Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2787 and BT2788 are separated by 129 nucleotides BT2788 and BT2789 overlap by 4 nucleotides BT2789 and BT2790 are separated by 131 nucleotides
BT2787: BT2787 - GTP-binding elongation factor family protein TypA/BipA (NCBI ptt file), at 3,456,602 to 3,458,401
BT2787
BT2788: BT2788 - chromate transport protein (NCBI ptt file), at 3,458,531 to 3,459,055
BT2788
BT2789: BT2789 - chromate transport protein (NCBI ptt file), at 3,459,052 to 3,459,609
BT2789
BT2790: BT2790 - phosphoenolpyruvate carboxykinase [ATP] (NCBI ptt file), at 3,459,741 to 3,461,348
BT2790
Position (kb)
3458
3459
3460 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3457.636 kb on - strand, within BT2787 at 3457.684 kb on - strand, within BT2787 at 3457.684 kb on - strand, within BT2787 at 3457.698 kb on + strand, within BT2787 at 3457.757 kb on - strand, within BT2787 at 3457.826 kb on + strand, within BT2787 at 3457.827 kb on - strand, within BT2787 at 3457.840 kb on + strand, within BT2787 at 3457.841 kb on - strand, within BT2787 at 3457.900 kb on + strand, within BT2787 at 3457.901 kb on - strand, within BT2787 at 3457.933 kb on + strand, within BT2787 at 3457.933 kb on + strand, within BT2787 at 3457.956 kb on + strand, within BT2787 at 3457.981 kb on + strand, within BT2787 at 3457.981 kb on + strand, within BT2787 at 3457.982 kb on - strand, within BT2787 at 3457.982 kb on - strand, within BT2787 at 3457.982 kb on - strand, within BT2787 at 3458.038 kb on + strand, within BT2787 at 3458.084 kb on + strand, within BT2787 at 3458.085 kb on - strand, within BT2787 at 3458.142 kb on + strand, within BT2787 at 3458.145 kb on - strand, within BT2787 at 3458.160 kb on + strand, within BT2787 at 3458.200 kb on + strand, within BT2787 at 3458.318 kb on + strand at 3458.318 kb on + strand at 3458.319 kb on - strand at 3458.319 kb on - strand at 3458.319 kb on - strand at 3458.319 kb on - strand at 3458.319 kb on - strand at 3458.347 kb on + strand at 3458.348 kb on - strand at 3458.400 kb on - strand at 3458.407 kb on - strand at 3458.417 kb on - strand at 3458.476 kb on - strand at 3458.476 kb on - strand at 3458.476 kb on - strand at 3458.478 kb on + strand at 3458.491 kb on + strand at 3458.532 kb on + strand at 3458.578 kb on + strand at 3458.605 kb on + strand, within BT2788 at 3458.631 kb on - strand, within BT2788 at 3458.633 kb on - strand at 3458.639 kb on - strand, within BT2788 at 3458.640 kb on + strand, within BT2788 at 3458.641 kb on - strand, within BT2788 at 3458.733 kb on - strand at 3458.733 kb on - strand, within BT2788 at 3458.736 kb on + strand, within BT2788 at 3458.736 kb on + strand, within BT2788 at 3458.822 kb on + strand, within BT2788 at 3458.822 kb on + strand, within BT2788 at 3458.822 kb on + strand, within BT2788 at 3458.854 kb on - strand, within BT2788 at 3458.862 kb on + strand, within BT2788 at 3458.863 kb on - strand, within BT2788 at 3458.863 kb on - strand, within BT2788 at 3458.866 kb on + strand, within BT2788 at 3458.866 kb on + strand, within BT2788 at 3458.872 kb on + strand, within BT2788 at 3458.873 kb on - strand, within BT2788 at 3458.876 kb on - strand, within BT2788 at 3458.876 kb on - strand, within BT2788 at 3458.991 kb on + strand, within BT2788 at 3459.013 kb on + strand at 3459.014 kb on - strand at 3459.014 kb on - strand at 3459.027 kb on + strand at 3459.048 kb on + strand at 3459.048 kb on + strand at 3459.049 kb on - strand at 3459.050 kb on + strand at 3459.051 kb on - strand at 3459.062 kb on - strand at 3459.070 kb on - strand at 3459.093 kb on + strand at 3459.100 kb on + strand at 3459.135 kb on + strand, within BT2789 at 3459.136 kb on - strand, within BT2789 at 3459.197 kb on + strand, within BT2789 at 3459.197 kb on + strand, within BT2789 at 3459.226 kb on + strand, within BT2789 at 3459.227 kb on - strand, within BT2789 at 3459.227 kb on - strand, within BT2789 at 3459.228 kb on + strand, within BT2789 at 3459.265 kb on + strand, within BT2789 at 3459.337 kb on - strand, within BT2789 at 3459.357 kb on + strand, within BT2789 at 3459.357 kb on + strand, within BT2789 at 3459.358 kb on - strand, within BT2789 at 3459.394 kb on - strand, within BT2789 at 3459.394 kb on - strand, within BT2789 at 3459.394 kb on - strand, within BT2789 at 3459.403 kb on - strand, within BT2789 at 3459.421 kb on + strand, within BT2789 at 3459.423 kb on + strand, within BT2789 at 3459.423 kb on + strand, within BT2789 at 3459.423 kb on + strand, within BT2789 at 3459.424 kb on - strand, within BT2789 at 3459.424 kb on - strand, within BT2789 at 3459.424 kb on - strand, within BT2789 at 3459.520 kb on + strand, within BT2789 at 3459.556 kb on - strand at 3459.603 kb on + strand at 3459.604 kb on - strand at 3459.604 kb on - strand at 3459.604 kb on - strand at 3459.635 kb on + strand at 3459.670 kb on + strand at 3459.670 kb on + strand at 3459.670 kb on + strand at 3459.671 kb on - strand at 3459.671 kb on - strand at 3459.672 kb on + strand at 3459.673 kb on - strand at 3459.719 kb on - strand at 3459.723 kb on - strand at 3459.723 kb on - strand at 3459.723 kb on - strand at 3459.727 kb on - strand at 3459.727 kb on - strand at 3459.742 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 3,457,636 - BT2787 0.57 -2.8 3,457,684 - BT2787 0.60 -2.7 3,457,684 - BT2787 0.60 -1.5 3,457,698 + BT2787 0.61 -2.9 3,457,757 - BT2787 0.64 -4.1 3,457,826 + BT2787 0.68 -3.6 3,457,827 - BT2787 0.68 -1.3 3,457,840 + BT2787 0.69 +0.4 3,457,841 - BT2787 0.69 -3.7 3,457,900 + BT2787 0.72 -3.0 3,457,901 - BT2787 0.72 -3.1 3,457,933 + BT2787 0.74 -2.7 3,457,933 + BT2787 0.74 -0.7 3,457,956 + BT2787 0.75 -2.4 3,457,981 + BT2787 0.77 -2.0 3,457,981 + BT2787 0.77 -3.0 3,457,982 - BT2787 0.77 +0.1 3,457,982 - BT2787 0.77 -2.2 3,457,982 - BT2787 0.77 +1.0 3,458,038 + BT2787 0.80 -4.0 3,458,084 + BT2787 0.82 -1.5 3,458,085 - BT2787 0.82 -3.6 3,458,142 + BT2787 0.86 +1.1 3,458,145 - BT2787 0.86 -5.0 3,458,160 + BT2787 0.87 -2.2 3,458,200 + BT2787 0.89 -0.2 3,458,318 + -1.1 3,458,318 + -3.1 3,458,319 - -2.4 3,458,319 - -2.7 3,458,319 - -3.6 3,458,319 - +0.1 3,458,319 - +1.1 3,458,347 + -0.9 3,458,348 - -2.8 3,458,400 - +3.3 3,458,407 - -1.1 3,458,417 - -1.5 3,458,476 - -2.3 3,458,476 - +0.1 3,458,476 - +1.1 3,458,478 + +1.4 3,458,491 + +1.5 3,458,532 + +0.9 3,458,578 + -1.5 3,458,605 + BT2788 0.14 -1.8 3,458,631 - BT2788 0.19 +1.1 3,458,633 - -2.9 3,458,639 - BT2788 0.21 -2.7 3,458,640 + BT2788 0.21 -2.4 3,458,641 - BT2788 0.21 -3.3 3,458,733 - +1.1 3,458,733 - BT2788 0.38 -0.2 3,458,736 + BT2788 0.39 +1.7 3,458,736 + BT2788 0.39 +0.1 3,458,822 + BT2788 0.55 -1.5 3,458,822 + BT2788 0.55 -1.8 3,458,822 + BT2788 0.55 -2.5 3,458,854 - BT2788 0.62 +0.3 3,458,862 + BT2788 0.63 -2.9 3,458,863 - BT2788 0.63 +0.1 3,458,863 - BT2788 0.63 +2.4 3,458,866 + BT2788 0.64 +1.1 3,458,866 + BT2788 0.64 -3.8 3,458,872 + BT2788 0.65 -1.9 3,458,873 - BT2788 0.65 +0.1 3,458,876 - BT2788 0.66 +0.1 3,458,876 - BT2788 0.66 -0.9 3,458,991 + BT2788 0.88 -3.5 3,459,013 + +2.3 3,459,014 - -2.5 3,459,014 - +1.1 3,459,027 + -0.6 3,459,048 + +0.4 3,459,048 + -2.7 3,459,049 - +2.2 3,459,050 + +2.8 3,459,051 - -4.1 3,459,062 - -2.1 3,459,070 - -3.0 3,459,093 + +2.3 3,459,100 + -1.5 3,459,135 + BT2789 0.15 +1.1 3,459,136 - BT2789 0.15 +2.7 3,459,197 + BT2789 0.26 -1.4 3,459,197 + BT2789 0.26 +2.1 3,459,226 + BT2789 0.31 +1.1 3,459,227 - BT2789 0.31 +0.8 3,459,227 - BT2789 0.31 -1.6 3,459,228 + BT2789 0.32 +0.6 3,459,265 + BT2789 0.38 -0.7 3,459,337 - BT2789 0.51 -3.2 3,459,357 + BT2789 0.55 -0.4 3,459,357 + BT2789 0.55 +0.1 3,459,358 - BT2789 0.55 -1.1 3,459,394 - BT2789 0.61 +0.1 3,459,394 - BT2789 0.61 -1.2 3,459,394 - BT2789 0.61 -1.1 3,459,403 - BT2789 0.63 -1.5 3,459,421 + BT2789 0.66 -1.5 3,459,423 + BT2789 0.66 -1.6 3,459,423 + BT2789 0.66 -1.6 3,459,423 + BT2789 0.66 +0.1 3,459,424 - BT2789 0.67 +3.1 3,459,424 - BT2789 0.67 +0.1 3,459,424 - BT2789 0.67 -2.3 3,459,520 + BT2789 0.84 +1.1 3,459,556 - +3.1 3,459,603 + +0.1 3,459,604 - +3.2 3,459,604 - -1.4 3,459,604 - -1.8 3,459,635 + +2.1 3,459,670 + -1.3 3,459,670 + +0.5 3,459,670 + -0.2 3,459,671 - +0.1 3,459,671 - +0.1 3,459,672 + -2.0 3,459,673 - +0.9 3,459,719 - -3.6 3,459,723 - +1.1 3,459,723 - +1.4 3,459,723 - -2.9 3,459,727 - -1.2 3,459,727 - -1.9 3,459,742 + -0.9
Or see this region's nucleotide sequence