Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3612

Experiment: BHIS with 5-Fluorouracil 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3611 and BT3612 are separated by 11 nucleotidesBT3612 and BT3613 are separated by 41 nucleotides BT3611: BT3611 - glycyl-tRNA synthetase (NCBI ptt file), at 4,679,697 to 4,681,238 BT3611 BT3612: BT3612 - putative FKBP-type peptidyl-prolyl cis-trans isomerase fkpA (NCBI ptt file), at 4,681,250 to 4,681,840 BT3612 BT3613: BT3613 - LacI family transcriptional regulator (NCBI ptt file), at 4,681,882 to 4,682,925 BT3613 Position (kb) 4681 4682Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 4681.231 kb on + strandat 4681.269 kb on + strandat 4681.278 kb on + strandat 4681.281 kb on + strandat 4681.282 kb on - strandat 4681.334 kb on + strand, within BT3612at 4681.367 kb on - strand, within BT3612at 4681.405 kb on + strand, within BT3612at 4681.425 kb on + strand, within BT3612at 4681.452 kb on - strand, within BT3612at 4681.453 kb on + strand, within BT3612at 4681.457 kb on + strand, within BT3612at 4681.458 kb on - strand, within BT3612at 4681.458 kb on - strand, within BT3612at 4681.460 kb on + strand, within BT3612at 4681.461 kb on - strand, within BT3612at 4681.461 kb on - strand, within BT3612at 4681.462 kb on + strand, within BT3612at 4681.491 kb on + strand, within BT3612at 4681.491 kb on + strand, within BT3612at 4681.492 kb on - strand, within BT3612at 4681.511 kb on - strand, within BT3612at 4681.515 kb on - strand, within BT3612at 4681.516 kb on + strand, within BT3612at 4681.533 kb on + strand, within BT3612at 4681.534 kb on - strand, within BT3612at 4681.535 kb on + strand, within BT3612at 4681.536 kb on - strand, within BT3612at 4681.538 kb on + strand, within BT3612at 4681.602 kb on + strand, within BT3612at 4681.721 kb on + strand, within BT3612at 4681.721 kb on + strand, within BT3612at 4681.721 kb on + strand, within BT3612at 4681.722 kb on - strand, within BT3612at 4681.736 kb on + strand, within BT3612at 4681.742 kb on + strand, within BT3612at 4681.751 kb on - strand, within BT3612at 4681.756 kb on + strand, within BT3612at 4681.757 kb on - strand, within BT3612at 4681.757 kb on - strand, within BT3612at 4681.819 kb on + strandat 4681.846 kb on + strandat 4681.857 kb on - strandat 4681.858 kb on + strandat 4681.862 kb on + strandat 4681.862 kb on + strandat 4681.878 kb on + strandat 4681.878 kb on + strandat 4681.879 kb on - strandat 4681.879 kb on - strandat 4681.880 kb on + strandat 4681.881 kb on - strandat 4681.883 kb on + strandat 4681.883 kb on + strandat 4681.887 kb on + strandat 4681.887 kb on + strandat 4681.888 kb on - strandat 4681.924 kb on - strandat 4681.983 kb on + strandat 4681.983 kb on + strandat 4682.028 kb on - strand, within BT3613at 4682.028 kb on - strand, within BT3613at 4682.050 kb on + strand, within BT3613at 4682.051 kb on - strand, within BT3613at 4682.066 kb on + strand, within BT3613at 4682.066 kb on + strand, within BT3613at 4682.140 kb on + strand, within BT3613at 4682.140 kb on + strand, within BT3613at 4682.140 kb on + strand, within BT3613at 4682.209 kb on + strand, within BT3613at 4682.210 kb on - strand, within BT3613at 4682.210 kb on - strand, within BT3613at 4682.212 kb on + strand, within BT3613at 4682.212 kb on + strand, within BT3613at 4682.213 kb on - strand, within BT3613at 4682.213 kb on - strand, within BT3613at 4682.213 kb on - strand, within BT3613at 4682.266 kb on + strand, within BT3613at 4682.312 kb on + strand, within BT3613at 4682.376 kb on + strand, within BT3613at 4682.377 kb on - strand, within BT3613at 4682.434 kb on + strand, within BT3613at 4682.491 kb on - strand, within BT3613at 4682.590 kb on - strand, within BT3613

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM
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4,681,231 + -1.5
4,681,269 + -1.4
4,681,278 + -1.5
4,681,281 + -0.4
4,681,282 - +1.8
4,681,334 + BT3612 0.14 -0.1
4,681,367 - BT3612 0.20 -0.8
4,681,405 + BT3612 0.26 +2.2
4,681,425 + BT3612 0.30 +2.0
4,681,452 - BT3612 0.34 -1.5
4,681,453 + BT3612 0.34 +2.6
4,681,457 + BT3612 0.35 -0.7
4,681,458 - BT3612 0.35 -0.9
4,681,458 - BT3612 0.35 +0.5
4,681,460 + BT3612 0.36 -0.5
4,681,461 - BT3612 0.36 +2.2
4,681,461 - BT3612 0.36 -0.6
4,681,462 + BT3612 0.36 +1.8
4,681,491 + BT3612 0.41 -1.2
4,681,491 + BT3612 0.41 +2.6
4,681,492 - BT3612 0.41 -0.3
4,681,511 - BT3612 0.44 -1.8
4,681,515 - BT3612 0.45 -3.4
4,681,516 + BT3612 0.45 +1.4
4,681,533 + BT3612 0.48 +1.8
4,681,534 - BT3612 0.48 -2.3
4,681,535 + BT3612 0.48 +1.0
4,681,536 - BT3612 0.48 +1.1
4,681,538 + BT3612 0.49 +1.2
4,681,602 + BT3612 0.60 +0.2
4,681,721 + BT3612 0.80 -0.2
4,681,721 + BT3612 0.80 +0.8
4,681,721 + BT3612 0.80 +1.6
4,681,722 - BT3612 0.80 -2.6
4,681,736 + BT3612 0.82 -3.0
4,681,742 + BT3612 0.83 +0.2
4,681,751 - BT3612 0.85 -1.1
4,681,756 + BT3612 0.86 -3.3
4,681,757 - BT3612 0.86 -1.7
4,681,757 - BT3612 0.86 -1.9
4,681,819 + -0.4
4,681,846 + +1.8
4,681,857 - +0.0
4,681,858 + -2.5
4,681,862 + -0.4
4,681,862 + +3.0
4,681,878 + -2.1
4,681,878 + -2.6
4,681,879 - -2.0
4,681,879 - -2.1
4,681,880 + -1.4
4,681,881 - +1.0
4,681,883 + +2.7
4,681,883 + -0.8
4,681,887 + +2.2
4,681,887 + -2.2
4,681,888 - -1.9
4,681,924 - -4.7
4,681,983 + +1.2
4,681,983 + -4.8
4,682,028 - BT3613 0.14 -1.9
4,682,028 - BT3613 0.14 -1.9
4,682,050 + BT3613 0.16 -1.0
4,682,051 - BT3613 0.16 -0.3
4,682,066 + BT3613 0.18 -2.5
4,682,066 + BT3613 0.18 -1.0
4,682,140 + BT3613 0.25 -2.7
4,682,140 + BT3613 0.25 -1.4
4,682,140 + BT3613 0.25 -4.7
4,682,209 + BT3613 0.31 -2.4
4,682,210 - BT3613 0.31 -2.3
4,682,210 - BT3613 0.31 -0.2
4,682,212 + BT3613 0.32 +1.7
4,682,212 + BT3613 0.32 -0.4
4,682,213 - BT3613 0.32 +0.7
4,682,213 - BT3613 0.32 +3.2
4,682,213 - BT3613 0.32 -1.1
4,682,266 + BT3613 0.37 -2.1
4,682,312 + BT3613 0.41 +3.7
4,682,376 + BT3613 0.47 -0.2
4,682,377 - BT3613 0.47 -0.5
4,682,434 + BT3613 0.53 +2.2
4,682,491 - BT3613 0.58 -1.8
4,682,590 - BT3613 0.68 -1.7

Or see this region's nucleotide sequence