Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2917 and Psyr_2918 are separated by 43 nucleotides Psyr_2918 and Psyr_2919 are separated by 122 nucleotides Psyr_2919 and Psyr_2920 are separated by 241 nucleotides
Psyr_2917: Psyr_2917 - Prokaryotic protein of unknown function DUF849, at 3,505,838 to 3,506,725
_2917
Psyr_2918: Psyr_2918 - 3-hydroxyacyl-CoA dehydrogenase, C-terminal:3-hydroxyacyl-CoA dehydrogenase, NAD-binding protein, at 3,506,769 to 3,507,725
_2918
Psyr_2919: Psyr_2919 - conserved hypothetical protein, at 3,507,848 to 3,508,324
_2919
Psyr_2920: Psyr_2920 - transcriptional regulator, GntR family, at 3,508,566 to 3,509,285
_2920
Position (kb)
3506
3507
3508 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3505.826 kb on - strand at 3506.050 kb on + strand, within Psyr_2917 at 3506.051 kb on - strand, within Psyr_2917 at 3506.051 kb on - strand, within Psyr_2917 at 3506.257 kb on + strand, within Psyr_2917 at 3506.257 kb on + strand, within Psyr_2917 at 3506.258 kb on - strand, within Psyr_2917 at 3506.258 kb on - strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.739 kb on + strand at 3506.739 kb on + strand at 3506.739 kb on + strand at 3506.739 kb on + strand at 3506.739 kb on + strand at 3506.739 kb on + strand at 3506.739 kb on + strand at 3506.740 kb on - strand at 3506.740 kb on - strand at 3506.740 kb on - strand at 3506.778 kb on - strand at 3507.122 kb on + strand, within Psyr_2918 at 3507.123 kb on - strand, within Psyr_2918 at 3507.208 kb on - strand, within Psyr_2918 at 3507.208 kb on - strand, within Psyr_2918 at 3507.208 kb on - strand, within Psyr_2918 at 3507.246 kb on - strand, within Psyr_2918 at 3507.246 kb on - strand, within Psyr_2918 at 3507.262 kb on + strand, within Psyr_2918 at 3507.263 kb on - strand, within Psyr_2918 at 3507.282 kb on + strand, within Psyr_2918 at 3507.282 kb on + strand, within Psyr_2918 at 3507.282 kb on + strand, within Psyr_2918 at 3507.282 kb on + strand, within Psyr_2918 at 3507.361 kb on + strand, within Psyr_2918 at 3507.478 kb on - strand, within Psyr_2918 at 3507.478 kb on - strand, within Psyr_2918 at 3507.555 kb on + strand, within Psyr_2918 at 3507.555 kb on + strand, within Psyr_2918 at 3507.556 kb on - strand, within Psyr_2918 at 3507.556 kb on - strand, within Psyr_2918 at 3507.556 kb on - strand, within Psyr_2918 at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.636 kb on - strand at 3507.636 kb on - strand at 3507.826 kb on + strand at 3507.826 kb on + strand at 3507.866 kb on + strand at 3507.866 kb on + strand at 3507.866 kb on + strand at 3507.866 kb on + strand at 3507.866 kb on + strand at 3507.866 kb on + strand at 3507.867 kb on - strand at 3507.867 kb on - strand at 3507.867 kb on - strand at 3507.899 kb on + strand, within Psyr_2919 at 3507.899 kb on + strand, within Psyr_2919 at 3508.040 kb on + strand, within Psyr_2919 at 3508.041 kb on - strand, within Psyr_2919 at 3508.041 kb on - strand, within Psyr_2919 at 3508.041 kb on - strand, within Psyr_2919 at 3508.121 kb on + strand, within Psyr_2919 at 3508.122 kb on - strand, within Psyr_2919 at 3508.188 kb on + strand, within Psyr_2919 at 3508.188 kb on + strand, within Psyr_2919 at 3508.188 kb on + strand, within Psyr_2919 at 3508.188 kb on + strand, within Psyr_2919 at 3508.188 kb on + strand, within Psyr_2919 at 3508.188 kb on + strand, within Psyr_2919 at 3508.188 kb on + strand, within Psyr_2919 at 3508.188 kb on + strand, within Psyr_2919 at 3508.189 kb on - strand, within Psyr_2919 at 3508.189 kb on - strand, within Psyr_2919 at 3508.189 kb on - strand, within Psyr_2919 at 3508.249 kb on + strand, within Psyr_2919 at 3508.250 kb on - strand, within Psyr_2919 at 3508.250 kb on - strand, within Psyr_2919 at 3508.251 kb on + strand, within Psyr_2919 at 3508.252 kb on - strand, within Psyr_2919 at 3508.502 kb on + strand at 3508.503 kb on - strand at 3508.503 kb on - strand at 3508.569 kb on + strand at 3508.570 kb on - strand at 3508.570 kb on - strand at 3508.577 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 3,505,826 - +0.4 3,506,050 + Psyr_2917 0.24 +1.3 3,506,051 - Psyr_2917 0.24 -0.0 3,506,051 - Psyr_2917 0.24 -0.5 3,506,257 + Psyr_2917 0.47 -0.0 3,506,257 + Psyr_2917 0.47 +0.1 3,506,258 - Psyr_2917 0.47 -0.8 3,506,258 - Psyr_2917 0.47 -0.4 3,506,261 + Psyr_2917 0.48 +0.2 3,506,261 + Psyr_2917 0.48 -0.3 3,506,261 + Psyr_2917 0.48 +0.1 3,506,261 + Psyr_2917 0.48 +0.5 3,506,261 + Psyr_2917 0.48 -0.0 3,506,261 + Psyr_2917 0.48 -0.5 3,506,261 + Psyr_2917 0.48 -0.0 3,506,261 + Psyr_2917 0.48 +2.5 3,506,261 + Psyr_2917 0.48 +1.2 3,506,261 + Psyr_2917 0.48 +0.2 3,506,262 - Psyr_2917 0.48 -0.7 3,506,262 - Psyr_2917 0.48 -1.1 3,506,262 - Psyr_2917 0.48 +0.6 3,506,262 - Psyr_2917 0.48 +1.1 3,506,262 - Psyr_2917 0.48 +0.3 3,506,262 - Psyr_2917 0.48 -0.6 3,506,262 - Psyr_2917 0.48 +0.2 3,506,262 - Psyr_2917 0.48 -0.0 3,506,739 + -0.0 3,506,739 + +2.0 3,506,739 + +0.6 3,506,739 + +1.0 3,506,739 + -0.1 3,506,739 + -0.4 3,506,739 + -1.3 3,506,740 - -0.6 3,506,740 - -0.6 3,506,740 - +0.3 3,506,778 - +1.4 3,507,122 + Psyr_2918 0.37 +1.7 3,507,123 - Psyr_2918 0.37 -0.6 3,507,208 - Psyr_2918 0.46 +0.1 3,507,208 - Psyr_2918 0.46 +1.0 3,507,208 - Psyr_2918 0.46 +1.8 3,507,246 - Psyr_2918 0.50 -0.4 3,507,246 - Psyr_2918 0.50 -0.4 3,507,262 + Psyr_2918 0.52 +1.3 3,507,263 - Psyr_2918 0.52 +0.9 3,507,282 + Psyr_2918 0.54 +1.1 3,507,282 + Psyr_2918 0.54 +1.8 3,507,282 + Psyr_2918 0.54 +0.5 3,507,282 + Psyr_2918 0.54 +2.2 3,507,361 + Psyr_2918 0.62 +0.1 3,507,478 - Psyr_2918 0.74 +0.3 3,507,478 - Psyr_2918 0.74 +0.3 3,507,555 + Psyr_2918 0.82 -0.6 3,507,555 + Psyr_2918 0.82 +0.2 3,507,556 - Psyr_2918 0.82 +0.4 3,507,556 - Psyr_2918 0.82 -0.7 3,507,556 - Psyr_2918 0.82 -0.4 3,507,635 + -1.4 3,507,635 + -0.9 3,507,635 + -2.0 3,507,635 + +0.1 3,507,635 + +1.2 3,507,635 + +1.3 3,507,635 + -0.4 3,507,636 - -1.9 3,507,636 - +0.9 3,507,826 + +2.0 3,507,826 + -1.0 3,507,866 + +0.4 3,507,866 + +0.8 3,507,866 + +2.5 3,507,866 + -0.9 3,507,866 + +1.0 3,507,866 + -0.3 3,507,867 - -1.7 3,507,867 - -0.2 3,507,867 - +0.1 3,507,899 + Psyr_2919 0.11 +1.1 3,507,899 + Psyr_2919 0.11 +0.4 3,508,040 + Psyr_2919 0.40 +3.0 3,508,041 - Psyr_2919 0.40 +1.0 3,508,041 - Psyr_2919 0.40 -0.8 3,508,041 - Psyr_2919 0.40 -0.9 3,508,121 + Psyr_2919 0.57 +0.2 3,508,122 - Psyr_2919 0.57 +0.4 3,508,188 + Psyr_2919 0.71 +0.4 3,508,188 + Psyr_2919 0.71 +0.5 3,508,188 + Psyr_2919 0.71 +0.3 3,508,188 + Psyr_2919 0.71 +3.8 3,508,188 + Psyr_2919 0.71 -0.5 3,508,188 + Psyr_2919 0.71 -0.8 3,508,188 + Psyr_2919 0.71 +1.7 3,508,188 + Psyr_2919 0.71 +0.5 3,508,189 - Psyr_2919 0.71 +2.0 3,508,189 - Psyr_2919 0.71 -0.0 3,508,189 - Psyr_2919 0.71 +2.0 3,508,249 + Psyr_2919 0.84 +0.5 3,508,250 - Psyr_2919 0.84 -0.1 3,508,250 - Psyr_2919 0.84 -0.5 3,508,251 + Psyr_2919 0.84 -0.6 3,508,252 - Psyr_2919 0.85 +0.2 3,508,502 + +1.1 3,508,503 - -0.2 3,508,503 - +0.8 3,508,569 + -1.1 3,508,570 - +0.5 3,508,570 - +2.1 3,508,577 + -3.5
Or see this region's nucleotide sequence