Experiment: DinoMM minimal media with L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1251 and HP15_1252 are separated by 6 nucleotides HP15_1252 and HP15_1253 are separated by 213 nucleotides HP15_1253 and HP15_1254 are separated by 16 nucleotides
HP15_1251: HP15_1251 - large component of pyruvate transporter (actP-like) (from data) , at 1,310,720 to 1,312,486
_1251
HP15_1252: HP15_1252 - small component of pyruvate transporter (TIGR03647) (from data) , at 1,312,493 to 1,312,759
_1252
HP15_1253: HP15_1253 - DNA polymerase III, epsilon subunit, at 1,312,973 to 1,313,680
_1253
HP15_1254: HP15_1254 - cyclic nucleotide-binding domain (cNMP-BD) protein, at 1,313,697 to 1,315,613
_1254
Position (kb)
1312
1313 Strain fitness (log2 ratio)
-2
-1
0
1 at 1311.936 kb on + strand, within HP15_1251 at 1311.941 kb on + strand, within HP15_1251 at 1311.949 kb on - strand, within HP15_1251 at 1311.963 kb on - strand, within HP15_1251 at 1312.019 kb on + strand, within HP15_1251 at 1312.032 kb on + strand, within HP15_1251 at 1312.068 kb on - strand, within HP15_1251 at 1312.114 kb on - strand, within HP15_1251 at 1312.383 kb on + strand at 1312.393 kb on - strand at 1312.401 kb on + strand at 1312.431 kb on + strand at 1312.581 kb on - strand, within HP15_1252 at 1312.601 kb on + strand, within HP15_1252 at 1312.750 kb on - strand at 1312.750 kb on - strand at 1312.917 kb on - strand at 1312.917 kb on - strand at 1312.917 kb on - strand at 1312.917 kb on - strand at 1312.998 kb on + strand at 1313.077 kb on + strand, within HP15_1253 at 1313.139 kb on - strand, within HP15_1253 at 1313.159 kb on + strand, within HP15_1253 at 1313.167 kb on - strand, within HP15_1253 at 1313.186 kb on - strand, within HP15_1253 at 1313.186 kb on - strand, within HP15_1253 at 1313.186 kb on - strand, within HP15_1253 at 1313.441 kb on - strand, within HP15_1253 at 1313.533 kb on - strand, within HP15_1253
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Histidine remove 1,311,936 + HP15_1251 0.69 +0.0 1,311,941 + HP15_1251 0.69 +1.0 1,311,949 - HP15_1251 0.70 -1.0 1,311,963 - HP15_1251 0.70 -0.2 1,312,019 + HP15_1251 0.74 -1.5 1,312,032 + HP15_1251 0.74 -0.1 1,312,068 - HP15_1251 0.76 -0.1 1,312,114 - HP15_1251 0.79 -0.1 1,312,383 + -0.3 1,312,393 - -0.5 1,312,401 + -0.0 1,312,431 + -0.5 1,312,581 - HP15_1252 0.33 -1.3 1,312,601 + HP15_1252 0.40 -0.1 1,312,750 - -1.3 1,312,750 - +0.6 1,312,917 - +0.2 1,312,917 - +0.1 1,312,917 - -0.8 1,312,917 - -0.2 1,312,998 + -1.8 1,313,077 + HP15_1253 0.15 -1.1 1,313,139 - HP15_1253 0.23 +0.6 1,313,159 + HP15_1253 0.26 +0.0 1,313,167 - HP15_1253 0.27 +0.7 1,313,186 - HP15_1253 0.30 -1.2 1,313,186 - HP15_1253 0.30 +0.1 1,313,186 - HP15_1253 0.30 -0.4 1,313,441 - HP15_1253 0.66 +0.1 1,313,533 - HP15_1253 0.79 +1.5
Or see this region's nucleotide sequence