Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2143 and Psyr_2144 are separated by 102 nucleotides Psyr_2144 and Psyr_2145 are separated by 87 nucleotides Psyr_2145 and Psyr_2146 overlap by 4 nucleotides Psyr_2146 and Psyr_2147 are separated by 74 nucleotides
Psyr_2143: Psyr_2143 - delta1-piperideine 2-carboxylate reductase, at 2,502,154 to 2,503,185
_2143
Psyr_2144: Psyr_2144 - conserved hypothetical protein, at 2,503,288 to 2,503,485
_2144
Psyr_2145: Psyr_2145 - conserved domain protein, at 2,503,573 to 2,503,830
_2145
Psyr_2146: Psyr_2146 - methionine aminopeptidase, type I, at 2,503,827 to 2,504,618
_2146
Psyr_2147: Psyr_2147 - conserved hypothetical protein, at 2,504,693 to 2,504,929
_2147
Position (kb)
2503
2504 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2503.049 kb on + strand, within Psyr_2143 at 2503.049 kb on + strand, within Psyr_2143 at 2503.101 kb on + strand at 2503.101 kb on + strand at 2503.102 kb on - strand at 2503.102 kb on - strand at 2503.132 kb on - strand at 2503.192 kb on + strand at 2503.192 kb on + strand at 2503.193 kb on - strand at 2503.193 kb on - strand at 2503.193 kb on - strand at 2503.229 kb on - strand at 2503.297 kb on - strand at 2503.297 kb on - strand at 2503.297 kb on - strand at 2503.332 kb on + strand, within Psyr_2144 at 2503.332 kb on + strand, within Psyr_2144 at 2503.333 kb on - strand, within Psyr_2144 at 2503.333 kb on - strand, within Psyr_2144 at 2503.459 kb on + strand, within Psyr_2144 at 2503.484 kb on - strand at 2503.543 kb on - strand at 2503.711 kb on + strand, within Psyr_2145 at 2503.923 kb on + strand, within Psyr_2146 at 2503.924 kb on - strand, within Psyr_2146 at 2503.924 kb on - strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.974 kb on + strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2503.975 kb on - strand, within Psyr_2146 at 2504.028 kb on - strand, within Psyr_2146 at 2504.028 kb on - strand, within Psyr_2146 at 2504.074 kb on + strand, within Psyr_2146 at 2504.074 kb on + strand, within Psyr_2146 at 2504.074 kb on + strand, within Psyr_2146 at 2504.075 kb on - strand, within Psyr_2146 at 2504.075 kb on - strand, within Psyr_2146 at 2504.075 kb on - strand, within Psyr_2146 at 2504.118 kb on - strand, within Psyr_2146 at 2504.165 kb on + strand, within Psyr_2146 at 2504.165 kb on + strand, within Psyr_2146 at 2504.165 kb on + strand, within Psyr_2146 at 2504.165 kb on + strand, within Psyr_2146 at 2504.166 kb on - strand, within Psyr_2146 at 2504.166 kb on - strand, within Psyr_2146 at 2504.169 kb on + strand, within Psyr_2146 at 2504.169 kb on + strand, within Psyr_2146 at 2504.169 kb on + strand, within Psyr_2146 at 2504.170 kb on - strand, within Psyr_2146 at 2504.220 kb on + strand, within Psyr_2146 at 2504.220 kb on + strand, within Psyr_2146 at 2504.220 kb on + strand, within Psyr_2146 at 2504.220 kb on + strand, within Psyr_2146 at 2504.220 kb on + strand, within Psyr_2146 at 2504.220 kb on + strand, within Psyr_2146 at 2504.220 kb on + strand, within Psyr_2146 at 2504.220 kb on + strand, within Psyr_2146 at 2504.221 kb on - strand, within Psyr_2146 at 2504.221 kb on - strand, within Psyr_2146 at 2504.221 kb on - strand, within Psyr_2146 at 2504.221 kb on - strand, within Psyr_2146 at 2504.326 kb on + strand, within Psyr_2146 at 2504.326 kb on + strand, within Psyr_2146 at 2504.361 kb on + strand, within Psyr_2146 at 2504.361 kb on + strand, within Psyr_2146 at 2504.361 kb on + strand, within Psyr_2146 at 2504.362 kb on - strand, within Psyr_2146 at 2504.362 kb on - strand, within Psyr_2146 at 2504.362 kb on - strand, within Psyr_2146 at 2504.388 kb on + strand, within Psyr_2146 at 2504.388 kb on + strand, within Psyr_2146 at 2504.388 kb on + strand, within Psyr_2146 at 2504.435 kb on + strand, within Psyr_2146 at 2504.436 kb on - strand, within Psyr_2146 at 2504.540 kb on + strand at 2504.540 kb on + strand at 2504.540 kb on + strand at 2504.540 kb on + strand at 2504.541 kb on - strand at 2504.541 kb on - strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.639 kb on + strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.640 kb on - strand at 2504.641 kb on + strand at 2504.641 kb on + strand at 2504.642 kb on - strand at 2504.642 kb on - strand at 2504.783 kb on + strand, within Psyr_2147 at 2504.784 kb on - strand, within Psyr_2147 at 2504.784 kb on - strand, within Psyr_2147 at 2504.784 kb on - strand, within Psyr_2147 at 2504.785 kb on + strand, within Psyr_2147 at 2504.785 kb on + strand, within Psyr_2147 at 2504.785 kb on + strand, within Psyr_2147 at 2504.785 kb on + strand, within Psyr_2147 at 2504.785 kb on + strand, within Psyr_2147 at 2504.785 kb on + strand, within Psyr_2147 at 2504.786 kb on - strand, within Psyr_2147 at 2504.786 kb on - strand, within Psyr_2147 at 2504.786 kb on - strand, within Psyr_2147 at 2504.786 kb on - strand, within Psyr_2147 at 2504.786 kb on - strand, within Psyr_2147
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 2,503,049 + Psyr_2143 0.87 -0.9 2,503,049 + Psyr_2143 0.87 -2.6 2,503,101 + +0.5 2,503,101 + -0.7 2,503,102 - +1.1 2,503,102 - -0.9 2,503,132 - +0.6 2,503,192 + -0.1 2,503,192 + +0.4 2,503,193 - -2.3 2,503,193 - -0.4 2,503,193 - -0.9 2,503,229 - -0.1 2,503,297 - +0.4 2,503,297 - -0.6 2,503,297 - +0.0 2,503,332 + Psyr_2144 0.22 -2.4 2,503,332 + Psyr_2144 0.22 +0.8 2,503,333 - Psyr_2144 0.23 -1.2 2,503,333 - Psyr_2144 0.23 +0.2 2,503,459 + Psyr_2144 0.86 +0.9 2,503,484 - +0.4 2,503,543 - +0.5 2,503,711 + Psyr_2145 0.53 -1.0 2,503,923 + Psyr_2146 0.12 -0.2 2,503,924 - Psyr_2146 0.12 -2.1 2,503,924 - Psyr_2146 0.12 +0.6 2,503,974 + Psyr_2146 0.19 -0.7 2,503,974 + Psyr_2146 0.19 +1.2 2,503,974 + Psyr_2146 0.19 -0.1 2,503,974 + Psyr_2146 0.19 +0.4 2,503,974 + Psyr_2146 0.19 +1.4 2,503,974 + Psyr_2146 0.19 -0.1 2,503,974 + Psyr_2146 0.19 +1.3 2,503,974 + Psyr_2146 0.19 +1.9 2,503,974 + Psyr_2146 0.19 -0.7 2,503,975 - Psyr_2146 0.19 -0.6 2,503,975 - Psyr_2146 0.19 -0.4 2,503,975 - Psyr_2146 0.19 -0.0 2,503,975 - Psyr_2146 0.19 -0.4 2,503,975 - Psyr_2146 0.19 +1.1 2,503,975 - Psyr_2146 0.19 -0.1 2,503,975 - Psyr_2146 0.19 -0.4 2,504,028 - Psyr_2146 0.25 +0.0 2,504,028 - Psyr_2146 0.25 -1.9 2,504,074 + Psyr_2146 0.31 +0.9 2,504,074 + Psyr_2146 0.31 -1.1 2,504,074 + Psyr_2146 0.31 -0.2 2,504,075 - Psyr_2146 0.31 +1.2 2,504,075 - Psyr_2146 0.31 +0.5 2,504,075 - Psyr_2146 0.31 -0.1 2,504,118 - Psyr_2146 0.37 +1.0 2,504,165 + Psyr_2146 0.43 -0.6 2,504,165 + Psyr_2146 0.43 +0.9 2,504,165 + Psyr_2146 0.43 -1.4 2,504,165 + Psyr_2146 0.43 -1.1 2,504,166 - Psyr_2146 0.43 +0.5 2,504,166 - Psyr_2146 0.43 +2.5 2,504,169 + Psyr_2146 0.43 -0.4 2,504,169 + Psyr_2146 0.43 +0.1 2,504,169 + Psyr_2146 0.43 +0.1 2,504,170 - Psyr_2146 0.43 +1.6 2,504,220 + Psyr_2146 0.50 +0.0 2,504,220 + Psyr_2146 0.50 +2.9 2,504,220 + Psyr_2146 0.50 +0.1 2,504,220 + Psyr_2146 0.50 -1.2 2,504,220 + Psyr_2146 0.50 +0.2 2,504,220 + Psyr_2146 0.50 -0.8 2,504,220 + Psyr_2146 0.50 -1.4 2,504,220 + Psyr_2146 0.50 +0.8 2,504,221 - Psyr_2146 0.50 -1.6 2,504,221 - Psyr_2146 0.50 -0.8 2,504,221 - Psyr_2146 0.50 -0.4 2,504,221 - Psyr_2146 0.50 -0.4 2,504,326 + Psyr_2146 0.63 -0.9 2,504,326 + Psyr_2146 0.63 -2.6 2,504,361 + Psyr_2146 0.67 +1.9 2,504,361 + Psyr_2146 0.67 -0.8 2,504,361 + Psyr_2146 0.67 -0.1 2,504,362 - Psyr_2146 0.68 +0.3 2,504,362 - Psyr_2146 0.68 -0.4 2,504,362 - Psyr_2146 0.68 -0.8 2,504,388 + Psyr_2146 0.71 +0.0 2,504,388 + Psyr_2146 0.71 -1.4 2,504,388 + Psyr_2146 0.71 +1.6 2,504,435 + Psyr_2146 0.77 +0.7 2,504,436 - Psyr_2146 0.77 +0.6 2,504,540 + -0.6 2,504,540 + +1.6 2,504,540 + -0.7 2,504,540 + -1.0 2,504,541 - -1.1 2,504,541 - -0.9 2,504,639 + -0.1 2,504,639 + +1.2 2,504,639 + -0.2 2,504,639 + +0.7 2,504,639 + -0.4 2,504,639 + -0.2 2,504,639 + +0.4 2,504,639 + -1.3 2,504,639 + +1.5 2,504,639 + +0.9 2,504,639 + +0.9 2,504,639 + +1.0 2,504,639 + -0.2 2,504,639 + +0.7 2,504,639 + -0.2 2,504,640 - -0.0 2,504,640 - -0.7 2,504,640 - +0.3 2,504,640 - -1.2 2,504,640 - +0.6 2,504,640 - -1.4 2,504,640 - -0.2 2,504,640 - -0.0 2,504,640 - -0.3 2,504,640 - -0.5 2,504,640 - +0.3 2,504,640 - +2.5 2,504,640 - -0.0 2,504,640 - -0.6 2,504,640 - +0.6 2,504,640 - +1.1 2,504,640 - +0.9 2,504,640 - -0.6 2,504,640 - -0.1 2,504,640 - +2.5 2,504,640 - -0.3 2,504,641 + -0.4 2,504,641 + -3.4 2,504,642 - +0.2 2,504,642 - -0.2 2,504,783 + Psyr_2147 0.38 +0.5 2,504,784 - Psyr_2147 0.38 -0.1 2,504,784 - Psyr_2147 0.38 +0.0 2,504,784 - Psyr_2147 0.38 -0.5 2,504,785 + Psyr_2147 0.39 +0.2 2,504,785 + Psyr_2147 0.39 -0.6 2,504,785 + Psyr_2147 0.39 -1.2 2,504,785 + Psyr_2147 0.39 -0.0 2,504,785 + Psyr_2147 0.39 -1.4 2,504,785 + Psyr_2147 0.39 -2.6 2,504,786 - Psyr_2147 0.39 -1.3 2,504,786 - Psyr_2147 0.39 +0.2 2,504,786 - Psyr_2147 0.39 +0.8 2,504,786 - Psyr_2147 0.39 -0.1 2,504,786 - Psyr_2147 0.39 +1.2
Or see this region's nucleotide sequence