Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1555 and BT1556 are separated by 73 nucleotides BT1556 and BT1557 are separated by 166 nucleotides BT1557 and BT1558 are separated by 26 nucleotides
BT1555: BT1555 - dioxygenase, related to 2-nitropropane dioxygenase (NCBI ptt file), at 1,922,759 to 1,923,694
BT1555
BT1556: BT1556 - hypothetical protein (NCBI ptt file), at 1,923,768 to 1,924,496
BT1556
BT1557: BT1557 - hypothetical protein (NCBI ptt file), at 1,924,663 to 1,925,172
BT1557
BT1558: BT1558 - hypothetical protein (NCBI ptt file), at 1,925,199 to 1,926,251
BT1558
Position (kb)
1923
1924
1925 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1923.003 kb on - strand, within BT1555 at 1923.003 kb on - strand, within BT1555 at 1923.067 kb on - strand, within BT1555 at 1923.128 kb on + strand, within BT1555 at 1923.163 kb on + strand, within BT1555 at 1923.359 kb on + strand, within BT1555 at 1923.367 kb on + strand, within BT1555 at 1923.400 kb on + strand, within BT1555 at 1923.478 kb on - strand, within BT1555 at 1923.656 kb on - strand at 1923.682 kb on + strand at 1923.718 kb on + strand at 1923.805 kb on - strand at 1923.846 kb on - strand, within BT1556 at 1923.938 kb on - strand, within BT1556 at 1923.989 kb on + strand, within BT1556 at 1924.106 kb on - strand, within BT1556 at 1924.157 kb on + strand, within BT1556 at 1924.205 kb on + strand, within BT1556 at 1924.206 kb on - strand, within BT1556 at 1924.216 kb on + strand, within BT1556 at 1924.464 kb on - strand at 1924.604 kb on + strand at 1924.899 kb on + strand, within BT1557 at 1924.937 kb on + strand, within BT1557 at 1924.960 kb on - strand, within BT1557 at 1924.980 kb on - strand, within BT1557 at 1924.987 kb on + strand, within BT1557 at 1925.204 kb on - strand at 1925.238 kb on - strand at 1925.339 kb on - strand, within BT1558 at 1925.339 kb on - strand, within BT1558 at 1925.376 kb on - strand, within BT1558 at 1925.388 kb on - strand, within BT1558 at 1925.442 kb on - strand, within BT1558 at 1925.446 kb on + strand, within BT1558 at 1925.446 kb on + strand, within BT1558 at 1925.447 kb on - strand, within BT1558
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 1,923,003 - BT1555 0.26 -1.1 1,923,003 - BT1555 0.26 -1.4 1,923,067 - BT1555 0.33 -0.1 1,923,128 + BT1555 0.39 +0.9 1,923,163 + BT1555 0.43 -1.4 1,923,359 + BT1555 0.64 -0.1 1,923,367 + BT1555 0.65 -1.6 1,923,400 + BT1555 0.68 -1.9 1,923,478 - BT1555 0.77 -0.1 1,923,656 - -0.1 1,923,682 + -0.1 1,923,718 + +1.6 1,923,805 - -2.1 1,923,846 - BT1556 0.11 -1.7 1,923,938 - BT1556 0.23 -1.6 1,923,989 + BT1556 0.30 -1.1 1,924,106 - BT1556 0.46 -1.7 1,924,157 + BT1556 0.53 -2.3 1,924,205 + BT1556 0.60 -1.4 1,924,206 - BT1556 0.60 -3.2 1,924,216 + BT1556 0.61 -0.1 1,924,464 - +0.6 1,924,604 + -1.6 1,924,899 + BT1557 0.46 -2.6 1,924,937 + BT1557 0.54 -0.9 1,924,960 - BT1557 0.58 -1.1 1,924,980 - BT1557 0.62 -2.1 1,924,987 + BT1557 0.64 +0.3 1,925,204 - +1.4 1,925,238 - +4.1 1,925,339 - BT1558 0.13 -1.1 1,925,339 - BT1558 0.13 +2.0 1,925,376 - BT1558 0.17 +3.1 1,925,388 - BT1558 0.18 -0.1 1,925,442 - BT1558 0.23 -2.4 1,925,446 + BT1558 0.23 -1.8 1,925,446 + BT1558 0.23 +2.9 1,925,447 - BT1558 0.24 +1.9
Or see this region's nucleotide sequence