Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3462 and BT3463 overlap by 4 nucleotides BT3463 and BT3464 overlap by 4 nucleotides BT3464 and BT3465 are separated by 23 nucleotides
BT3462: BT3462 - TPR domain protein (NCBI ptt file), at 4,457,500 to 4,459,254
BT3462
BT3463: BT3463 - hypothetical protein (NCBI ptt file), at 4,459,251 to 4,459,832
BT3463
BT3464: BT3464 - putative peptidase (NCBI ptt file), at 4,459,829 to 4,461,136
BT3464
BT3465: BT3465 - two-component system sensor histidine kinase/response regulator, hybrid ('one-component system') (NCBI ptt file), at 4,461,160 to 4,465,197
BT3465
Position (kb)
4459
4460
4461
4462 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4459.015 kb on + strand, within BT3462 at 4459.045 kb on + strand, within BT3462 at 4459.131 kb on + strand at 4459.819 kb on + strand at 4459.828 kb on + strand at 4459.829 kb on - strand at 4459.993 kb on - strand, within BT3464 at 4459.993 kb on - strand, within BT3464 at 4459.993 kb on - strand, within BT3464 at 4460.097 kb on + strand, within BT3464 at 4460.183 kb on + strand, within BT3464 at 4460.184 kb on - strand, within BT3464 at 4460.186 kb on - strand, within BT3464 at 4460.186 kb on - strand, within BT3464 at 4460.249 kb on + strand, within BT3464 at 4460.441 kb on - strand, within BT3464 at 4460.441 kb on - strand, within BT3464 at 4460.556 kb on + strand, within BT3464 at 4460.762 kb on - strand, within BT3464 at 4460.775 kb on - strand, within BT3464 at 4460.775 kb on - strand, within BT3464 at 4460.777 kb on - strand, within BT3464 at 4460.802 kb on - strand, within BT3464 at 4460.854 kb on - strand, within BT3464 at 4460.975 kb on - strand, within BT3464 at 4461.040 kb on - strand at 4461.073 kb on - strand at 4461.140 kb on - strand at 4461.144 kb on - strand at 4461.167 kb on + strand at 4461.273 kb on + strand at 4461.274 kb on - strand at 4461.274 kb on - strand at 4461.279 kb on - strand at 4461.279 kb on - strand at 4461.288 kb on + strand at 4461.289 kb on - strand at 4461.289 kb on - strand at 4461.290 kb on + strand at 4461.291 kb on - strand at 4461.302 kb on - strand at 4461.360 kb on + strand at 4461.441 kb on + strand at 4461.442 kb on - strand at 4461.443 kb on + strand at 4461.469 kb on + strand at 4461.469 kb on + strand at 4461.469 kb on + strand at 4461.469 kb on + strand at 4461.471 kb on + strand at 4461.471 kb on + strand at 4461.476 kb on - strand at 4461.516 kb on + strand at 4461.533 kb on - strand at 4461.560 kb on + strand at 4461.560 kb on + strand at 4461.561 kb on - strand at 4461.648 kb on + strand, within BT3465 at 4461.688 kb on + strand, within BT3465 at 4461.706 kb on + strand, within BT3465 at 4461.719 kb on + strand, within BT3465 at 4461.801 kb on + strand, within BT3465 at 4461.813 kb on - strand, within BT3465 at 4461.822 kb on - strand, within BT3465 at 4461.907 kb on + strand, within BT3465 at 4461.908 kb on - strand, within BT3465 at 4461.908 kb on - strand, within BT3465 at 4461.998 kb on - strand, within BT3465 at 4462.002 kb on - strand, within BT3465 at 4462.038 kb on + strand, within BT3465
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 4,459,015 + BT3462 0.86 +0.8 4,459,045 + BT3462 0.88 -0.8 4,459,131 + -3.0 4,459,819 + -2.6 4,459,828 + +2.8 4,459,829 - +2.2 4,459,993 - BT3464 0.13 +3.4 4,459,993 - BT3464 0.13 -2.1 4,459,993 - BT3464 0.13 -2.2 4,460,097 + BT3464 0.20 +3.5 4,460,183 + BT3464 0.27 +0.6 4,460,184 - BT3464 0.27 +1.8 4,460,186 - BT3464 0.27 +0.2 4,460,186 - BT3464 0.27 +2.9 4,460,249 + BT3464 0.32 +1.2 4,460,441 - BT3464 0.47 -2.5 4,460,441 - BT3464 0.47 +1.5 4,460,556 + BT3464 0.56 -3.4 4,460,762 - BT3464 0.71 +1.2 4,460,775 - BT3464 0.72 +0.2 4,460,775 - BT3464 0.72 +1.8 4,460,777 - BT3464 0.72 +2.5 4,460,802 - BT3464 0.74 +0.5 4,460,854 - BT3464 0.78 +0.9 4,460,975 - BT3464 0.88 +0.4 4,461,040 - +1.5 4,461,073 - +2.2 4,461,140 - -4.1 4,461,144 - +0.5 4,461,167 + +1.2 4,461,273 + -2.0 4,461,274 - +2.4 4,461,274 - -0.9 4,461,279 - +0.2 4,461,279 - -1.8 4,461,288 + +2.2 4,461,289 - -1.1 4,461,289 - +0.8 4,461,290 + -1.4 4,461,291 - -3.5 4,461,302 - -2.4 4,461,360 + -3.3 4,461,441 + -2.4 4,461,442 - +1.2 4,461,443 + +0.2 4,461,469 + +1.5 4,461,469 + +2.1 4,461,469 + +0.2 4,461,469 + +0.2 4,461,471 + -0.8 4,461,471 + +0.2 4,461,476 - -1.8 4,461,516 + -1.5 4,461,533 - -0.7 4,461,560 + -2.6 4,461,560 + -0.5 4,461,561 - +1.8 4,461,648 + BT3465 0.12 -2.3 4,461,688 + BT3465 0.13 -3.7 4,461,706 + BT3465 0.14 +2.8 4,461,719 + BT3465 0.14 +1.8 4,461,801 + BT3465 0.16 +1.6 4,461,813 - BT3465 0.16 -2.3 4,461,822 - BT3465 0.16 -1.3 4,461,907 + BT3465 0.18 -2.1 4,461,908 - BT3465 0.19 +1.8 4,461,908 - BT3465 0.19 +0.2 4,461,998 - BT3465 0.21 -1.6 4,462,002 - BT3465 0.21 +0.2 4,462,038 + BT3465 0.22 +0.2
Or see this region's nucleotide sequence