Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3611 and BT3612 are separated by 11 nucleotides BT3612 and BT3613 are separated by 41 nucleotides
BT3611: BT3611 - glycyl-tRNA synthetase (NCBI ptt file), at 4,679,697 to 4,681,238
BT3611
BT3612: BT3612 - putative FKBP-type peptidyl-prolyl cis-trans isomerase fkpA (NCBI ptt file), at 4,681,250 to 4,681,840
BT3612
BT3613: BT3613 - LacI family transcriptional regulator (NCBI ptt file), at 4,681,882 to 4,682,925
BT3613
Position (kb)
4681
4682 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4681.231 kb on + strand at 4681.269 kb on + strand at 4681.278 kb on + strand at 4681.281 kb on + strand at 4681.282 kb on - strand at 4681.334 kb on + strand, within BT3612 at 4681.367 kb on - strand, within BT3612 at 4681.405 kb on + strand, within BT3612 at 4681.425 kb on + strand, within BT3612 at 4681.452 kb on - strand, within BT3612 at 4681.453 kb on + strand, within BT3612 at 4681.457 kb on + strand, within BT3612 at 4681.458 kb on - strand, within BT3612 at 4681.458 kb on - strand, within BT3612 at 4681.460 kb on + strand, within BT3612 at 4681.461 kb on - strand, within BT3612 at 4681.461 kb on - strand, within BT3612 at 4681.462 kb on + strand, within BT3612 at 4681.491 kb on + strand, within BT3612 at 4681.491 kb on + strand, within BT3612 at 4681.492 kb on - strand, within BT3612 at 4681.511 kb on - strand, within BT3612 at 4681.515 kb on - strand, within BT3612 at 4681.516 kb on + strand, within BT3612 at 4681.533 kb on + strand, within BT3612 at 4681.534 kb on - strand, within BT3612 at 4681.535 kb on + strand, within BT3612 at 4681.536 kb on - strand, within BT3612 at 4681.538 kb on + strand, within BT3612 at 4681.602 kb on + strand, within BT3612 at 4681.721 kb on + strand, within BT3612 at 4681.721 kb on + strand, within BT3612 at 4681.721 kb on + strand, within BT3612 at 4681.722 kb on - strand, within BT3612 at 4681.736 kb on + strand, within BT3612 at 4681.742 kb on + strand, within BT3612 at 4681.751 kb on - strand, within BT3612 at 4681.756 kb on + strand, within BT3612 at 4681.757 kb on - strand, within BT3612 at 4681.757 kb on - strand, within BT3612 at 4681.819 kb on + strand at 4681.846 kb on + strand at 4681.857 kb on - strand at 4681.858 kb on + strand at 4681.862 kb on + strand at 4681.862 kb on + strand at 4681.878 kb on + strand at 4681.878 kb on + strand at 4681.879 kb on - strand at 4681.879 kb on - strand at 4681.880 kb on + strand at 4681.881 kb on - strand at 4681.883 kb on + strand at 4681.883 kb on + strand at 4681.887 kb on + strand at 4681.887 kb on + strand at 4681.888 kb on - strand at 4681.924 kb on - strand at 4681.983 kb on + strand at 4681.983 kb on + strand at 4682.028 kb on - strand, within BT3613 at 4682.028 kb on - strand, within BT3613 at 4682.050 kb on + strand, within BT3613 at 4682.051 kb on - strand, within BT3613 at 4682.066 kb on + strand, within BT3613 at 4682.066 kb on + strand, within BT3613 at 4682.140 kb on + strand, within BT3613 at 4682.140 kb on + strand, within BT3613 at 4682.140 kb on + strand, within BT3613 at 4682.209 kb on + strand, within BT3613 at 4682.210 kb on - strand, within BT3613 at 4682.210 kb on - strand, within BT3613 at 4682.212 kb on + strand, within BT3613 at 4682.212 kb on + strand, within BT3613 at 4682.213 kb on - strand, within BT3613 at 4682.213 kb on - strand, within BT3613 at 4682.213 kb on - strand, within BT3613 at 4682.266 kb on + strand, within BT3613 at 4682.312 kb on + strand, within BT3613 at 4682.376 kb on + strand, within BT3613 at 4682.377 kb on - strand, within BT3613 at 4682.434 kb on + strand, within BT3613 at 4682.491 kb on - strand, within BT3613 at 4682.590 kb on - strand, within BT3613
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 4,681,231 + -1.5 4,681,269 + -1.4 4,681,278 + -1.5 4,681,281 + -0.4 4,681,282 - +1.8 4,681,334 + BT3612 0.14 -0.1 4,681,367 - BT3612 0.20 -0.8 4,681,405 + BT3612 0.26 +2.2 4,681,425 + BT3612 0.30 +2.0 4,681,452 - BT3612 0.34 -1.5 4,681,453 + BT3612 0.34 +2.6 4,681,457 + BT3612 0.35 -0.7 4,681,458 - BT3612 0.35 -0.9 4,681,458 - BT3612 0.35 +0.5 4,681,460 + BT3612 0.36 -0.5 4,681,461 - BT3612 0.36 +2.2 4,681,461 - BT3612 0.36 -0.6 4,681,462 + BT3612 0.36 +1.8 4,681,491 + BT3612 0.41 -1.2 4,681,491 + BT3612 0.41 +2.6 4,681,492 - BT3612 0.41 -0.3 4,681,511 - BT3612 0.44 -1.8 4,681,515 - BT3612 0.45 -3.4 4,681,516 + BT3612 0.45 +1.4 4,681,533 + BT3612 0.48 +1.8 4,681,534 - BT3612 0.48 -2.3 4,681,535 + BT3612 0.48 +1.0 4,681,536 - BT3612 0.48 +1.1 4,681,538 + BT3612 0.49 +1.2 4,681,602 + BT3612 0.60 +0.2 4,681,721 + BT3612 0.80 -0.2 4,681,721 + BT3612 0.80 +0.8 4,681,721 + BT3612 0.80 +1.6 4,681,722 - BT3612 0.80 -2.6 4,681,736 + BT3612 0.82 -3.0 4,681,742 + BT3612 0.83 +0.2 4,681,751 - BT3612 0.85 -1.1 4,681,756 + BT3612 0.86 -3.3 4,681,757 - BT3612 0.86 -1.7 4,681,757 - BT3612 0.86 -1.9 4,681,819 + -0.4 4,681,846 + +1.8 4,681,857 - +0.0 4,681,858 + -2.5 4,681,862 + -0.4 4,681,862 + +3.0 4,681,878 + -2.1 4,681,878 + -2.6 4,681,879 - -2.0 4,681,879 - -2.1 4,681,880 + -1.4 4,681,881 - +1.0 4,681,883 + +2.7 4,681,883 + -0.8 4,681,887 + +2.2 4,681,887 + -2.2 4,681,888 - -1.9 4,681,924 - -4.7 4,681,983 + +1.2 4,681,983 + -4.8 4,682,028 - BT3613 0.14 -1.9 4,682,028 - BT3613 0.14 -1.9 4,682,050 + BT3613 0.16 -1.0 4,682,051 - BT3613 0.16 -0.3 4,682,066 + BT3613 0.18 -2.5 4,682,066 + BT3613 0.18 -1.0 4,682,140 + BT3613 0.25 -2.7 4,682,140 + BT3613 0.25 -1.4 4,682,140 + BT3613 0.25 -4.7 4,682,209 + BT3613 0.31 -2.4 4,682,210 - BT3613 0.31 -2.3 4,682,210 - BT3613 0.31 -0.2 4,682,212 + BT3613 0.32 +1.7 4,682,212 + BT3613 0.32 -0.4 4,682,213 - BT3613 0.32 +0.7 4,682,213 - BT3613 0.32 +3.2 4,682,213 - BT3613 0.32 -1.1 4,682,266 + BT3613 0.37 -2.1 4,682,312 + BT3613 0.41 +3.7 4,682,376 + BT3613 0.47 -0.2 4,682,377 - BT3613 0.47 -0.5 4,682,434 + BT3613 0.53 +2.2 4,682,491 - BT3613 0.58 -1.8 4,682,590 - BT3613 0.68 -1.7
Or see this region's nucleotide sequence