Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4081

Experiment: BHIS with 5-Fluorouracil 0.1 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	BHIS with 5-Fluorouracil 0.1 mM
5,321,876	+		BT4080	0.33	-2.4
5,321,877	-		BT4080	0.33	-1.7
5,321,877	-		BT4080	0.33	-2.7
5,321,909	+		BT4080	0.35	-1.9
5,321,910	-		BT4080	0.35	-2.2
5,321,922	-		BT4080	0.36	-3.6
5,321,963	+		BT4080	0.39	-3.2
5,321,964	-		BT4080	0.39	-1.4
5,322,017	-		BT4080	0.43	+0.6
5,322,035	+		BT4080	0.44	+2.5
5,322,113	+		BT4080	0.50	+1.1
5,322,130	-		BT4080	0.51	+0.1
5,322,151	-		BT4080	0.53	-0.9
5,322,162	-		BT4080	0.53	+1.1
5,322,162	-		BT4080	0.53	-0.3
5,322,207	-		BT4080	0.57	-2.3
5,322,207	-		BT4080	0.57	-2.0
5,322,284	-		BT4080	0.62	+0.1
5,322,311	-		BT4080	0.64	-2.1
5,322,376	-		BT4080	0.69	-2.0
5,322,431	+		BT4080	0.73	+0.1
5,322,440	+		BT4080	0.74	+0.7
5,322,446	+		BT4080	0.74	-1.4
5,322,534	-		BT4080	0.81	-1.9
5,322,609	-		BT4080	0.86	-2.0
5,322,636	+		BT4080	0.88	+0.1
5,322,636	+		BT4080	0.88	-1.9
5,322,637	-		BT4080	0.88	-3.0
5,322,637	-		BT4080	0.88	-1.0
5,322,645	-		BT4080	0.89	+0.1
5,322,746	-				-0.0
5,322,799	-				+1.7
5,322,843	-				-1.8
5,322,885	-				-2.4
5,322,921	+				-1.9
5,322,966	-				-1.9
5,322,967	+				+1.1
5,322,973	-				-1.1
5,322,995	+				+1.9
5,322,996	-				-1.4
5,322,996	-				-0.4
5,323,003	-				-0.7
5,323,006	-				-0.9
5,323,039	+				-0.9
5,323,120	-				-0.2
5,323,161	-		BT4081	0.11	+4.5
5,323,173	+		BT4081	0.11	+0.1
5,323,248	+		BT4081	0.13	+2.1
5,323,250	+		BT4081	0.13	-2.4
5,323,255	-		BT4081	0.14	+2.7
5,323,262	+		BT4081	0.14	-1.8
5,323,263	-		BT4081	0.14	-0.8
5,323,363	-		BT4081	0.17	-0.1
5,323,366	-		BT4081	0.17	-2.7
5,323,366	-		BT4081	0.17	-1.0
5,323,499	+		BT4081	0.22	+1.6
5,323,503	-		BT4081	0.22	-2.1
5,323,503	-		BT4081	0.22	+0.7
5,323,578	-		BT4081	0.24	+3.1
5,323,594	+		BT4081	0.25	-0.9
5,323,595	-		BT4081	0.25	-1.2
5,323,660	-		BT4081	0.27	+2.3
5,323,664	-		BT4081	0.27	-2.2
5,323,700	+		BT4081	0.28	+1.7
5,323,774	+		BT4081	0.30	-1.6
5,323,775	-		BT4081	0.30	+0.1
5,323,775	-		BT4081	0.30	+1.1
5,323,779	-		BT4081	0.31	-1.6
5,323,824	-		BT4081	0.32	+1.0
5,323,850	-		BT4081	0.33	-1.7
5,323,869	+		BT4081	0.33	-0.3
5,323,874	-		BT4081	0.34	-0.9
5,323,891	+		BT4081	0.34	-1.4
5,323,892	-		BT4081	0.34	-1.0
5,323,896	+		BT4081	0.34	-3.6
5,323,902	+		BT4081	0.34	+0.7
5,323,903	-		BT4081	0.35	+3.1
5,323,925	+		BT4081	0.35	-1.9
5,323,925	+		BT4081	0.35	+0.7
5,323,926	-		BT4081	0.35	+0.1
5,323,952	-		BT4081	0.36	+1.0
5,323,972	-		BT4081	0.37	-2.7
5,324,010	+		BT4081	0.38	-1.1
5,324,010	+		BT4081	0.38	+1.5
5,324,010	+		BT4081	0.38	-0.2
5,324,067	-		BT4081	0.40	-2.0
5,324,078	+		BT4081	0.40	-2.3
5,324,099	-		BT4081	0.41	-2.9
5,324,158	-		BT4081	0.43	-0.5
5,324,191	-		BT4081	0.44	-0.3
5,324,195	+		BT4081	0.44	-0.9
5,324,202	+		BT4081	0.44	+1.7
5,324,273	+		BT4081	0.46	+0.7
5,324,380	-		BT4081	0.50	+1.7
5,324,383	+		BT4081	0.50	-0.9
5,324,383	+		BT4081	0.50	-1.2
5,324,386	-		BT4081	0.50	-2.2
5,324,386	-		BT4081	0.50	+0.8
5,324,417	-		BT4081	0.51	+3.1
5,324,437	+		BT4081	0.52	-2.6
5,324,438	-		BT4081	0.52	-0.3
5,324,482	+		BT4081	0.53	+1.0
5,324,486	-		BT4081	0.53	+1.5
5,324,487	+		BT4081	0.53	+2.5
5,324,496	+		BT4081	0.54	-2.7
5,324,497	-		BT4081	0.54	-1.3
5,324,523	+		BT4081	0.55	+3.8
5,324,568	+		BT4081	0.56	-0.9
5,324,573	-		BT4081	0.56	-3.6
5,324,573	-		BT4081	0.56	-1.3
5,324,590	+		BT4081	0.57	-1.4
5,324,590	+		BT4081	0.57	-0.6
5,324,706	-		BT4081	0.60	+1.9
5,324,718	-		BT4081	0.61	-2.9
5,324,785	-		BT4081	0.63	+0.8
5,324,850	+		BT4081	0.65	+0.1
5,324,851	-		BT4081	0.65	+3.5
5,324,851	-		BT4081	0.65	-0.2
5,324,881	-		BT4081	0.66	-1.1
5,325,020	-		BT4081	0.71	+0.4
5,325,024	-		BT4081	0.71	-0.2
5,325,024	-		BT4081	0.71	-2.9
5,325,052	-		BT4081	0.72	+1.3
5,325,061	-		BT4081	0.72	+1.7
5,325,098	-		BT4081	0.73	-4.1
5,325,107	+		BT4081	0.73	+1.7
5,325,195	+		BT4081	0.76	+1.1
5,325,195	+		BT4081	0.76	-0.7
5,325,196	-		BT4081	0.76	-1.2
5,325,230	+		BT4081	0.77	-0.9
5,325,231	-		BT4081	0.77	-3.0
5,325,325	-		BT4081	0.80	-0.9
5,325,338	+		BT4081	0.81	-0.4
5,325,399	-		BT4081	0.83	+0.7
5,325,413	-		BT4081	0.83	+0.1
5,325,481	-		BT4081	0.85	+1.0
5,325,481	-		BT4081	0.85	+3.5
5,325,492	-		BT4081	0.86	-3.2
5,325,561	-		BT4081	0.88	-0.3
5,325,608	-		BT4081	0.89	+1.7
5,325,749	-				-2.9
5,325,771	-				-3.0
5,325,905	-				-1.4
5,325,932	-				+2.1
5,325,941	+				+1.5
5,326,029	-				+0.6
5,326,038	+				+1.1
5,326,039	-				+2.5
5,326,046	+				+3.5
5,326,046	+				-0.1
5,326,219	+		BT4082	0.12	-1.7
5,326,220	-		BT4082	0.12	-2.7
5,326,228	+		BT4082	0.13	+1.5
5,326,229	-		BT4082	0.13	-1.0
5,326,229	-		BT4082	0.13	+3.6
5,326,230	+		BT4082	0.13	-2.6
5,326,231	-		BT4082	0.13	+2.5
5,326,307	-		BT4082	0.17	+3.5
5,326,324	+		BT4082	0.17	+2.7
5,326,349	-		BT4082	0.19	+0.1
5,326,361	-		BT4082	0.19	+1.7
5,326,426	-		BT4082	0.22	-1.4
5,326,433	+		BT4082	0.23	+1.1
5,326,434	-		BT4082	0.23	-2.2
5,326,440	-		BT4082	0.23	-0.7
5,326,480	-		BT4082	0.25	-0.6
5,326,496	-		BT4082	0.26	+3.1
5,326,499	-		BT4082	0.26	+0.7
5,326,520	+		BT4082	0.27	-1.9
5,326,521	-		BT4082	0.27	+2.0
5,326,537	+		BT4082	0.28	-0.6
5,326,537	+		BT4082	0.28	+2.5
5,326,538	-		BT4082	0.28	+0.1
5,326,542	-		BT4082	0.28	-0.7
5,326,627	+		BT4082	0.32	-2.7
5,326,692	+		BT4082	0.35	-0.9
5,326,692	+		BT4082	0.35	-3.2
5,326,694	+		BT4082	0.36	+2.2
5,326,760	+		BT4082	0.39	+1.6
5,326,760	+		BT4082	0.39	-3.2
5,326,846	-		BT4082	0.43	-4.2
5,326,846	-		BT4082	0.43	+3.6
5,326,850	-		BT4082	0.43	-1.2
5,326,850	-		BT4082	0.43	+0.6
5,326,865	-		BT4082	0.44	-0.2
5,326,895	+		BT4082	0.45	+2.8
5,326,900	+		BT4082	0.46	+2.5
5,326,904	+		BT4082	0.46	+1.3
5,326,904	+		BT4082	0.46	-3.7
5,326,904	+		BT4082	0.46	+0.8
5,326,904	+		BT4082	0.46	-1.2
5,326,905	-		BT4082	0.46	+1.1
5,326,905	-		BT4082	0.46	-1.1