Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0177

Experiment: BHIS with 5-Fluorouracil 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0176 and BT0177 are separated by 76 nucleotidesBT0177 and BT0178 are separated by 26 nucleotidesBT0178 and BT0179 are separated by 9 nucleotides BT0176: BT0176 - conserved hypothetical protein (NCBI ptt file), at 185,073 to 185,561 BT0176 BT0177: BT0177 - hypothetical protein (NCBI ptt file), at 185,638 to 186,429 BT0177 BT0178: BT0178 - hypothetical protein (NCBI ptt file), at 186,456 to 187,001 BT0178 BT0179: BT0179 - SsrA-binding protein (NCBI ptt file), at 187,011 to 187,463 BT0179 Position (kb) 185 186 187Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 184.661 kb on + strandat 184.712 kb on - strandat 184.792 kb on - strandat 184.792 kb on - strandat 184.792 kb on - strandat 184.811 kb on - strandat 184.815 kb on + strandat 184.832 kb on + strandat 184.884 kb on - strandat 185.006 kb on - strandat 185.006 kb on - strandat 185.010 kb on - strandat 185.013 kb on + strandat 185.013 kb on + strandat 185.016 kb on - strandat 185.030 kb on - strandat 185.048 kb on - strandat 185.050 kb on - strandat 185.061 kb on - strandat 185.121 kb on - strandat 185.138 kb on + strand, within BT0176at 185.138 kb on + strand, within BT0176at 185.139 kb on - strand, within BT0176at 185.142 kb on + strand, within BT0176at 185.153 kb on - strand, within BT0176at 185.220 kb on + strand, within BT0176at 185.242 kb on + strand, within BT0176at 185.243 kb on - strand, within BT0176at 185.290 kb on - strand, within BT0176at 185.291 kb on + strand, within BT0176at 185.291 kb on + strand, within BT0176at 185.292 kb on - strand, within BT0176at 185.292 kb on - strand, within BT0176at 185.292 kb on - strand, within BT0176at 185.292 kb on - strand, within BT0176at 185.292 kb on - strand, within BT0176at 185.349 kb on - strand, within BT0176at 185.356 kb on + strand, within BT0176at 185.358 kb on + strand, within BT0176at 185.360 kb on + strand, within BT0176at 185.360 kb on + strand, within BT0176at 185.361 kb on - strand, within BT0176at 185.361 kb on - strand, within BT0176at 185.374 kb on + strand, within BT0176at 185.375 kb on - strand, within BT0176at 185.375 kb on - strand, within BT0176at 185.376 kb on + strand, within BT0176at 185.377 kb on - strand, within BT0176at 185.436 kb on + strand, within BT0176at 185.436 kb on + strand, within BT0176at 185.437 kb on - strand, within BT0176at 185.445 kb on + strand, within BT0176at 185.446 kb on - strand, within BT0176at 185.446 kb on - strand, within BT0176at 185.446 kb on - strand, within BT0176at 185.446 kb on - strand, within BT0176at 185.476 kb on - strand, within BT0176at 185.479 kb on + strandat 185.479 kb on + strand, within BT0176at 185.479 kb on + strand, within BT0176at 185.479 kb on + strand, within BT0176at 185.479 kb on + strand, within BT0176at 185.480 kb on - strand, within BT0176at 185.480 kb on - strand, within BT0176at 185.480 kb on - strand, within BT0176at 185.484 kb on - strand, within BT0176at 185.517 kb on + strandat 185.517 kb on + strandat 185.518 kb on - strandat 185.521 kb on + strandat 185.522 kb on - strandat 185.525 kb on + strandat 185.526 kb on - strandat 185.526 kb on - strandat 185.554 kb on + strandat 185.630 kb on + strandat 185.727 kb on + strand, within BT0177at 185.770 kb on + strand, within BT0177at 185.918 kb on + strand, within BT0177at 185.949 kb on + strand, within BT0177at 186.016 kb on + strand, within BT0177at 186.033 kb on + strand, within BT0177at 186.088 kb on + strand, within BT0177at 186.108 kb on + strand, within BT0177at 186.160 kb on + strand, within BT0177at 186.160 kb on + strand, within BT0177at 186.318 kb on + strand, within BT0177at 186.449 kb on + strandat 186.451 kb on + strandat 186.545 kb on + strand, within BT0178at 186.625 kb on + strand, within BT0178at 186.695 kb on + strand, within BT0178at 186.855 kb on + strand, within BT0178at 186.943 kb on + strand, within BT0178at 186.946 kb on + strand, within BT0178at 186.946 kb on + strand, within BT0178at 186.991 kb on + strandat 186.999 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM
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184,661 + -2.7
184,712 - +1.6
184,792 - -1.7
184,792 - -1.2
184,792 - +0.6
184,811 - -1.1
184,815 + +0.7
184,832 + -1.4
184,884 - -2.1
185,006 - -1.0
185,006 - -2.9
185,010 - +0.1
185,013 + -2.5
185,013 + -1.5
185,016 - -1.1
185,030 - -1.9
185,048 - -3.0
185,050 - -4.3
185,061 - -2.7
185,121 - +1.9
185,138 + BT0176 0.13 -3.8
185,138 + BT0176 0.13 -3.9
185,139 - BT0176 0.13 -2.8
185,142 + BT0176 0.14 -2.9
185,153 - BT0176 0.16 -5.3
185,220 + BT0176 0.30 -0.8
185,242 + BT0176 0.35 -4.0
185,243 - BT0176 0.35 -4.3
185,290 - BT0176 0.44 +2.4
185,291 + BT0176 0.45 -3.6
185,291 + BT0176 0.45 +0.1
185,292 - BT0176 0.45 -3.4
185,292 - BT0176 0.45 -2.5
185,292 - BT0176 0.45 -1.2
185,292 - BT0176 0.45 +1.1
185,292 - BT0176 0.45 +1.5
185,349 - BT0176 0.56 +1.5
185,356 + BT0176 0.58 -2.0
185,358 + BT0176 0.58 -3.3
185,360 + BT0176 0.59 -3.1
185,360 + BT0176 0.59 +1.0
185,361 - BT0176 0.59 -1.4
185,361 - BT0176 0.59 -2.1
185,374 + BT0176 0.62 -2.7
185,375 - BT0176 0.62 -3.5
185,375 - BT0176 0.62 -3.4
185,376 + BT0176 0.62 -3.2
185,377 - BT0176 0.62 -2.9
185,436 + BT0176 0.74 +2.5
185,436 + BT0176 0.74 -3.7
185,437 - BT0176 0.74 -2.9
185,445 + BT0176 0.76 +1.8
185,446 - BT0176 0.76 -0.4
185,446 - BT0176 0.76 -2.0
185,446 - BT0176 0.76 +0.0
185,446 - BT0176 0.76 +2.0
185,476 - BT0176 0.82 -1.8
185,479 + -0.1
185,479 + BT0176 0.83 +0.7
185,479 + BT0176 0.83 -3.5
185,479 + BT0176 0.83 -3.5
185,479 + BT0176 0.83 +0.5
185,480 - BT0176 0.83 -2.7
185,480 - BT0176 0.83 +1.7
185,480 - BT0176 0.83 -5.4
185,484 - BT0176 0.84 +1.8
185,517 + -2.7
185,517 + -1.9
185,518 - -4.3
185,521 + -3.3
185,522 - +2.2
185,525 + -3.5
185,526 - +0.8
185,526 - -1.8
185,554 + -0.1
185,630 + +1.5
185,727 + BT0177 0.11 -2.5
185,770 + BT0177 0.17 +3.0
185,918 + BT0177 0.35 -0.1
185,949 + BT0177 0.39 +1.3
186,016 + BT0177 0.48 -1.7
186,033 + BT0177 0.50 -1.5
186,088 + BT0177 0.57 -0.1
186,108 + BT0177 0.59 -0.1
186,160 + BT0177 0.66 -0.5
186,160 + BT0177 0.66 -1.5
186,318 + BT0177 0.86 -1.4
186,449 + -2.5
186,451 + -2.9
186,545 + BT0178 0.16 +1.7
186,625 + BT0178 0.31 +3.0
186,695 + BT0178 0.44 +3.4
186,855 + BT0178 0.73 +0.9
186,943 + BT0178 0.89 +1.6
186,946 + BT0178 0.90 -1.6
186,946 + BT0178 0.90 +0.9
186,991 + +2.2
186,999 + -0.1

Or see this region's nucleotide sequence