Experiment: DinoMM minimal media with L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2986 and HP15_2987 are separated by 6 nucleotides HP15_2987 and HP15_2988 are separated by 13 nucleotides HP15_2988 and HP15_2989 are separated by 39 nucleotides HP15_2989 and HP15_2990 are separated by 0 nucleotides
HP15_2986: HP15_2986 - FeS assembly protein SufD, at 3,129,877 to 3,131,163
_2986
HP15_2987: HP15_2987 - cysteine desulfurase, SufS subfamily, at 3,131,170 to 3,132,423
_2987
HP15_2988: HP15_2988 - FeS cluster insertion, at 3,132,437 to 3,132,790
_2988
HP15_2989: HP15_2989 - metal-sulfur cluster biosynthetic enzyme, at 3,132,830 to 3,133,372
_2989
HP15_2990: HP15_2990 - SufE protein probably involved in Fe-S center assembly, at 3,133,373 to 3,133,843
_2990
Position (kb)
3131
3132
3133 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3131.113 kb on + strand at 3131.113 kb on + strand at 3131.113 kb on + strand at 3131.113 kb on + strand at 3131.113 kb on + strand at 3132.396 kb on + strand at 3132.876 kb on + strand at 3132.918 kb on + strand, within HP15_2989 at 3132.978 kb on - strand, within HP15_2989 at 3132.983 kb on - strand, within HP15_2989 at 3133.088 kb on + strand, within HP15_2989 at 3133.301 kb on - strand, within HP15_2989 at 3133.313 kb on - strand, within HP15_2989
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Histidine remove 3,131,113 + -0.8 3,131,113 + -2.4 3,131,113 + -0.8 3,131,113 + -0.7 3,131,113 + -0.7 3,132,396 + +0.3 3,132,876 + +0.5 3,132,918 + HP15_2989 0.16 -1.2 3,132,978 - HP15_2989 0.27 -0.1 3,132,983 - HP15_2989 0.28 -0.8 3,133,088 + HP15_2989 0.48 -1.1 3,133,301 - HP15_2989 0.87 -3.0 3,133,313 - HP15_2989 0.89 -1.4
Or see this region's nucleotide sequence