Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3500

Experiment: BHIS with 5-Fluorouracil 0.1 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	BHIS with 5-Fluorouracil 0.1 mM
4,512,769	-		BT3498	0.81	+1.0
4,512,788	-		BT3498	0.84	-0.2
4,512,819	+		BT3498	0.88	-2.4
4,512,820	-		BT3498	0.88	+2.6
4,512,856	-				-1.6
4,512,865	-				+1.2
4,512,931	-				-0.3
4,512,969	+		BT3499	0.11	+1.8
4,513,090	-		BT3499	0.27	-0.1
4,513,090	-		BT3499	0.27	+1.6
4,513,131	-		BT3499	0.33	+2.5
4,513,131	-		BT3499	0.33	-2.0
4,513,136	+		BT3499	0.33	+3.5
4,513,136	+		BT3499	0.33	+1.4
4,513,136	+		BT3499	0.33	-4.4
4,513,137	-		BT3499	0.34	-1.3
4,513,137	-		BT3499	0.34	-1.4
4,513,147	+		BT3499	0.35	+3.6
4,513,184	-		BT3499	0.40	-0.1
4,513,184	-		BT3499	0.40	-2.4
4,513,194	-		BT3499	0.41	-2.2
4,513,250	-		BT3499	0.49	+1.2
4,513,283	+		BT3499	0.53	-0.8
4,513,284	-		BT3499	0.54	-1.4
4,513,284	-		BT3499	0.54	+0.2
4,513,284	-		BT3499	0.54	-3.0
4,513,286	-		BT3499	0.54	+0.9
4,513,286	-		BT3499	0.54	+0.2
4,513,289	+		BT3499	0.54	+2.0
4,513,340	-		BT3499	0.61	-1.5
4,513,403	+		BT3499	0.70	-1.4
4,513,403	+		BT3499	0.70	-0.1
4,513,404	-		BT3499	0.70	-3.0
4,513,404	-		BT3499	0.70	+1.8
4,513,408	+		BT3499	0.70	+1.5
4,513,414	+		BT3499	0.71	-2.8
4,513,500	+		BT3499	0.83	-0.8
4,513,501	-		BT3499	0.83	-1.0
4,513,501	-		BT3499	0.83	-1.4
4,513,609	+				-1.1
4,513,610	-				+2.5
4,513,631	-				+2.2
4,513,726	-				-2.8
4,513,745	+				+1.3
4,513,791	+				+2.0
4,513,792	-				+1.5
4,513,821	+				+0.1
4,513,863	+				+3.6
4,513,864	-				+1.8
4,513,983	+		BT3500	0.13	+1.2
4,513,984	-		BT3500	0.13	+2.8
4,513,984	-		BT3500	0.13	+3.2
4,514,035	-		BT3500	0.16	+3.3
4,514,038	+		BT3500	0.16	+3.3
4,514,038	+		BT3500	0.16	-3.5
4,514,039	-		BT3500	0.16	-1.0
4,514,066	+		BT3500	0.18	-0.3
4,514,070	-		BT3500	0.18	-0.7
4,514,070	-		BT3500	0.18	-1.1
4,514,077	+		BT3500	0.18	-1.8
4,514,077	+		BT3500	0.18	-0.9
4,514,077	+		BT3500	0.18	+1.2
4,514,080	-		BT3500	0.18	-3.7
4,514,083	+		BT3500	0.19	-1.8
4,514,083	+		BT3500	0.19	-0.5
4,514,143	+		BT3500	0.22	+2.5
4,514,182	+		BT3500	0.24	+0.2
4,514,185	-		BT3500	0.24	+2.9
4,514,215	-		BT3500	0.26	+1.4
4,514,236	+		BT3500	0.27	+3.4
4,514,239	+		BT3500	0.28	+0.4
4,514,251	+		BT3500	0.28	-2.0
4,514,326	+		BT3500	0.33	+2.3
4,514,399	+		BT3500	0.37	+2.1
4,514,433	+		BT3500	0.39	-2.3
4,514,545	+		BT3500	0.45	-0.4
4,514,545	+		BT3500	0.45	+0.2
4,514,546	-		BT3500	0.45	-1.4
4,514,546	-		BT3500	0.45	+0.8
4,514,615	+		BT3500	0.49	-2.5
4,514,618	+		BT3500	0.49	-1.2
4,514,619	-		BT3500	0.49	-1.8
4,514,623	+		BT3500	0.50	+0.2
4,514,651	-		BT3500	0.51	-3.0
4,514,703	-		BT3500	0.54	+1.8
4,514,720	-		BT3500	0.55	-1.6
4,514,766	-		BT3500	0.58	-1.2
4,514,774	-		BT3500	0.58	+1.2
4,514,820	-		BT3500	0.61	-0.7
4,514,820	-		BT3500	0.61	-2.6
4,514,821	+		BT3500	0.61	+2.2
4,514,954	+		BT3500	0.68	+3.0
4,515,001	+		BT3500	0.71	-2.8
4,515,001	+		BT3500	0.71	-1.3
4,515,004	+		BT3500	0.71	-1.0
4,515,021	-		BT3500	0.72	-1.4
4,515,056	+		BT3500	0.74	-1.1
4,515,056	+		BT3500	0.74	+2.4
4,515,057	-		BT3500	0.74	+0.9
4,515,057	-		BT3500	0.74	+3.5
4,515,064	-		BT3500	0.75	+1.4
4,515,115	+		BT3500	0.78	-2.6
4,515,236	-		BT3500	0.85	+2.2
4,515,298	+		BT3500	0.88	-3.2
4,515,334	-				+2.2
4,515,338	+				-1.2
4,515,338	+				-0.8
4,515,342	-				-2.6
4,515,406	+				-1.4
4,515,410	+				-2.4
4,515,416	+				-2.2
4,515,444	-				+2.5
4,515,459	-				-3.7
4,515,503	+				-1.4
4,515,512	-				+1.6
4,515,513	+				-1.4
4,515,514	-				-0.6
4,515,541	+				-1.8
4,515,567	-				-1.3
4,515,579	+				-0.8
4,515,580	-				-2.1
4,515,707	+		BT3501	0.13	+1.4
4,515,708	-		BT3501	0.13	-1.2
4,515,753	-		BT3501	0.16	-0.9
4,515,763	+		BT3501	0.17	+1.8
4,515,783	+		BT3501	0.18	-0.6
4,515,805	-		BT3501	0.19	+3.3
4,515,843	-		BT3501	0.22	+3.5
4,515,843	-		BT3501	0.22	+3.0
4,515,856	+		BT3501	0.23	-1.2
4,515,887	+		BT3501	0.25	+2.0
4,515,892	-		BT3501	0.25	+1.7
4,515,892	-		BT3501	0.25	+0.2
4,515,892	-		BT3501	0.25	+0.0
4,515,895	+		BT3501	0.26	+1.3
4,515,896	-		BT3501	0.26	+2.3
4,515,923	+		BT3501	0.27	-0.8
4,515,984	+		BT3501	0.32	+2.9
4,515,984	+		BT3501	0.32	+1.2
4,515,984	+		BT3501	0.32	-1.1
4,515,984	+		BT3501	0.32	+0.8
4,515,985	-		BT3501	0.32	-2.6
4,515,985	-		BT3501	0.32	-0.8
4,515,985	-		BT3501	0.32	-2.0
4,515,988	+		BT3501	0.32	-1.4
4,515,988	+		BT3501	0.32	-2.2
4,515,989	-		BT3501	0.32	-1.1
4,515,999	-		BT3501	0.33	-1.5
4,516,050	+		BT3501	0.36	+2.4
4,516,050	+		BT3501	0.36	-1.0
4,516,051	-		BT3501	0.36	+1.8
4,516,069	+		BT3501	0.37	-1.4
4,516,076	-		BT3501	0.38	-0.4
4,516,128	+		BT3501	0.41	+1.3
4,516,128	+		BT3501	0.41	-2.2
4,516,155	+		BT3501	0.43	+2.2
4,516,184	-		BT3501	0.45	-1.0
4,516,204	+		BT3501	0.46	+0.6
4,516,213	-		BT3501	0.47	-1.7
4,516,238	+		BT3501	0.49	-0.8
4,516,239	-		BT3501	0.49	-2.6
4,516,283	+		BT3501	0.52	+0.2
4,516,283	+		BT3501	0.52	-0.5
4,516,340	-		BT3501	0.56	-1.4
4,516,353	+		BT3501	0.57	+1.8
4,516,356	+		BT3501	0.57	-1.4
4,516,432	-		BT3501	0.62	-1.4
4,516,432	-		BT3501	0.62	-2.0
4,516,459	-		BT3501	0.64	-2.2
4,516,465	+		BT3501	0.64	+2.7
4,516,474	+		BT3501	0.65	-1.4
4,516,493	+		BT3501	0.66	-2.8
4,516,494	-		BT3501	0.66	+2.2