Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0782 and BT0783 overlap by 52 nucleotides BT0783 and BT0784 are separated by 278 nucleotides BT0784 and BT0785 are separated by 181 nucleotides BT0785 and BT0786 are separated by 390 nucleotides
BT0782: BT0782 - hypothetical protein (NCBI ptt file), at 972,573 to 972,776
BT0782
BT0783: BT0783 - hypothetical protein (NCBI ptt file), at 972,725 to 972,913
BT0783
BT0784: BT0784 - putative RNA-binding protein (NCBI ptt file), at 973,192 to 973,437
BT0784
BT0785: BT0785 - glutamine synthetase I (NCBI ptt file), at 973,619 to 975,121
BT0785
BT0786: BT0786 - putative integral membrane protein (NCBI ptt file), at 975,512 to 976,234
BT0786
Position (kb) 973
974
975
976 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 972.667 kb on - strand, within BT0782 at 972.704 kb on + strand, within BT0782 at 972.742 kb on + strand at 972.743 kb on - strand at 972.743 kb on - strand at 972.767 kb on + strand at 972.786 kb on - strand, within BT0783 at 972.856 kb on + strand, within BT0783 at 972.857 kb on - strand, within BT0783 at 972.914 kb on + strand at 972.956 kb on + strand at 973.135 kb on + strand at 973.219 kb on + strand, within BT0784 at 973.221 kb on + strand, within BT0784 at 973.457 kb on + strand at 973.461 kb on + strand at 973.723 kb on + strand at 973.822 kb on + strand, within BT0785 at 973.865 kb on - strand, within BT0785 at 973.889 kb on + strand, within BT0785 at 973.893 kb on + strand, within BT0785 at 973.894 kb on - strand, within BT0785 at 973.894 kb on - strand, within BT0785 at 974.035 kb on + strand, within BT0785 at 974.036 kb on - strand, within BT0785 at 974.050 kb on + strand, within BT0785 at 974.081 kb on + strand, within BT0785 at 974.186 kb on - strand, within BT0785 at 974.186 kb on - strand, within BT0785 at 974.192 kb on + strand, within BT0785 at 974.192 kb on + strand, within BT0785 at 974.193 kb on - strand, within BT0785 at 974.194 kb on + strand, within BT0785 at 974.196 kb on + strand, within BT0785 at 974.197 kb on - strand, within BT0785 at 974.268 kb on - strand, within BT0785 at 974.268 kb on - strand, within BT0785 at 974.361 kb on - strand, within BT0785 at 974.372 kb on + strand, within BT0785 at 974.373 kb on - strand, within BT0785 at 974.374 kb on + strand, within BT0785 at 974.374 kb on + strand, within BT0785 at 974.375 kb on - strand, within BT0785 at 974.441 kb on - strand, within BT0785 at 974.537 kb on - strand, within BT0785 at 974.537 kb on - strand, within BT0785 at 974.537 kb on - strand, within BT0785 at 974.650 kb on - strand, within BT0785 at 974.683 kb on + strand, within BT0785 at 974.683 kb on + strand, within BT0785 at 974.859 kb on - strand, within BT0785 at 975.097 kb on - strand at 975.127 kb on - strand at 975.153 kb on - strand at 975.230 kb on + strand at 975.284 kb on + strand at 975.342 kb on - strand at 975.462 kb on - strand at 975.511 kb on + strand at 975.522 kb on + strand at 975.661 kb on - strand, within BT0786 at 975.669 kb on - strand, within BT0786 at 975.773 kb on - strand, within BT0786 at 975.853 kb on - strand, within BT0786 at 975.856 kb on - strand, within BT0786 at 975.872 kb on - strand, within BT0786 at 975.875 kb on + strand, within BT0786 at 975.907 kb on + strand, within BT0786 at 975.908 kb on - strand, within BT0786 at 975.932 kb on - strand, within BT0786 at 975.969 kb on - strand, within BT0786 at 976.068 kb on - strand, within BT0786
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 972,667 - BT0782 0.46 +0.6 972,704 + BT0782 0.64 +2.5 972,742 + -0.3 972,743 - -0.5 972,743 - -0.5 972,767 + -2.6 972,786 - BT0783 0.32 -1.1 972,856 + BT0783 0.69 -2.3 972,857 - BT0783 0.70 +3.7 972,914 + -2.5 972,956 + -1.5 973,135 + -3.7 973,219 + BT0784 0.11 -0.5 973,221 + BT0784 0.12 -4.2 973,457 + +0.1 973,461 + -1.8 973,723 + -1.2 973,822 + BT0785 0.14 +0.7 973,865 - BT0785 0.16 +1.5 973,889 + BT0785 0.18 -2.5 973,893 + BT0785 0.18 -0.2 973,894 - BT0785 0.18 -2.6 973,894 - BT0785 0.18 +1.8 974,035 + BT0785 0.28 -1.1 974,036 - BT0785 0.28 +0.7 974,050 + BT0785 0.29 -1.5 974,081 + BT0785 0.31 -1.5 974,186 - BT0785 0.38 +0.5 974,186 - BT0785 0.38 -2.5 974,192 + BT0785 0.38 -1.6 974,192 + BT0785 0.38 +1.8 974,193 - BT0785 0.38 +0.5 974,194 + BT0785 0.38 +2.1 974,196 + BT0785 0.38 +2.4 974,197 - BT0785 0.38 -3.1 974,268 - BT0785 0.43 -2.5 974,268 - BT0785 0.43 -0.5 974,361 - BT0785 0.49 -0.3 974,372 + BT0785 0.50 +0.5 974,373 - BT0785 0.50 +2.0 974,374 + BT0785 0.50 +0.9 974,374 + BT0785 0.50 +0.5 974,375 - BT0785 0.50 -0.5 974,441 - BT0785 0.55 +0.8 974,537 - BT0785 0.61 +0.5 974,537 - BT0785 0.61 +1.5 974,537 - BT0785 0.61 -2.1 974,650 - BT0785 0.69 +0.1 974,683 + BT0785 0.71 -0.5 974,683 + BT0785 0.71 -1.3 974,859 - BT0785 0.83 +2.5 975,097 - -1.5 975,127 - +1.7 975,153 - -2.2 975,230 + +2.1 975,284 + -0.5 975,342 - -4.4 975,462 - +0.1 975,511 + +1.6 975,522 + +0.5 975,661 - BT0786 0.21 -3.1 975,669 - BT0786 0.22 -2.0 975,773 - BT0786 0.36 +0.3 975,853 - BT0786 0.47 +3.2 975,856 - BT0786 0.48 -2.5 975,872 - BT0786 0.50 -0.5 975,875 + BT0786 0.50 -1.1 975,907 + BT0786 0.55 -0.0 975,908 - BT0786 0.55 +0.4 975,932 - BT0786 0.58 -0.5 975,969 - BT0786 0.63 +0.1 976,068 - BT0786 0.77 +1.1
Or see this region's nucleotide sequence