Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1213

Experiment: KB with Emodin 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1211 and Psyr_1212 are separated by 6 nucleotidesPsyr_1212 and Psyr_1213 are separated by 2 nucleotidesPsyr_1213 and Psyr_1214 overlap by 4 nucleotides Psyr_1211: Psyr_1211 - type III secretion protein HrpP, at 1,368,029 to 1,368,601 _1211 Psyr_1212: Psyr_1212 - type III secretion protein HrpO, at 1,368,608 to 1,369,054 _1212 Psyr_1213: Psyr_1213 - type III secretion cytoplasmic ATPase HrcN, at 1,369,057 to 1,370,406 _1213 Psyr_1214: Psyr_1214 - type III secretion protein HrpQ, at 1,370,403 to 1,371,377 _1214 Position (kb) 1369 1370 1371Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1368.362 kb on + strand, within Psyr_1211at 1368.362 kb on + strand, within Psyr_1211at 1368.363 kb on - strand, within Psyr_1211at 1368.363 kb on - strand, within Psyr_1211at 1368.455 kb on + strand, within Psyr_1211at 1368.455 kb on + strand, within Psyr_1211at 1368.455 kb on + strand, within Psyr_1211at 1368.455 kb on + strand, within Psyr_1211at 1368.455 kb on + strand, within Psyr_1211at 1368.455 kb on + strand, within Psyr_1211at 1368.455 kb on + strand, within Psyr_1211at 1368.455 kb on + strand, within Psyr_1211at 1368.455 kb on + strand, within Psyr_1211at 1368.456 kb on - strand, within Psyr_1211at 1368.456 kb on - strand, within Psyr_1211at 1368.456 kb on - strand, within Psyr_1211at 1368.456 kb on - strand, within Psyr_1211at 1368.456 kb on - strand, within Psyr_1211at 1368.609 kb on + strandat 1368.610 kb on - strandat 1368.716 kb on + strand, within Psyr_1212at 1368.862 kb on + strand, within Psyr_1212at 1368.863 kb on - strand, within Psyr_1212at 1368.905 kb on + strand, within Psyr_1212at 1368.906 kb on - strand, within Psyr_1212at 1369.151 kb on - strandat 1369.151 kb on - strandat 1369.152 kb on + strandat 1369.206 kb on - strand, within Psyr_1213at 1369.239 kb on + strand, within Psyr_1213at 1369.239 kb on + strand, within Psyr_1213at 1369.240 kb on - strand, within Psyr_1213at 1369.241 kb on + strand, within Psyr_1213at 1369.241 kb on + strand, within Psyr_1213at 1369.241 kb on + strand, within Psyr_1213at 1369.242 kb on - strand, within Psyr_1213at 1369.242 kb on - strand, within Psyr_1213at 1369.378 kb on + strand, within Psyr_1213at 1369.378 kb on + strand, within Psyr_1213at 1369.378 kb on + strand, within Psyr_1213at 1369.379 kb on - strand, within Psyr_1213at 1369.379 kb on - strand, within Psyr_1213at 1369.450 kb on + strand, within Psyr_1213at 1369.457 kb on + strand, within Psyr_1213at 1369.457 kb on + strand, within Psyr_1213at 1369.457 kb on + strand, within Psyr_1213at 1369.523 kb on + strand, within Psyr_1213at 1369.524 kb on - strand, within Psyr_1213at 1369.524 kb on - strand, within Psyr_1213at 1369.524 kb on - strand, within Psyr_1213at 1370.086 kb on + strand, within Psyr_1213at 1370.086 kb on + strand, within Psyr_1213at 1370.086 kb on + strand, within Psyr_1213at 1370.164 kb on - strand, within Psyr_1213at 1370.351 kb on + strandat 1370.352 kb on - strandat 1370.493 kb on + strandat 1370.493 kb on + strandat 1370.494 kb on - strandat 1370.494 kb on - strandat 1370.494 kb on - strandat 1370.494 kb on - strandat 1370.494 kb on - strandat 1370.497 kb on + strandat 1370.497 kb on + strandat 1370.497 kb on + strandat 1370.497 kb on + strandat 1370.497 kb on + strandat 1370.498 kb on - strandat 1370.498 kb on - strandat 1370.498 kb on - strandat 1370.498 kb on - strandat 1370.498 kb on - strandat 1370.498 kb on - strandat 1370.498 kb on - strandat 1370.498 kb on - strandat 1370.620 kb on + strand, within Psyr_1214at 1370.844 kb on - strand, within Psyr_1214at 1371.366 kb on + strandat 1371.366 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Emodin 10 mM
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1,368,362 + Psyr_1211 0.58 +1.1
1,368,362 + Psyr_1211 0.58 -0.3
1,368,363 - Psyr_1211 0.58 +1.5
1,368,363 - Psyr_1211 0.58 +0.4
1,368,455 + Psyr_1211 0.74 +0.4
1,368,455 + Psyr_1211 0.74 +0.6
1,368,455 + Psyr_1211 0.74 +1.1
1,368,455 + Psyr_1211 0.74 +1.6
1,368,455 + Psyr_1211 0.74 +0.0
1,368,455 + Psyr_1211 0.74 +0.4
1,368,455 + Psyr_1211 0.74 +3.9
1,368,455 + Psyr_1211 0.74 -0.2
1,368,455 + Psyr_1211 0.74 +0.8
1,368,456 - Psyr_1211 0.75 -2.1
1,368,456 - Psyr_1211 0.75 -0.0
1,368,456 - Psyr_1211 0.75 -1.9
1,368,456 - Psyr_1211 0.75 -0.2
1,368,456 - Psyr_1211 0.75 +0.8
1,368,609 + +0.4
1,368,610 - -0.9
1,368,716 + Psyr_1212 0.24 -1.1
1,368,862 + Psyr_1212 0.57 -0.7
1,368,863 - Psyr_1212 0.57 +0.7
1,368,905 + Psyr_1212 0.66 +0.7
1,368,906 - Psyr_1212 0.67 +0.1
1,369,151 - -2.4
1,369,151 - -0.3
1,369,152 + +0.2
1,369,206 - Psyr_1213 0.11 +0.3
1,369,239 + Psyr_1213 0.13 +0.8
1,369,239 + Psyr_1213 0.13 +0.5
1,369,240 - Psyr_1213 0.14 -1.9
1,369,241 + Psyr_1213 0.14 -1.7
1,369,241 + Psyr_1213 0.14 -0.2
1,369,241 + Psyr_1213 0.14 +0.6
1,369,242 - Psyr_1213 0.14 +1.4
1,369,242 - Psyr_1213 0.14 -0.8
1,369,378 + Psyr_1213 0.24 -0.1
1,369,378 + Psyr_1213 0.24 -0.3
1,369,378 + Psyr_1213 0.24 -0.1
1,369,379 - Psyr_1213 0.24 -0.3
1,369,379 - Psyr_1213 0.24 +1.7
1,369,450 + Psyr_1213 0.29 +0.3
1,369,457 + Psyr_1213 0.30 +0.2
1,369,457 + Psyr_1213 0.30 -0.5
1,369,457 + Psyr_1213 0.30 -0.1
1,369,523 + Psyr_1213 0.35 +2.1
1,369,524 - Psyr_1213 0.35 +1.2
1,369,524 - Psyr_1213 0.35 +0.4
1,369,524 - Psyr_1213 0.35 -0.0
1,370,086 + Psyr_1213 0.76 -0.9
1,370,086 + Psyr_1213 0.76 -1.8
1,370,086 + Psyr_1213 0.76 +2.3
1,370,164 - Psyr_1213 0.82 +0.5
1,370,351 + +1.5
1,370,352 - +0.0
1,370,493 + +0.8
1,370,493 + +0.8
1,370,494 - +0.1
1,370,494 - +1.1
1,370,494 - +1.9
1,370,494 - -1.3
1,370,494 - +0.4
1,370,497 + -0.2
1,370,497 + +0.3
1,370,497 + +0.5
1,370,497 + -0.1
1,370,497 + -0.4
1,370,498 - +0.6
1,370,498 - -1.3
1,370,498 - +1.1
1,370,498 - +0.3
1,370,498 - +3.7
1,370,498 - +0.3
1,370,498 - -0.2
1,370,498 - +0.9
1,370,620 + Psyr_1214 0.22 -2.3
1,370,844 - Psyr_1214 0.45 -0.7
1,371,366 + +0.1
1,371,366 + -0.8

Or see this region's nucleotide sequence