Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4238 and BT4239 are separated by 10 nucleotides BT4239 and BT4240 are separated by 45 nucleotides BT4240 and BT4241 are separated by 29 nucleotides
BT4238: BT4238 - putative permease (NCBI ptt file), at 5,581,346 to 5,582,245
BT4238
BT4239: BT4239 - hypothetical protein (NCBI ptt file), at 5,582,256 to 5,582,906
BT4239
BT4240: BT4240 - conserved hypothetical protein, with a phosphotransferase enzyme family domain (NCBI ptt file), at 5,582,952 to 5,584,043
BT4240
BT4241: BT4241 - beta-galactosidase (NCBI ptt file), at 5,584,073 to 5,587,384
BT4241
Position (kb)
5582
5583
5584
5585 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 5582.198 kb on - strand at 5582.226 kb on + strand at 5582.227 kb on - strand at 5582.280 kb on - strand at 5582.280 kb on - strand at 5582.280 kb on - strand at 5582.351 kb on + strand, within BT4239 at 5582.493 kb on + strand, within BT4239 at 5582.539 kb on + strand, within BT4239 at 5582.556 kb on - strand, within BT4239 at 5582.582 kb on + strand, within BT4239 at 5582.622 kb on + strand, within BT4239 at 5582.625 kb on - strand, within BT4239 at 5582.715 kb on + strand, within BT4239 at 5582.752 kb on + strand, within BT4239 at 5582.753 kb on - strand, within BT4239 at 5582.753 kb on - strand, within BT4239 at 5582.783 kb on + strand, within BT4239 at 5582.817 kb on + strand, within BT4239 at 5582.819 kb on + strand, within BT4239 at 5582.819 kb on + strand, within BT4239 at 5582.820 kb on - strand, within BT4239 at 5582.875 kb on - strand at 5582.928 kb on + strand at 5583.000 kb on - strand at 5583.054 kb on - strand at 5583.054 kb on - strand at 5583.064 kb on + strand, within BT4240 at 5583.076 kb on + strand, within BT4240 at 5583.079 kb on + strand, within BT4240 at 5583.096 kb on - strand, within BT4240 at 5583.098 kb on - strand, within BT4240 at 5583.126 kb on - strand, within BT4240 at 5583.148 kb on + strand, within BT4240 at 5583.162 kb on + strand, within BT4240 at 5583.393 kb on + strand, within BT4240 at 5583.415 kb on - strand, within BT4240 at 5583.499 kb on + strand, within BT4240 at 5583.500 kb on - strand, within BT4240 at 5583.500 kb on - strand, within BT4240 at 5583.513 kb on + strand, within BT4240 at 5583.549 kb on + strand, within BT4240 at 5583.550 kb on - strand, within BT4240 at 5583.576 kb on - strand, within BT4240 at 5583.626 kb on + strand, within BT4240 at 5583.689 kb on - strand, within BT4240 at 5583.689 kb on - strand, within BT4240 at 5583.712 kb on - strand, within BT4240 at 5583.855 kb on - strand, within BT4240 at 5583.855 kb on - strand, within BT4240 at 5583.913 kb on + strand, within BT4240 at 5583.949 kb on + strand at 5583.963 kb on - strand at 5583.984 kb on - strand at 5584.057 kb on - strand at 5584.069 kb on - strand at 5584.069 kb on - strand at 5584.086 kb on + strand at 5584.149 kb on + strand at 5584.215 kb on - strand at 5584.296 kb on - strand at 5584.533 kb on + strand, within BT4241 at 5584.534 kb on - strand, within BT4241 at 5584.534 kb on - strand, within BT4241 at 5584.534 kb on - strand, within BT4241 at 5584.540 kb on + strand, within BT4241 at 5584.557 kb on + strand, within BT4241 at 5584.557 kb on + strand, within BT4241 at 5584.620 kb on + strand, within BT4241 at 5584.621 kb on - strand, within BT4241 at 5584.625 kb on + strand, within BT4241 at 5584.631 kb on - strand, within BT4241 at 5584.631 kb on - strand, within BT4241 at 5584.821 kb on - strand, within BT4241 at 5584.825 kb on + strand, within BT4241 at 5584.826 kb on - strand, within BT4241 at 5584.826 kb on - strand, within BT4241 at 5584.827 kb on + strand, within BT4241 at 5584.864 kb on - strand, within BT4241
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 5,582,198 - +4.1 5,582,226 + -0.9 5,582,227 - -3.3 5,582,280 - +1.8 5,582,280 - -0.8 5,582,280 - -3.0 5,582,351 + BT4239 0.15 +0.8 5,582,493 + BT4239 0.36 -2.2 5,582,539 + BT4239 0.43 +0.2 5,582,556 - BT4239 0.46 -1.8 5,582,582 + BT4239 0.50 -2.2 5,582,622 + BT4239 0.56 -1.4 5,582,625 - BT4239 0.57 +0.2 5,582,715 + BT4239 0.71 -2.1 5,582,752 + BT4239 0.76 -1.6 5,582,753 - BT4239 0.76 +0.9 5,582,753 - BT4239 0.76 -2.4 5,582,783 + BT4239 0.81 -1.1 5,582,817 + BT4239 0.86 -0.8 5,582,819 + BT4239 0.86 +2.3 5,582,819 + BT4239 0.86 -0.3 5,582,820 - BT4239 0.87 -2.4 5,582,875 - -1.8 5,582,928 + +0.4 5,583,000 - +3.8 5,583,054 - -1.3 5,583,054 - +0.2 5,583,064 + BT4240 0.10 -1.5 5,583,076 + BT4240 0.11 +1.2 5,583,079 + BT4240 0.12 +2.2 5,583,096 - BT4240 0.13 +3.0 5,583,098 - BT4240 0.13 +2.0 5,583,126 - BT4240 0.16 -1.9 5,583,148 + BT4240 0.18 -3.7 5,583,162 + BT4240 0.19 -0.0 5,583,393 + BT4240 0.40 +3.0 5,583,415 - BT4240 0.42 -2.5 5,583,499 + BT4240 0.50 +0.2 5,583,500 - BT4240 0.50 -1.7 5,583,500 - BT4240 0.50 +0.7 5,583,513 + BT4240 0.51 +1.7 5,583,549 + BT4240 0.55 -0.1 5,583,550 - BT4240 0.55 +0.3 5,583,576 - BT4240 0.57 +1.2 5,583,626 + BT4240 0.62 -1.9 5,583,689 - BT4240 0.67 +1.2 5,583,689 - BT4240 0.67 +2.8 5,583,712 - BT4240 0.70 -2.4 5,583,855 - BT4240 0.83 +0.2 5,583,855 - BT4240 0.83 +1.9 5,583,913 + BT4240 0.88 -0.8 5,583,949 + -1.4 5,583,963 - -2.0 5,583,984 - -0.2 5,584,057 - +1.4 5,584,069 - -2.3 5,584,069 - +3.0 5,584,086 + -0.2 5,584,149 + -1.1 5,584,215 - -1.1 5,584,296 - +1.5 5,584,533 + BT4241 0.14 +2.7 5,584,534 - BT4241 0.14 -1.9 5,584,534 - BT4241 0.14 +0.2 5,584,534 - BT4241 0.14 -2.4 5,584,540 + BT4241 0.14 -2.5 5,584,557 + BT4241 0.15 +0.2 5,584,557 + BT4241 0.15 -0.6 5,584,620 + BT4241 0.17 +2.2 5,584,621 - BT4241 0.17 +1.2 5,584,625 + BT4241 0.17 +2.3 5,584,631 - BT4241 0.17 +4.2 5,584,631 - BT4241 0.17 -1.8 5,584,821 - BT4241 0.23 -0.4 5,584,825 + BT4241 0.23 -2.4 5,584,826 - BT4241 0.23 -3.0 5,584,826 - BT4241 0.23 -1.0 5,584,827 + BT4241 0.23 -2.0 5,584,864 - BT4241 0.24 -0.6
Or see this region's nucleotide sequence