Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1406 and SMa1408 are separated by 12 nucleotides SMa1408 and SMa1409 overlap by 4 nucleotides SMa1409 and SMa1410 are separated by 112 nucleotides
SMa1406: SMa1406 - TtuD3 hydroxypyruvate reductase, at 772,165 to 773,424
SMa1406
SMa1408: SMa1408 - dehydratase, at 773,437 to 774,207
SMa1408
SMa1409: SMa1409 - acyl-CoA transferase, at 774,204 to 775,775
SMa1409
SMa1410: SMa1410 - oxidoreductase, at 775,888 to 776,721
SMa1410
Position (kb) 774
775
776 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 773.210 kb on + strand, within SMa1406 at 773.210 kb on + strand, within SMa1406 at 773.216 kb on + strand, within SMa1406 at 773.583 kb on + strand, within SMa1408 at 773.583 kb on + strand, within SMa1408 at 773.615 kb on - strand, within SMa1408 at 773.615 kb on - strand, within SMa1408 at 773.708 kb on + strand, within SMa1408 at 773.708 kb on + strand, within SMa1408 at 773.708 kb on + strand, within SMa1408 at 773.795 kb on - strand, within SMa1408 at 773.821 kb on + strand, within SMa1408 at 773.821 kb on + strand, within SMa1408 at 773.885 kb on - strand, within SMa1408 at 774.056 kb on - strand, within SMa1408 at 774.056 kb on - strand, within SMa1408 at 774.142 kb on - strand at 774.142 kb on - strand at 774.213 kb on + strand at 774.388 kb on + strand, within SMa1409 at 774.388 kb on + strand, within SMa1409 at 774.388 kb on + strand, within SMa1409 at 774.388 kb on + strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.566 kb on + strand, within SMa1409 at 774.567 kb on - strand, within SMa1409 at 774.698 kb on - strand, within SMa1409 at 774.860 kb on + strand, within SMa1409 at 774.956 kb on + strand, within SMa1409 at 774.956 kb on + strand, within SMa1409 at 774.956 kb on + strand, within SMa1409 at 775.053 kb on + strand, within SMa1409 at 775.924 kb on + strand at 775.924 kb on + strand at 775.925 kb on - strand at 775.945 kb on + strand at 775.946 kb on - strand at 776.029 kb on + strand, within SMa1410 at 776.029 kb on + strand, within SMa1410 at 776.029 kb on + strand, within SMa1410 at 776.090 kb on + strand, within SMa1410 at 776.091 kb on - strand, within SMa1410 at 776.091 kb on - strand, within SMa1410 at 776.091 kb on - strand, within SMa1410 at 776.091 kb on - strand, within SMa1410 at 776.337 kb on - strand, within SMa1410 at 776.337 kb on - strand, within SMa1410 at 776.337 kb on - strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.563 kb on - strand, within SMa1410 at 776.606 kb on + strand, within SMa1410 at 776.606 kb on + strand, within SMa1410 at 776.607 kb on - strand, within SMa1410 at 776.668 kb on + strand at 776.668 kb on + strand at 776.720 kb on - strand at 776.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 773,210 + SMa1406 0.83 -0.2 773,210 + SMa1406 0.83 +0.2 773,216 + SMa1406 0.83 -0.3 773,583 + SMa1408 0.19 +0.4 773,583 + SMa1408 0.19 -0.1 773,615 - SMa1408 0.23 -0.2 773,615 - SMa1408 0.23 -0.8 773,708 + SMa1408 0.35 -2.2 773,708 + SMa1408 0.35 -2.1 773,708 + SMa1408 0.35 +0.1 773,795 - SMa1408 0.46 -0.5 773,821 + SMa1408 0.50 +0.6 773,821 + SMa1408 0.50 +0.4 773,885 - SMa1408 0.58 +0.0 774,056 - SMa1408 0.80 +0.6 774,056 - SMa1408 0.80 -0.6 774,142 - -0.9 774,142 - +0.1 774,213 + -1.5 774,388 + SMa1409 0.12 +0.0 774,388 + SMa1409 0.12 -1.4 774,388 + SMa1409 0.12 -1.5 774,388 + SMa1409 0.12 -0.7 774,389 - SMa1409 0.12 +0.1 774,389 - SMa1409 0.12 -0.3 774,389 - SMa1409 0.12 +0.4 774,389 - SMa1409 0.12 +0.1 774,566 + SMa1409 0.23 +0.1 774,567 - SMa1409 0.23 -1.8 774,698 - SMa1409 0.31 +0.0 774,860 + SMa1409 0.42 -0.7 774,956 + SMa1409 0.48 -0.1 774,956 + SMa1409 0.48 -0.8 774,956 + SMa1409 0.48 -2.6 775,053 + SMa1409 0.54 -0.5 775,924 + +0.1 775,924 + +0.1 775,925 - +0.7 775,945 + -0.7 775,946 - -0.6 776,029 + SMa1410 0.17 -0.4 776,029 + SMa1410 0.17 +0.2 776,029 + SMa1410 0.17 +0.1 776,090 + SMa1410 0.24 +0.1 776,091 - SMa1410 0.24 -0.0 776,091 - SMa1410 0.24 -0.3 776,091 - SMa1410 0.24 -1.0 776,091 - SMa1410 0.24 +1.6 776,337 - SMa1410 0.54 -0.7 776,337 - SMa1410 0.54 -0.2 776,337 - SMa1410 0.54 -0.9 776,562 + SMa1410 0.81 -0.2 776,562 + SMa1410 0.81 -0.3 776,562 + SMa1410 0.81 -0.2 776,562 + SMa1410 0.81 -4.6 776,562 + SMa1410 0.81 +0.5 776,563 - SMa1410 0.81 +0.2 776,606 + SMa1410 0.86 -0.2 776,606 + SMa1410 0.86 -0.0 776,607 - SMa1410 0.86 -0.1 776,668 + -0.6 776,668 + -0.0 776,720 - -1.1 776,764 + -0.6
Or see this region's nucleotide sequence