Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0177 and BT0178 are separated by 26 nucleotides BT0178 and BT0179 are separated by 9 nucleotides BT0179 and BT0180 are separated by 89 nucleotides
BT0177: BT0177 - hypothetical protein (NCBI ptt file), at 185,638 to 186,429
BT0177
BT0178: BT0178 - hypothetical protein (NCBI ptt file), at 186,456 to 187,001
BT0178
BT0179: BT0179 - SsrA-binding protein (NCBI ptt file), at 187,011 to 187,463
BT0179
BT0180: BT0180 - 5-methyltetrahydrofolate-homocysteine methyltransferase (NCBI ptt file), at 187,553 to 190,300
BT0180
Position (kb) 187
188 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 186.016 kb on + strand, within BT0177 at 186.033 kb on + strand, within BT0177 at 186.088 kb on + strand, within BT0177 at 186.108 kb on + strand, within BT0177 at 186.160 kb on + strand, within BT0177 at 186.160 kb on + strand, within BT0177 at 186.318 kb on + strand, within BT0177 at 186.449 kb on + strand at 186.451 kb on + strand at 186.545 kb on + strand, within BT0178 at 186.625 kb on + strand, within BT0178 at 186.695 kb on + strand, within BT0178 at 186.855 kb on + strand, within BT0178 at 186.943 kb on + strand, within BT0178 at 186.946 kb on + strand, within BT0178 at 186.946 kb on + strand, within BT0178 at 186.991 kb on + strand at 186.999 kb on + strand at 187.480 kb on - strand at 187.502 kb on - strand at 187.512 kb on + strand at 187.540 kb on + strand at 187.541 kb on - strand at 187.632 kb on - strand at 187.632 kb on - strand at 187.676 kb on - strand at 187.742 kb on - strand at 187.789 kb on + strand at 187.838 kb on + strand, within BT0180 at 187.895 kb on + strand, within BT0180 at 187.948 kb on + strand, within BT0180 at 187.949 kb on - strand, within BT0180 at 188.141 kb on - strand, within BT0180 at 188.157 kb on + strand, within BT0180 at 188.157 kb on + strand, within BT0180 at 188.158 kb on - strand, within BT0180 at 188.207 kb on - strand, within BT0180 at 188.207 kb on - strand, within BT0180 at 188.261 kb on - strand, within BT0180 at 188.345 kb on + strand, within BT0180 at 188.362 kb on + strand, within BT0180 at 188.363 kb on - strand, within BT0180 at 188.363 kb on - strand, within BT0180 at 188.438 kb on - strand, within BT0180
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 186,016 + BT0177 0.48 -1.7 186,033 + BT0177 0.50 -1.5 186,088 + BT0177 0.57 -0.1 186,108 + BT0177 0.59 -0.1 186,160 + BT0177 0.66 -0.5 186,160 + BT0177 0.66 -1.5 186,318 + BT0177 0.86 -1.4 186,449 + -2.5 186,451 + -2.9 186,545 + BT0178 0.16 +1.7 186,625 + BT0178 0.31 +3.0 186,695 + BT0178 0.44 +3.4 186,855 + BT0178 0.73 +0.9 186,943 + BT0178 0.89 +1.6 186,946 + BT0178 0.90 -1.6 186,946 + BT0178 0.90 +0.9 186,991 + +2.2 186,999 + -0.1 187,480 - -3.1 187,502 - -3.9 187,512 + -1.1 187,540 + -2.0 187,541 - -3.0 187,632 - -3.7 187,632 - -1.7 187,676 - +1.4 187,742 - -2.5 187,789 + -1.1 187,838 + BT0180 0.10 +1.4 187,895 + BT0180 0.12 -0.7 187,948 + BT0180 0.14 -0.1 187,949 - BT0180 0.14 +0.1 188,141 - BT0180 0.21 -1.0 188,157 + BT0180 0.22 +2.3 188,157 + BT0180 0.22 -2.4 188,158 - BT0180 0.22 +0.7 188,207 - BT0180 0.24 -1.3 188,207 - BT0180 0.24 -3.5 188,261 - BT0180 0.26 -2.2 188,345 + BT0180 0.29 -1.7 188,362 + BT0180 0.29 -1.7 188,363 - BT0180 0.29 -4.0 188,363 - BT0180 0.29 -1.5 188,438 - BT0180 0.32 -2.7
Or see this region's nucleotide sequence