Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4725 and BT4726 are separated by 21 nucleotides BT4726 and BT4727 are separated by 29 nucleotides BT4727 and BT4728 are separated by 15 nucleotides
BT4725: BT4725 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 6,202,152 to 6,203,495
BT4725
BT4726: BT4726 - glycerophosphoryl diester phosphodiesterase (NCBI ptt file), at 6,203,517 to 6,206,048
BT4726
BT4727: BT4727 - glycerophosphoryl diester phosphodiesterase (NCBI ptt file), at 6,206,078 to 6,206,983
BT4727
BT4728: BT4728 - putative hexose phosphate transport protein (NCBI ptt file), at 6,206,999 to 6,208,357
BT4728
Position (kb)
6203
6204
6205
6206
6207 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 6202.575 kb on + strand, within BT4725 at 6202.575 kb on + strand, within BT4725 at 6202.580 kb on + strand, within BT4725 at 6202.615 kb on + strand, within BT4725 at 6202.616 kb on - strand, within BT4725 at 6202.625 kb on + strand, within BT4725 at 6202.630 kb on - strand, within BT4725 at 6202.706 kb on - strand, within BT4725 at 6202.748 kb on - strand, within BT4725 at 6202.748 kb on - strand, within BT4725 at 6202.830 kb on - strand, within BT4725 at 6202.850 kb on + strand, within BT4725 at 6202.965 kb on - strand, within BT4725 at 6203.012 kb on + strand, within BT4725 at 6203.013 kb on - strand, within BT4725 at 6203.022 kb on + strand, within BT4725 at 6203.052 kb on - strand, within BT4725 at 6203.157 kb on - strand, within BT4725 at 6203.157 kb on - strand, within BT4725 at 6203.159 kb on - strand, within BT4725 at 6203.425 kb on - strand at 6203.635 kb on - strand at 6203.635 kb on - strand at 6203.635 kb on - strand at 6203.691 kb on + strand at 6203.754 kb on + strand at 6203.755 kb on - strand at 6203.772 kb on + strand, within BT4726 at 6203.912 kb on - strand, within BT4726 at 6203.912 kb on - strand, within BT4726 at 6203.952 kb on + strand, within BT4726 at 6203.952 kb on + strand, within BT4726 at 6203.953 kb on - strand, within BT4726 at 6203.953 kb on - strand, within BT4726 at 6203.973 kb on + strand, within BT4726 at 6204.068 kb on + strand, within BT4726 at 6204.069 kb on - strand, within BT4726 at 6204.069 kb on - strand, within BT4726 at 6204.072 kb on - strand, within BT4726 at 6204.072 kb on - strand, within BT4726 at 6204.089 kb on - strand, within BT4726 at 6204.103 kb on - strand, within BT4726 at 6204.103 kb on - strand, within BT4726 at 6204.237 kb on - strand, within BT4726 at 6204.274 kb on + strand, within BT4726 at 6204.275 kb on - strand, within BT4726 at 6204.275 kb on - strand, within BT4726 at 6204.275 kb on - strand, within BT4726 at 6204.276 kb on + strand, within BT4726 at 6204.276 kb on + strand, within BT4726 at 6204.277 kb on - strand, within BT4726 at 6204.277 kb on - strand, within BT4726 at 6204.277 kb on - strand, within BT4726 at 6204.277 kb on - strand, within BT4726 at 6204.278 kb on + strand, within BT4726 at 6204.278 kb on + strand, within BT4726 at 6204.297 kb on - strand, within BT4726 at 6204.320 kb on + strand, within BT4726 at 6204.321 kb on - strand, within BT4726 at 6204.363 kb on - strand, within BT4726 at 6204.401 kb on + strand, within BT4726 at 6204.401 kb on - strand, within BT4726 at 6204.491 kb on - strand, within BT4726 at 6204.491 kb on - strand, within BT4726 at 6204.562 kb on + strand, within BT4726 at 6204.888 kb on + strand, within BT4726 at 6204.897 kb on - strand, within BT4726 at 6205.062 kb on - strand, within BT4726 at 6205.494 kb on - strand, within BT4726 at 6205.494 kb on - strand, within BT4726 at 6205.497 kb on + strand, within BT4726 at 6205.543 kb on + strand, within BT4726 at 6205.644 kb on - strand, within BT4726 at 6205.695 kb on + strand, within BT4726 at 6205.695 kb on + strand, within BT4726 at 6205.695 kb on + strand, within BT4726 at 6205.716 kb on + strand, within BT4726 at 6205.717 kb on - strand, within BT4726 at 6205.746 kb on - strand, within BT4726 at 6205.785 kb on - strand, within BT4726 at 6205.785 kb on - strand, within BT4726 at 6205.792 kb on - strand, within BT4726 at 6205.815 kb on - strand at 6205.838 kb on + strand at 6205.931 kb on + strand at 6206.012 kb on - strand at 6206.052 kb on + strand at 6206.053 kb on - strand at 6206.053 kb on - strand at 6206.057 kb on - strand at 6206.059 kb on - strand at 6206.059 kb on - strand at 6206.062 kb on + strand at 6206.062 kb on + strand at 6206.092 kb on - strand at 6206.184 kb on + strand, within BT4727 at 6206.184 kb on + strand, within BT4727 at 6206.185 kb on - strand, within BT4727 at 6206.394 kb on - strand, within BT4727 at 6206.434 kb on - strand, within BT4727 at 6206.499 kb on - strand, within BT4727 at 6206.590 kb on - strand, within BT4727 at 6206.627 kb on + strand, within BT4727 at 6206.627 kb on + strand, within BT4727 at 6206.628 kb on - strand, within BT4727 at 6206.628 kb on - strand, within BT4727 at 6206.628 kb on - strand, within BT4727 at 6206.629 kb on + strand, within BT4727 at 6206.635 kb on + strand, within BT4727 at 6206.635 kb on + strand, within BT4727 at 6206.703 kb on + strand, within BT4727 at 6206.718 kb on + strand at 6206.795 kb on - strand, within BT4727 at 6206.795 kb on - strand, within BT4727 at 6206.912 kb on - strand at 6206.918 kb on + strand at 6206.953 kb on + strand at 6206.976 kb on - strand at 6206.976 kb on - strand at 6206.987 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 6,202,575 + BT4725 0.31 +1.1 6,202,575 + BT4725 0.31 -2.4 6,202,580 + BT4725 0.32 -0.9 6,202,615 + BT4725 0.34 -2.1 6,202,616 - BT4725 0.35 -2.1 6,202,625 + BT4725 0.35 -1.1 6,202,630 - BT4725 0.36 +2.2 6,202,706 - BT4725 0.41 -1.7 6,202,748 - BT4725 0.44 -1.1 6,202,748 - BT4725 0.44 -1.1 6,202,830 - BT4725 0.50 +1.6 6,202,850 + BT4725 0.52 -0.1 6,202,965 - BT4725 0.60 -2.9 6,203,012 + BT4725 0.64 +0.9 6,203,013 - BT4725 0.64 +3.0 6,203,022 + BT4725 0.65 +0.3 6,203,052 - BT4725 0.67 -0.1 6,203,157 - BT4725 0.75 -0.4 6,203,157 - BT4725 0.75 -0.1 6,203,159 - BT4725 0.75 +1.2 6,203,425 - +2.2 6,203,635 - -1.5 6,203,635 - +0.5 6,203,635 - -3.3 6,203,691 + -2.2 6,203,754 + +2.0 6,203,755 - -0.1 6,203,772 + BT4726 0.10 -1.1 6,203,912 - BT4726 0.16 -1.1 6,203,912 - BT4726 0.16 -1.3 6,203,952 + BT4726 0.17 -0.1 6,203,952 + BT4726 0.17 +2.7 6,203,953 - BT4726 0.17 -1.4 6,203,953 - BT4726 0.17 -1.1 6,203,973 + BT4726 0.18 -0.9 6,204,068 + BT4726 0.22 -3.3 6,204,069 - BT4726 0.22 -2.7 6,204,069 - BT4726 0.22 -1.7 6,204,072 - BT4726 0.22 -0.9 6,204,072 - BT4726 0.22 -1.3 6,204,089 - BT4726 0.23 -0.1 6,204,103 - BT4726 0.23 -1.7 6,204,103 - BT4726 0.23 -1.1 6,204,237 - BT4726 0.28 -4.0 6,204,274 + BT4726 0.30 -4.4 6,204,275 - BT4726 0.30 -0.7 6,204,275 - BT4726 0.30 -1.2 6,204,275 - BT4726 0.30 +0.5 6,204,276 + BT4726 0.30 -1.1 6,204,276 + BT4726 0.30 -0.1 6,204,277 - BT4726 0.30 +2.5 6,204,277 - BT4726 0.30 -1.7 6,204,277 - BT4726 0.30 -2.9 6,204,277 - BT4726 0.30 -2.6 6,204,278 + BT4726 0.30 -1.7 6,204,278 + BT4726 0.30 -3.6 6,204,297 - BT4726 0.31 -2.0 6,204,320 + BT4726 0.32 -2.1 6,204,321 - BT4726 0.32 -1.6 6,204,363 - BT4726 0.33 -1.7 6,204,401 + BT4726 0.35 -2.7 6,204,401 - BT4726 0.35 -2.4 6,204,491 - BT4726 0.38 +0.3 6,204,491 - BT4726 0.38 -1.9 6,204,562 + BT4726 0.41 -1.7 6,204,888 + BT4726 0.54 -2.1 6,204,897 - BT4726 0.55 -4.0 6,205,062 - BT4726 0.61 -3.5 6,205,494 - BT4726 0.78 -4.1 6,205,494 - BT4726 0.78 -1.7 6,205,497 + BT4726 0.78 -1.7 6,205,543 + BT4726 0.80 -0.5 6,205,644 - BT4726 0.84 -3.1 6,205,695 + BT4726 0.86 -0.1 6,205,695 + BT4726 0.86 +1.9 6,205,695 + BT4726 0.86 +1.5 6,205,716 + BT4726 0.87 -2.4 6,205,717 - BT4726 0.87 -1.7 6,205,746 - BT4726 0.88 +1.0 6,205,785 - BT4726 0.90 -0.3 6,205,785 - BT4726 0.90 +1.5 6,205,792 - BT4726 0.90 -3.1 6,205,815 - -1.4 6,205,838 + -0.1 6,205,931 + -1.1 6,206,012 - +3.8 6,206,052 + -0.7 6,206,053 - +0.9 6,206,053 - +0.2 6,206,057 - +1.3 6,206,059 - -1.7 6,206,059 - -0.9 6,206,062 + -1.7 6,206,062 + +0.5 6,206,092 - -0.6 6,206,184 + BT4727 0.12 -2.6 6,206,184 + BT4727 0.12 -0.1 6,206,185 - BT4727 0.12 -0.5 6,206,394 - BT4727 0.35 -2.7 6,206,434 - BT4727 0.39 +0.7 6,206,499 - BT4727 0.46 -1.6 6,206,590 - BT4727 0.57 -1.5 6,206,627 + BT4727 0.61 -1.7 6,206,627 + BT4727 0.61 -1.6 6,206,628 - BT4727 0.61 +0.6 6,206,628 - BT4727 0.61 +3.6 6,206,628 - BT4727 0.61 -2.7 6,206,629 + BT4727 0.61 -3.0 6,206,635 + BT4727 0.61 -2.4 6,206,635 + BT4727 0.61 -0.1 6,206,703 + BT4727 0.69 +1.5 6,206,718 + -0.1 6,206,795 - BT4727 0.79 -1.1 6,206,795 - BT4727 0.79 -2.1 6,206,912 - -0.1 6,206,918 + +0.5 6,206,953 + -0.1 6,206,976 - +1.9 6,206,976 - -1.9 6,206,987 - -1.1
Or see this region's nucleotide sequence