Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0234 and BT0235 are separated by 457 nucleotides BT0235 and BT0236 are separated by 268 nucleotides
BT0234: BT0234 - putative transposase (NCBI ptt file), at 254,974 to 255,924
BT0234
BT0235: BT0235 - conserved hypothetical protein (NCBI ptt file), at 256,382 to 257,509
BT0235
BT0236: BT0236 - conserved hypothetical protein (NCBI ptt file), at 257,778 to 259,826
BT0236
Position (kb) 256
257
258 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 255.428 kb on + strand, within BT0234 at 255.623 kb on - strand, within BT0234 at 255.703 kb on - strand, within BT0234 at 255.704 kb on + strand, within BT0234 at 255.705 kb on - strand, within BT0234 at 255.725 kb on - strand, within BT0234 at 255.747 kb on + strand, within BT0234 at 255.753 kb on - strand, within BT0234 at 255.925 kb on - strand at 255.940 kb on + strand at 255.940 kb on + strand at 256.241 kb on - strand at 256.288 kb on - strand at 256.307 kb on + strand at 256.436 kb on - strand at 256.539 kb on + strand, within BT0235 at 256.651 kb on - strand, within BT0235 at 256.660 kb on + strand, within BT0235 at 256.685 kb on - strand, within BT0235 at 256.718 kb on + strand, within BT0235 at 256.743 kb on - strand, within BT0235 at 256.770 kb on - strand, within BT0235 at 256.770 kb on - strand, within BT0235 at 256.801 kb on - strand, within BT0235 at 256.880 kb on - strand, within BT0235 at 257.015 kb on + strand, within BT0235 at 257.016 kb on - strand, within BT0235 at 257.018 kb on - strand, within BT0235 at 257.064 kb on + strand, within BT0235 at 257.257 kb on - strand, within BT0235 at 257.291 kb on - strand, within BT0235 at 257.375 kb on + strand, within BT0235 at 257.376 kb on - strand at 257.376 kb on - strand, within BT0235 at 257.495 kb on + strand at 257.527 kb on + strand at 257.561 kb on - strand at 257.665 kb on + strand at 257.665 kb on + strand at 257.666 kb on - strand at 257.667 kb on + strand at 257.672 kb on + strand at 257.673 kb on - strand at 257.802 kb on - strand at 257.822 kb on - strand at 257.838 kb on + strand at 257.839 kb on - strand at 257.843 kb on + strand at 257.844 kb on - strand at 257.857 kb on - strand at 257.941 kb on - strand at 257.941 kb on - strand at 257.988 kb on - strand, within BT0236 at 257.997 kb on - strand, within BT0236 at 258.062 kb on - strand, within BT0236 at 258.062 kb on - strand, within BT0236 at 258.123 kb on - strand, within BT0236 at 258.276 kb on + strand, within BT0236 at 258.276 kb on + strand, within BT0236 at 258.282 kb on - strand, within BT0236 at 258.384 kb on + strand, within BT0236 at 258.385 kb on - strand, within BT0236 at 258.385 kb on - strand, within BT0236 at 258.389 kb on + strand, within BT0236 at 258.394 kb on - strand, within BT0236 at 258.465 kb on + strand, within BT0236 at 258.477 kb on + strand at 258.480 kb on - strand, within BT0236 at 258.501 kb on + strand, within BT0236 at 258.502 kb on - strand, within BT0236
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 255,428 + BT0234 0.48 -2.5 255,623 - BT0234 0.68 -1.7 255,703 - BT0234 0.77 -1.9 255,704 + BT0234 0.77 -1.0 255,705 - BT0234 0.77 -0.1 255,725 - BT0234 0.79 -0.1 255,747 + BT0234 0.81 -1.7 255,753 - BT0234 0.82 -1.7 255,925 - -2.8 255,940 + +2.4 255,940 + +1.4 256,241 - -1.7 256,288 - -2.9 256,307 + -3.1 256,436 - -1.7 256,539 + BT0235 0.14 -2.7 256,651 - BT0235 0.24 -1.1 256,660 + BT0235 0.25 -1.3 256,685 - BT0235 0.27 -1.1 256,718 + BT0235 0.30 -0.6 256,743 - BT0235 0.32 -3.8 256,770 - BT0235 0.34 -1.7 256,770 - BT0235 0.34 -2.7 256,801 - BT0235 0.37 -1.1 256,880 - BT0235 0.44 -2.3 257,015 + BT0235 0.56 +0.9 257,016 - BT0235 0.56 -1.4 257,018 - BT0235 0.56 -2.7 257,064 + BT0235 0.60 +1.2 257,257 - BT0235 0.78 +2.7 257,291 - BT0235 0.81 -0.1 257,375 + BT0235 0.88 -0.5 257,376 - -1.1 257,376 - BT0235 0.88 +1.1 257,495 + -2.1 257,527 + -2.1 257,561 - +0.4 257,665 + +2.9 257,665 + +3.7 257,666 - -2.1 257,667 + +0.9 257,672 + -0.1 257,673 - +2.3 257,802 - -1.0 257,822 - -1.7 257,838 + -2.1 257,839 - +0.1 257,843 + -3.9 257,844 - -1.3 257,857 - -2.5 257,941 - -1.1 257,941 - -2.1 257,988 - BT0236 0.10 -1.1 257,997 - BT0236 0.11 -0.5 258,062 - BT0236 0.14 -3.1 258,062 - BT0236 0.14 -0.1 258,123 - BT0236 0.17 +0.4 258,276 + BT0236 0.24 +0.8 258,276 + BT0236 0.24 +1.9 258,282 - BT0236 0.25 -2.1 258,384 + BT0236 0.30 +0.4 258,385 - BT0236 0.30 -0.1 258,385 - BT0236 0.30 -1.7 258,389 + BT0236 0.30 +0.4 258,394 - BT0236 0.30 -1.7 258,465 + BT0236 0.34 -0.6 258,477 + -2.1 258,480 - BT0236 0.34 -1.5 258,501 + BT0236 0.35 +2.3 258,502 - BT0236 0.35 +0.7
Or see this region's nucleotide sequence