Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0183 and BT0184 are separated by 17 nucleotides BT0184 and BT0185 are separated by 101 nucleotides BT0185 and BT0186 are separated by 9 nucleotides
BT0183: BT0183 - transglycosylase (NCBI ptt file), at 192,751 to 194,139
BT0183
BT0184: BT0184 - uridine kinase (uridine monophosphokinase) (NCBI ptt file), at 194,157 to 194,774
BT0184
BT0185: BT0185 - conserved hypothetical protein (NCBI ptt file), at 194,876 to 195,175
BT0185
BT0186: BT0186 - thiol:disulfide interchange protein dsbD precursor (NCBI ptt file), at 195,185 to 197,245
BT0186
Position (kb) 194
195
196 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 193.980 kb on + strand, within BT0183 at 193.986 kb on + strand, within BT0183 at 193.986 kb on + strand, within BT0183 at 193.987 kb on - strand, within BT0183 at 193.988 kb on + strand, within BT0183 at 193.989 kb on - strand, within BT0183 at 193.989 kb on - strand, within BT0183 at 194.087 kb on - strand at 194.102 kb on - strand at 194.103 kb on + strand at 194.189 kb on - strand at 194.418 kb on - strand, within BT0184 at 194.451 kb on - strand, within BT0184 at 194.451 kb on - strand, within BT0184 at 194.524 kb on + strand, within BT0184 at 194.648 kb on + strand at 194.680 kb on - strand, within BT0184 at 194.683 kb on - strand, within BT0184 at 194.686 kb on - strand, within BT0184 at 194.766 kb on + strand at 194.820 kb on + strand at 194.845 kb on + strand at 194.874 kb on + strand at 194.899 kb on + strand at 195.089 kb on + strand, within BT0185 at 195.130 kb on + strand, within BT0185
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 193,980 + BT0183 0.88 -0.1 193,986 + BT0183 0.89 -0.7 193,986 + BT0183 0.89 +2.3 193,987 - BT0183 0.89 +2.0 193,988 + BT0183 0.89 +2.2 193,989 - BT0183 0.89 +2.9 193,989 - BT0183 0.89 +0.9 194,087 - +0.4 194,102 - +1.9 194,103 + +1.9 194,189 - -2.9 194,418 - BT0184 0.42 -2.0 194,451 - BT0184 0.48 -0.1 194,451 - BT0184 0.48 -2.7 194,524 + BT0184 0.59 +0.9 194,648 + +0.9 194,680 - BT0184 0.85 -2.1 194,683 - BT0184 0.85 -1.1 194,686 - BT0184 0.86 -1.5 194,766 + -0.1 194,820 + -0.9 194,845 + +0.9 194,874 + +1.9 194,899 + -0.1 195,089 + BT0185 0.71 +2.2 195,130 + BT0185 0.85 +1.9
Or see this region's nucleotide sequence