Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4645 and BT4646 are separated by 61 nucleotides BT4646 and BT4647 are separated by 198 nucleotides BT4647 and BT4648 are separated by 4 nucleotides
BT4645: BT4645 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 6,079,264 to 6,080,805
BT4645
BT4646: BT4646 - conserved hypothetical protein (NCBI ptt file), at 6,080,867 to 6,081,043
BT4646
BT4647: BT4647 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 6,081,242 to 6,081,778
BT4647
BT4648: BT4648 - hypothetical protein (NCBI ptt file), at 6,081,783 to 6,082,142
BT4648
Position (kb)
6080
6081
6082 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6079.976 kb on - strand, within BT4645 at 6080.119 kb on - strand, within BT4645 at 6080.148 kb on - strand, within BT4645 at 6080.189 kb on + strand, within BT4645 at 6080.189 kb on + strand, within BT4645 at 6080.190 kb on - strand, within BT4645 at 6080.270 kb on + strand, within BT4645 at 6080.294 kb on + strand, within BT4645 at 6080.294 kb on + strand, within BT4645 at 6080.295 kb on - strand, within BT4645 at 6080.394 kb on - strand, within BT4645 at 6080.460 kb on - strand, within BT4645 at 6080.482 kb on - strand, within BT4645 at 6080.491 kb on + strand, within BT4645 at 6080.512 kb on + strand, within BT4645 at 6080.512 kb on + strand, within BT4645 at 6080.513 kb on - strand, within BT4645 at 6080.513 kb on - strand, within BT4645 at 6080.513 kb on - strand, within BT4645 at 6080.513 kb on - strand, within BT4645 at 6080.513 kb on - strand, within BT4645 at 6080.513 kb on - strand, within BT4645 at 6080.663 kb on + strand at 6080.674 kb on - strand at 6080.674 kb on - strand at 6080.677 kb on - strand at 6080.736 kb on - strand at 6080.861 kb on + strand at 6080.930 kb on + strand, within BT4646 at 6080.960 kb on - strand, within BT4646 at 6080.960 kb on - strand, within BT4646 at 6081.015 kb on - strand, within BT4646 at 6081.068 kb on - strand at 6081.146 kb on - strand at 6081.333 kb on - strand, within BT4647 at 6081.335 kb on - strand, within BT4647 at 6081.449 kb on - strand, within BT4647 at 6081.496 kb on + strand, within BT4647 at 6081.517 kb on - strand, within BT4647 at 6081.551 kb on - strand, within BT4647 at 6081.614 kb on - strand, within BT4647 at 6081.677 kb on + strand, within BT4647 at 6081.706 kb on + strand, within BT4647 at 6081.762 kb on - strand at 6081.766 kb on - strand at 6081.766 kb on - strand at 6081.929 kb on + strand, within BT4648
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 6,079,976 - BT4645 0.46 -2.7 6,080,119 - BT4645 0.55 +2.0 6,080,148 - BT4645 0.57 -3.3 6,080,189 + BT4645 0.60 -1.1 6,080,189 + BT4645 0.60 +2.7 6,080,190 - BT4645 0.60 -0.1 6,080,270 + BT4645 0.65 -3.6 6,080,294 + BT4645 0.67 -2.4 6,080,294 + BT4645 0.67 +0.1 6,080,295 - BT4645 0.67 +0.0 6,080,394 - BT4645 0.73 -0.5 6,080,460 - BT4645 0.78 -1.1 6,080,482 - BT4645 0.79 +0.2 6,080,491 + BT4645 0.80 -1.1 6,080,512 + BT4645 0.81 -3.3 6,080,512 + BT4645 0.81 +1.5 6,080,513 - BT4645 0.81 -0.1 6,080,513 - BT4645 0.81 -3.1 6,080,513 - BT4645 0.81 +1.6 6,080,513 - BT4645 0.81 -0.1 6,080,513 - BT4645 0.81 +2.0 6,080,513 - BT4645 0.81 -0.3 6,080,663 + +1.0 6,080,674 - -2.6 6,080,674 - +1.5 6,080,677 - -0.9 6,080,736 - +2.5 6,080,861 + -2.9 6,080,930 + BT4646 0.36 +1.7 6,080,960 - BT4646 0.53 +1.9 6,080,960 - BT4646 0.53 +3.6 6,081,015 - BT4646 0.84 -2.1 6,081,068 - -1.7 6,081,146 - +0.6 6,081,333 - BT4647 0.17 -0.9 6,081,335 - BT4647 0.17 -0.5 6,081,449 - BT4647 0.39 +1.3 6,081,496 + BT4647 0.47 +1.9 6,081,517 - BT4647 0.51 -2.9 6,081,551 - BT4647 0.58 -0.8 6,081,614 - BT4647 0.69 +2.0 6,081,677 + BT4647 0.81 -2.1 6,081,706 + BT4647 0.86 -1.1 6,081,762 - +1.0 6,081,766 - -0.1 6,081,766 - -3.3 6,081,929 + BT4648 0.41 -2.9
Or see this region's nucleotide sequence