Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4325 and BT4326 are separated by 45 nucleotides BT4326 and BT4327 are separated by 7 nucleotides BT4327 and BT4328 are separated by 21 nucleotides BT4328 and BT4329 are separated by 6 nucleotides
BT4325: BT4325 - hypothetical protein (NCBI ptt file), at 5,697,911 to 5,699,116
BT4325
BT4326: BT4326 - ABC transporter ATP-binding protein (NCBI ptt file), at 5,699,162 to 5,699,815
BT4326
BT4327: BT4327 - hypothetical protein (NCBI ptt file), at 5,699,823 to 5,701,370
BT4327
BT4328: BT4328 - conserved hypothetical protein (NCBI ptt file), at 5,701,392 to 5,702,090
BT4328
BT4329: BT4329 - conserved hypothetical protein with a conserved domain (NCBI ptt file), at 5,702,097 to 5,702,693
BT4329
Position (kb)
5699
5700
5701
5702 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5698.961 kb on - strand, within BT4325 at 5699.160 kb on + strand at 5699.161 kb on - strand at 5699.161 kb on - strand at 5699.197 kb on + strand at 5699.584 kb on - strand, within BT4326 at 5699.593 kb on + strand, within BT4326 at 5699.643 kb on + strand, within BT4326 at 5699.736 kb on - strand, within BT4326 at 5699.736 kb on - strand, within BT4326 at 5699.736 kb on - strand, within BT4326 at 5699.825 kb on - strand at 5699.825 kb on - strand at 5699.839 kb on - strand at 5699.934 kb on + strand at 5699.938 kb on + strand at 5699.939 kb on - strand at 5700.015 kb on + strand, within BT4327 at 5700.048 kb on - strand, within BT4327 at 5700.152 kb on - strand, within BT4327 at 5700.152 kb on - strand, within BT4327 at 5700.152 kb on - strand, within BT4327 at 5700.152 kb on - strand, within BT4327 at 5700.203 kb on - strand, within BT4327 at 5700.203 kb on - strand, within BT4327 at 5700.444 kb on - strand, within BT4327 at 5700.521 kb on - strand, within BT4327 at 5700.602 kb on - strand, within BT4327 at 5700.712 kb on + strand, within BT4327 at 5700.713 kb on - strand, within BT4327 at 5700.939 kb on - strand, within BT4327 at 5700.939 kb on - strand, within BT4327 at 5700.939 kb on - strand, within BT4327 at 5700.939 kb on - strand, within BT4327 at 5701.070 kb on + strand, within BT4327 at 5701.071 kb on - strand, within BT4327 at 5701.072 kb on + strand, within BT4327 at 5701.072 kb on + strand, within BT4327 at 5701.073 kb on - strand, within BT4327 at 5701.248 kb on - strand at 5701.371 kb on - strand at 5701.371 kb on - strand at 5701.404 kb on + strand at 5701.461 kb on - strand at 5701.463 kb on - strand, within BT4328 at 5701.957 kb on + strand, within BT4328 at 5701.958 kb on - strand, within BT4328 at 5701.980 kb on - strand, within BT4328 at 5702.090 kb on + strand at 5702.205 kb on - strand, within BT4329
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 5,698,961 - BT4325 0.87 +2.1 5,699,160 + -2.1 5,699,161 - -2.9 5,699,161 - +0.1 5,699,197 + -2.5 5,699,584 - BT4326 0.65 -2.5 5,699,593 + BT4326 0.66 -3.5 5,699,643 + BT4326 0.74 -2.9 5,699,736 - BT4326 0.88 -1.1 5,699,736 - BT4326 0.88 -1.2 5,699,736 - BT4326 0.88 +1.1 5,699,825 - +1.7 5,699,825 - +3.1 5,699,839 - +1.4 5,699,934 + -0.9 5,699,938 + +0.1 5,699,939 - -1.1 5,700,015 + BT4327 0.12 +0.1 5,700,048 - BT4327 0.15 -1.0 5,700,152 - BT4327 0.21 -1.8 5,700,152 - BT4327 0.21 +0.4 5,700,152 - BT4327 0.21 +0.1 5,700,152 - BT4327 0.21 -0.9 5,700,203 - BT4327 0.25 +2.5 5,700,203 - BT4327 0.25 +1.1 5,700,444 - BT4327 0.40 -0.9 5,700,521 - BT4327 0.45 +2.0 5,700,602 - BT4327 0.50 +3.3 5,700,712 + BT4327 0.57 +1.7 5,700,713 - BT4327 0.57 -0.3 5,700,939 - BT4327 0.72 +3.1 5,700,939 - BT4327 0.72 +1.1 5,700,939 - BT4327 0.72 +3.5 5,700,939 - BT4327 0.72 +0.5 5,701,070 + BT4327 0.81 +1.7 5,701,071 - BT4327 0.81 -1.7 5,701,072 + BT4327 0.81 +2.5 5,701,072 + BT4327 0.81 -3.0 5,701,073 - BT4327 0.81 -2.4 5,701,248 - -0.9 5,701,371 - +2.1 5,701,371 - -0.8 5,701,404 + -0.7 5,701,461 - -1.2 5,701,463 - BT4328 0.10 +1.3 5,701,957 + BT4328 0.81 -4.2 5,701,958 - BT4328 0.81 -1.5 5,701,980 - BT4328 0.84 -2.7 5,702,090 + +1.7 5,702,205 - BT4329 0.18 -2.5
Or see this region's nucleotide sequence