 213.937 kb on - strand, within trmA at 213.951 kb on + strand, within trmA at 213.954 kb on - strand, within trmA at 213.956 kb on + strand, within trmA at 213.959 kb on - strand, within trmA at 213.964 kb on - strand, within trmA at 214.023 kb on - strand, within trmA at 214.045 kb on - strand, within trmA at 214.090 kb on + strand, within trmA at 214.101 kb on + strand, within trmA at 214.114 kb on - strand, within trmA at 214.258 kb on + strand, within trmA at 214.292 kb on + strand, within trmA at 214.292 kb on + strand, within trmA at 214.300 kb on - strand, within trmA at 214.381 kb on + strand, within trmA at 214.389 kb on - strand, within trmA at 214.518 kb on - strand, within trmA at 214.537 kb on + strand, within trmA at 214.565 kb on + strand at 214.571 kb on - strand at 214.573 kb on - strand at 214.621 kb on + strand at 214.629 kb on - strand at 214.687 kb on + strand at 214.695 kb on - strand at 214.721 kb on + strand at 214.733 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM D,L-Lactate remove 212,580 - SO0204 0.54 +0.8 212,635 - SO0204 0.62 -1.1 212,656 + SO0204 0.65 -1.0 212,705 + SO0204 0.73 -0.4 212,713 - SO0204 0.74 +0.7 212,724 - SO0204 0.76 -0.2 212,747 - SO0204 0.79 -1.2 212,791 + SO0204 0.86 +0.6 212,791 + SO0204 0.86 +0.0 212,791 + SO0204 0.86 +0.6 212,793 + SO0204 0.86 -0.2 212,799 - SO0204 0.87 +0.2 212,810 + SO0204 0.89 -0.8 212,810 + SO0204 0.89 +0.3 212,810 + SO0204 0.89 +1.0 212,810 + SO0204 0.89 -0.5 212,810 + SO0204 0.89 +0.0 212,810 + SO0204 0.89 -0.3 212,810 + SO0204 0.89 +0.8 212,810 + SO0204 0.89 +0.2 212,810 + SO0204 0.89 +0.2 212,810 + SO0204 0.89 -1.8 212,810 + SO0204 0.89 -0.7 212,810 + SO0204 0.89 -1.4 212,810 + SO0204 0.89 +2.5 212,810 + SO0204 0.89 +1.2 212,810 + SO0204 0.89 -0.1 212,817 + SO0204 0.90 +0.1 212,818 - SO0204 0.90 -0.5 212,818 - SO0204 0.90 +0.0 212,818 - SO0204 0.90 +0.6 212,818 - SO0204 0.90 +1.2 212,818 - SO0204 0.90 -0.2 212,818 - SO0204 0.90 +0.3 212,818 - SO0204 0.90 +0.6 212,818 - SO0204 0.90 -1.6 212,818 - SO0204 0.90 -0.7 212,818 - SO0204 0.90 +0.7 212,818 - SO0204 0.90 +0.9 212,818 - SO0204 0.90 +0.5 212,818 - SO0204 0.90 +0.2 212,818 - SO0204 0.90 -0.2 212,818 - SO0204 0.90 -1.6 212,818 - SO0204 0.90 +2.0 212,818 - SO0204 0.90 -0.4 212,818 - SO0204 0.90 -0.1 212,825 + +0.4 212,825 + -0.3 212,825 + +0.2 212,825 + +0.1 212,825 + -0.5 212,825 + +0.7 212,825 + +0.6 212,833 - +0.4 212,833 - +0.4 212,833 - +0.6 212,833 - +0.2 212,833 - +0.5 212,833 - +0.2 212,833 - +0.4 212,833 - -0.1 212,835 + +0.2 212,835 + +1.8 212,835 + +0.4 212,843 - +0.5 212,843 - +0.1 212,843 - -0.2 212,843 - -0.5 212,843 - -1.6 212,843 - +0.1 212,843 - -0.1 212,843 - +2.6 212,843 - +0.7 212,844 + -0.8 212,851 + -0.7 212,852 - +0.2 212,939 + -0.4 212,996 - +0.3 213,006 + +0.3 213,034 + +0.8 213,039 - +0.2 213,067 + -1.2 213,067 + +0.0 213,067 + -0.7 213,075 - +0.0 213,075 - -0.3 213,143 - +1.5 213,190 + -0.3 213,197 - -0.3 213,229 + SO0205 0.16 -0.9 213,254 - SO0205 0.22 -1.4 213,278 - SO0205 0.28 +0.9 213,302 + SO0205 0.34 +0.2 213,325 + SO0205 0.40 -0.6 213,333 - SO0205 0.42 +0.0 213,343 - SO0205 0.45 +0.8 213,349 + SO0205 0.46 +1.0 213,349 + SO0205 0.46 -0.2 213,410 + SO0205 0.62 +1.5 213,468 + SO0205 0.76 +0.4 213,468 + SO0205 0.76 -0.4 213,468 + SO0205 0.76 +1.2 213,468 + SO0205 0.76 +0.4 213,468 + SO0205 0.76 +0.5 213,473 + SO0205 0.78 -0.1 213,476 - SO0205 0.78 +0.5 213,492 - SO0205 0.82 -0.2 213,569 - +0.5 213,588 - -0.3 213,613 + +0.8 213,621 - -0.2 213,621 - +0.5 213,635 + +0.1 213,666 - +0.2 213,683 + trmA SO0206 0.10 +1.2 213,691 - trmA SO0206 0.11 +0.8 213,705 - trmA SO0206 0.12 +1.3 213,741 + trmA SO0206 0.16 +0.3 213,749 + trmA SO0206 0.16 +0.7 213,749 - trmA SO0206 0.16 +0.3 213,756 + trmA SO0206 0.17 +1.7 213,756 + trmA SO0206 0.17 +0.4 213,766 + trmA SO0206 0.18 -0.0 213,769 + trmA SO0206 0.18 +2.9 213,830 + trmA SO0206 0.24 +1.0 213,919 + trmA SO0206 0.32 +1.1 213,937 - trmA SO0206 0.33 +0.9 213,937 - trmA SO0206 0.33 +2.3 213,951 + trmA SO0206 0.35 -0.2 213,954 - trmA SO0206 0.35 -0.2 213,956 + trmA SO0206 0.35 +0.4 213,959 - trmA SO0206 0.35 +0.9 213,964 - trmA SO0206 0.36 -0.5 214,023 - trmA SO0206 0.41 +0.4 214,045 - trmA SO0206 0.43 +0.2 214,090 + trmA SO0206 0.47 +0.4 214,101 + trmA SO0206 0.48 -2.4 214,114 - trmA SO0206 0.49 +0.1 214,258 + trmA SO0206 0.62 +0.2 214,292 + trmA SO0206 0.66 +1.2 214,292 + trmA SO0206 0.66 +0.3 214,300 - trmA SO0206 0.66 +1.8 214,381 + trmA SO0206 0.74 -1.4 214,389 - trmA SO0206 0.74 +0.7 214,518 - trmA SO0206 0.86 +1.3 214,537 + trmA SO0206 0.88 -0.2 214,565 + +1.5 214,571 - +0.9 214,573 - +0.2 214,621 + +1.4 214,629 - +0.4 214,687 + +0.2 214,695 - +0.8 214,721 + -0.2 214,733 + +0.4
Or see this region's nucleotide sequence