Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1503 and BT1504 are separated by 281 nucleotides BT1504 and BT1505 are separated by 9 nucleotides BT1505 and BT1506 are separated by 61 nucleotides BT1506 and BT1507 are separated by 86 nucleotides
BT1503: BT1503 - integrase (NCBI ptt file), at 1,855,012 to 1,856,022
BT1503
BT1504: BT1504 - hypothetical protein (NCBI ptt file), at 1,856,304 to 1,856,567
BT1504
BT1505: BT1505 - hypothetical protein (NCBI ptt file), at 1,856,577 to 1,857,665
BT1505
BT1506: BT1506 - hypothetical protein (NCBI ptt file), at 1,857,727 to 1,858,218
BT1506
BT1507: BT1507 - hypothetical protein (NCBI ptt file), at 1,858,305 to 1,861,475
BT1507
Position (kb)
1856
1857
1858 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1855.816 kb on + strand, within BT1503 at 1855.960 kb on + strand at 1856.022 kb on + strand at 1856.024 kb on + strand at 1856.024 kb on + strand at 1856.060 kb on - strand at 1856.082 kb on + strand at 1856.108 kb on - strand at 1856.195 kb on - strand at 1856.203 kb on - strand at 1856.203 kb on - strand at 1856.203 kb on - strand at 1856.275 kb on + strand at 1856.348 kb on + strand, within BT1504 at 1856.376 kb on - strand, within BT1504 at 1856.428 kb on + strand, within BT1504 at 1856.541 kb on - strand at 1856.589 kb on - strand at 1856.594 kb on + strand at 1856.595 kb on - strand at 1856.646 kb on - strand at 1856.768 kb on - strand, within BT1505 at 1857.270 kb on + strand, within BT1505 at 1857.329 kb on + strand, within BT1505 at 1857.352 kb on - strand, within BT1505 at 1857.371 kb on + strand, within BT1505 at 1857.473 kb on + strand, within BT1505 at 1857.551 kb on + strand, within BT1505 at 1857.570 kb on + strand at 1857.593 kb on + strand at 1857.616 kb on - strand at 1857.637 kb on - strand at 1857.728 kb on + strand at 1857.728 kb on + strand at 1857.729 kb on - strand at 1857.729 kb on - strand at 1857.840 kb on + strand, within BT1506 at 1857.889 kb on - strand, within BT1506 at 1857.905 kb on + strand, within BT1506 at 1857.935 kb on + strand, within BT1506 at 1858.031 kb on - strand, within BT1506 at 1858.051 kb on - strand, within BT1506 at 1858.078 kb on + strand, within BT1506 at 1858.078 kb on + strand, within BT1506 at 1858.089 kb on + strand, within BT1506 at 1858.090 kb on - strand, within BT1506 at 1858.192 kb on + strand at 1858.194 kb on + strand at 1858.194 kb on + strand at 1858.198 kb on + strand at 1858.289 kb on - strand at 1858.319 kb on + strand at 1858.319 kb on + strand at 1858.332 kb on - strand at 1858.364 kb on + strand at 1858.463 kb on - strand at 1858.542 kb on - strand at 1858.542 kb on - strand at 1858.542 kb on - strand at 1858.596 kb on + strand at 1858.633 kb on - strand, within BT1507 at 1858.645 kb on - strand, within BT1507
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 1,855,816 + BT1503 0.80 -1.4 1,855,960 + +1.5 1,856,022 + -0.1 1,856,024 + -2.1 1,856,024 + +1.5 1,856,060 - +2.5 1,856,082 + +2.7 1,856,108 - -2.1 1,856,195 - -0.1 1,856,203 - +1.4 1,856,203 - -0.7 1,856,203 - -1.1 1,856,275 + +2.5 1,856,348 + BT1504 0.17 -1.6 1,856,376 - BT1504 0.27 -2.2 1,856,428 + BT1504 0.47 +2.5 1,856,541 - +0.9 1,856,589 - +2.7 1,856,594 + +3.0 1,856,595 - -0.1 1,856,646 - -1.3 1,856,768 - BT1505 0.18 -2.7 1,857,270 + BT1505 0.64 -1.5 1,857,329 + BT1505 0.69 +1.9 1,857,352 - BT1505 0.71 -0.1 1,857,371 + BT1505 0.73 +1.1 1,857,473 + BT1505 0.82 -0.6 1,857,551 + BT1505 0.89 +2.0 1,857,570 + +3.4 1,857,593 + +0.3 1,857,616 - +1.6 1,857,637 - -0.4 1,857,728 + -0.9 1,857,728 + +2.7 1,857,729 - -2.1 1,857,729 - -1.1 1,857,840 + BT1506 0.23 -0.5 1,857,889 - BT1506 0.33 +1.5 1,857,905 + BT1506 0.36 -1.1 1,857,935 + BT1506 0.42 -3.1 1,858,031 - BT1506 0.62 -1.6 1,858,051 - BT1506 0.66 -1.1 1,858,078 + BT1506 0.71 +2.7 1,858,078 + BT1506 0.71 +1.5 1,858,089 + BT1506 0.74 -1.5 1,858,090 - BT1506 0.74 -0.7 1,858,192 + -3.4 1,858,194 + -0.1 1,858,194 + -2.1 1,858,198 + +2.5 1,858,289 - -0.1 1,858,319 + -0.7 1,858,319 + -1.7 1,858,332 - -3.3 1,858,364 + -2.1 1,858,463 - -2.5 1,858,542 - -2.1 1,858,542 - -3.3 1,858,542 - -1.7 1,858,596 + -1.7 1,858,633 - BT1507 0.10 +0.2 1,858,645 - BT1507 0.11 -2.4
Or see this region's nucleotide sequence