Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4388 and BT4389 overlap by 4 nucleotides BT4389 and BT4390 are separated by 83 nucleotides BT4390 and BT4391 are separated by 15 nucleotides BT4391 and BT4392 are separated by 215 nucleotides
BT4388: BT4388 - GTP-binding protein (NCBI ptt file), at 5,781,027 to 5,782,193
BT4388
BT4389: BT4389 - conserved hypothetical protein (NCBI ptt file), at 5,782,190 to 5,783,002
BT4389
BT4390: BT4390 - conserved hypothetical protein (NCBI ptt file), at 5,783,086 to 5,783,751
BT4390
BT4391: BT4391 - putative metalloendopeptidase (NCBI ptt file), at 5,783,767 to 5,784,405
BT4391
BT4392: BT4392 - putative Tn5 transposase (NCBI ptt file), at 5,784,621 to 5,785,886
BT4392
Position (kb)
5783
5784 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 5782.226 kb on + strand at 5782.301 kb on + strand, within BT4389 at 5782.319 kb on - strand, within BT4389 at 5782.319 kb on - strand, within BT4389 at 5782.326 kb on - strand, within BT4389 at 5782.457 kb on + strand, within BT4389 at 5782.458 kb on - strand, within BT4389 at 5782.458 kb on - strand, within BT4389 at 5782.578 kb on - strand, within BT4389 at 5782.578 kb on - strand, within BT4389 at 5782.582 kb on + strand, within BT4389 at 5782.583 kb on - strand, within BT4389 at 5782.637 kb on + strand, within BT4389 at 5782.637 kb on + strand, within BT4389 at 5782.645 kb on - strand, within BT4389 at 5782.651 kb on + strand, within BT4389 at 5782.652 kb on - strand, within BT4389 at 5782.652 kb on - strand, within BT4389 at 5782.786 kb on + strand, within BT4389 at 5782.796 kb on - strand, within BT4389 at 5782.912 kb on + strand, within BT4389 at 5782.912 kb on + strand, within BT4389 at 5782.913 kb on - strand, within BT4389 at 5782.916 kb on + strand, within BT4389 at 5782.917 kb on - strand, within BT4389 at 5782.917 kb on - strand, within BT4389 at 5782.917 kb on - strand, within BT4389 at 5783.016 kb on - strand at 5783.047 kb on + strand at 5783.068 kb on - strand at 5783.086 kb on - strand at 5783.151 kb on - strand at 5783.176 kb on + strand, within BT4390 at 5783.206 kb on - strand, within BT4390 at 5783.230 kb on - strand, within BT4390 at 5783.288 kb on - strand, within BT4390 at 5783.372 kb on - strand, within BT4390 at 5783.382 kb on + strand, within BT4390 at 5783.382 kb on + strand, within BT4390 at 5783.383 kb on - strand, within BT4390 at 5783.456 kb on + strand, within BT4390 at 5783.736 kb on + strand at 5783.750 kb on - strand at 5783.764 kb on + strand at 5783.765 kb on - strand at 5783.777 kb on + strand at 5783.804 kb on + strand at 5783.804 kb on + strand at 5783.847 kb on + strand, within BT4391 at 5783.848 kb on - strand, within BT4391 at 5783.865 kb on - strand, within BT4391 at 5783.874 kb on + strand, within BT4391 at 5783.898 kb on + strand, within BT4391 at 5783.905 kb on - strand, within BT4391 at 5783.906 kb on + strand, within BT4391 at 5783.906 kb on + strand, within BT4391 at 5783.906 kb on + strand, within BT4391 at 5783.942 kb on - strand, within BT4391 at 5784.136 kb on + strand, within BT4391 at 5784.136 kb on + strand, within BT4391 at 5784.174 kb on + strand, within BT4391 at 5784.327 kb on + strand, within BT4391 at 5784.327 kb on + strand, within BT4391 at 5784.334 kb on - strand, within BT4391 at 5784.458 kb on - strand at 5784.460 kb on + strand at 5784.508 kb on + strand at 5784.508 kb on + strand at 5784.509 kb on - strand at 5784.509 kb on - strand at 5784.544 kb on - strand at 5784.565 kb on - strand at 5784.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 5,782,226 + -1.1 5,782,301 + BT4389 0.14 -2.5 5,782,319 - BT4389 0.16 +3.1 5,782,319 - BT4389 0.16 +0.7 5,782,326 - BT4389 0.17 -2.2 5,782,457 + BT4389 0.33 +0.1 5,782,458 - BT4389 0.33 -1.2 5,782,458 - BT4389 0.33 +0.5 5,782,578 - BT4389 0.48 +2.7 5,782,578 - BT4389 0.48 +0.6 5,782,582 + BT4389 0.48 +0.1 5,782,583 - BT4389 0.48 -1.6 5,782,637 + BT4389 0.55 +0.1 5,782,637 + BT4389 0.55 +0.8 5,782,645 - BT4389 0.56 -0.8 5,782,651 + BT4389 0.57 +0.4 5,782,652 - BT4389 0.57 -1.9 5,782,652 - BT4389 0.57 -1.9 5,782,786 + BT4389 0.73 -1.8 5,782,796 - BT4389 0.75 +0.1 5,782,912 + BT4389 0.89 -1.5 5,782,912 + BT4389 0.89 +0.1 5,782,913 - BT4389 0.89 +1.1 5,782,916 + BT4389 0.89 -2.9 5,782,917 - BT4389 0.89 +0.9 5,782,917 - BT4389 0.89 -1.6 5,782,917 - BT4389 0.89 +3.8 5,783,016 - -1.1 5,783,047 + +2.9 5,783,068 - -2.5 5,783,086 - -0.7 5,783,151 - -0.9 5,783,176 + BT4390 0.14 -0.2 5,783,206 - BT4390 0.18 +0.1 5,783,230 - BT4390 0.22 +1.1 5,783,288 - BT4390 0.30 +1.1 5,783,372 - BT4390 0.43 -1.7 5,783,382 + BT4390 0.44 +0.1 5,783,382 + BT4390 0.44 +1.4 5,783,383 - BT4390 0.45 -4.2 5,783,456 + BT4390 0.56 +1.7 5,783,736 + -0.6 5,783,750 - +1.7 5,783,764 + -2.2 5,783,765 - -1.4 5,783,777 + +0.3 5,783,804 + +0.1 5,783,804 + +0.4 5,783,847 + BT4391 0.13 +1.8 5,783,848 - BT4391 0.13 -0.7 5,783,865 - BT4391 0.15 +1.8 5,783,874 + BT4391 0.17 +3.0 5,783,898 + BT4391 0.21 +2.1 5,783,905 - BT4391 0.22 +0.1 5,783,906 + BT4391 0.22 -1.7 5,783,906 + BT4391 0.22 +2.1 5,783,906 + BT4391 0.22 +2.1 5,783,942 - BT4391 0.27 -0.2 5,784,136 + BT4391 0.58 -1.2 5,784,136 + BT4391 0.58 -1.8 5,784,174 + BT4391 0.64 -2.9 5,784,327 + BT4391 0.88 +2.1 5,784,327 + BT4391 0.88 -0.5 5,784,334 - BT4391 0.89 +1.4 5,784,458 - -1.3 5,784,460 + -1.4 5,784,508 + +1.5 5,784,508 + +0.2 5,784,509 - -2.5 5,784,509 - -2.2 5,784,544 - +1.1 5,784,565 - -0.4 5,784,631 - -1.2
Or see this region's nucleotide sequence