Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3464

Experiment: BHIS with 5-Fluorouracil 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3462 and BT3463 overlap by 4 nucleotidesBT3463 and BT3464 overlap by 4 nucleotidesBT3464 and BT3465 are separated by 23 nucleotides BT3462: BT3462 - TPR domain protein (NCBI ptt file), at 4,457,500 to 4,459,254 BT3462 BT3463: BT3463 - hypothetical protein (NCBI ptt file), at 4,459,251 to 4,459,832 BT3463 BT3464: BT3464 - putative peptidase (NCBI ptt file), at 4,459,829 to 4,461,136 BT3464 BT3465: BT3465 - two-component system sensor histidine kinase/response regulator, hybrid ('one-component system') (NCBI ptt file), at 4,461,160 to 4,465,197 BT3465 Position (kb) 4459 4460 4461 4462Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4459.015 kb on + strand, within BT3462at 4459.045 kb on + strand, within BT3462at 4459.131 kb on + strandat 4459.819 kb on + strandat 4459.828 kb on + strandat 4459.829 kb on - strandat 4459.993 kb on - strand, within BT3464at 4459.993 kb on - strand, within BT3464at 4459.993 kb on - strand, within BT3464at 4460.097 kb on + strand, within BT3464at 4460.183 kb on + strand, within BT3464at 4460.184 kb on - strand, within BT3464at 4460.186 kb on - strand, within BT3464at 4460.186 kb on - strand, within BT3464at 4460.249 kb on + strand, within BT3464at 4460.441 kb on - strand, within BT3464at 4460.441 kb on - strand, within BT3464at 4460.556 kb on + strand, within BT3464at 4460.762 kb on - strand, within BT3464at 4460.775 kb on - strand, within BT3464at 4460.775 kb on - strand, within BT3464at 4460.777 kb on - strand, within BT3464at 4460.802 kb on - strand, within BT3464at 4460.854 kb on - strand, within BT3464at 4460.975 kb on - strand, within BT3464at 4461.040 kb on - strandat 4461.073 kb on - strandat 4461.140 kb on - strandat 4461.144 kb on - strandat 4461.167 kb on + strandat 4461.273 kb on + strandat 4461.274 kb on - strandat 4461.274 kb on - strandat 4461.279 kb on - strandat 4461.279 kb on - strandat 4461.288 kb on + strandat 4461.289 kb on - strandat 4461.289 kb on - strandat 4461.290 kb on + strandat 4461.291 kb on - strandat 4461.302 kb on - strandat 4461.360 kb on + strandat 4461.441 kb on + strandat 4461.442 kb on - strandat 4461.443 kb on + strandat 4461.469 kb on + strandat 4461.469 kb on + strandat 4461.469 kb on + strandat 4461.469 kb on + strandat 4461.471 kb on + strandat 4461.471 kb on + strandat 4461.476 kb on - strandat 4461.516 kb on + strandat 4461.533 kb on - strandat 4461.560 kb on + strandat 4461.560 kb on + strandat 4461.561 kb on - strandat 4461.648 kb on + strand, within BT3465at 4461.688 kb on + strand, within BT3465at 4461.706 kb on + strand, within BT3465at 4461.719 kb on + strand, within BT3465at 4461.801 kb on + strand, within BT3465at 4461.813 kb on - strand, within BT3465at 4461.822 kb on - strand, within BT3465at 4461.907 kb on + strand, within BT3465at 4461.908 kb on - strand, within BT3465at 4461.908 kb on - strand, within BT3465at 4461.998 kb on - strand, within BT3465at 4462.002 kb on - strand, within BT3465at 4462.038 kb on + strand, within BT3465

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM
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4,459,015 + BT3462 0.86 +0.8
4,459,045 + BT3462 0.88 -0.8
4,459,131 + -3.0
4,459,819 + -2.6
4,459,828 + +2.8
4,459,829 - +2.2
4,459,993 - BT3464 0.13 +3.4
4,459,993 - BT3464 0.13 -2.1
4,459,993 - BT3464 0.13 -2.2
4,460,097 + BT3464 0.20 +3.5
4,460,183 + BT3464 0.27 +0.6
4,460,184 - BT3464 0.27 +1.8
4,460,186 - BT3464 0.27 +0.2
4,460,186 - BT3464 0.27 +2.9
4,460,249 + BT3464 0.32 +1.2
4,460,441 - BT3464 0.47 -2.5
4,460,441 - BT3464 0.47 +1.5
4,460,556 + BT3464 0.56 -3.4
4,460,762 - BT3464 0.71 +1.2
4,460,775 - BT3464 0.72 +0.2
4,460,775 - BT3464 0.72 +1.8
4,460,777 - BT3464 0.72 +2.5
4,460,802 - BT3464 0.74 +0.5
4,460,854 - BT3464 0.78 +0.9
4,460,975 - BT3464 0.88 +0.4
4,461,040 - +1.5
4,461,073 - +2.2
4,461,140 - -4.1
4,461,144 - +0.5
4,461,167 + +1.2
4,461,273 + -2.0
4,461,274 - +2.4
4,461,274 - -0.9
4,461,279 - +0.2
4,461,279 - -1.8
4,461,288 + +2.2
4,461,289 - -1.1
4,461,289 - +0.8
4,461,290 + -1.4
4,461,291 - -3.5
4,461,302 - -2.4
4,461,360 + -3.3
4,461,441 + -2.4
4,461,442 - +1.2
4,461,443 + +0.2
4,461,469 + +1.5
4,461,469 + +2.1
4,461,469 + +0.2
4,461,469 + +0.2
4,461,471 + -0.8
4,461,471 + +0.2
4,461,476 - -1.8
4,461,516 + -1.5
4,461,533 - -0.7
4,461,560 + -2.6
4,461,560 + -0.5
4,461,561 - +1.8
4,461,648 + BT3465 0.12 -2.3
4,461,688 + BT3465 0.13 -3.7
4,461,706 + BT3465 0.14 +2.8
4,461,719 + BT3465 0.14 +1.8
4,461,801 + BT3465 0.16 +1.6
4,461,813 - BT3465 0.16 -2.3
4,461,822 - BT3465 0.16 -1.3
4,461,907 + BT3465 0.18 -2.1
4,461,908 - BT3465 0.19 +1.8
4,461,908 - BT3465 0.19 +0.2
4,461,998 - BT3465 0.21 -1.6
4,462,002 - BT3465 0.21 +0.2
4,462,038 + BT3465 0.22 +0.2

Or see this region's nucleotide sequence