Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0049 and BT0050 are separated by 11 nucleotides BT0050 and BT0051 overlap by 1 nucleotides BT0051 and BT0052 are separated by 170 nucleotides
BT0049: BT0049 - putative glycosyltransferase (NCBI ptt file), at 44,647 to 45,582
BT0049
BT0050: BT0050 - putative glycosyltransferase (NCBI ptt file), at 45,594 to 46,565
BT0050
BT0051: BT0051 - putative glycosyltransferase (NCBI ptt file), at 46,565 to 47,545
BT0051
BT0052: BT0052 - hypothetical protein (NCBI ptt file), at 47,716 to 48,834
BT0052
Position (kb) 46
47
48 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 45.567 kb on - strand at 45.630 kb on + strand at 45.661 kb on + strand at 45.703 kb on + strand, within BT0050 at 45.731 kb on + strand, within BT0050 at 45.742 kb on + strand, within BT0050 at 45.743 kb on - strand, within BT0050 at 45.943 kb on - strand, within BT0050 at 45.981 kb on + strand, within BT0050 at 46.014 kb on - strand, within BT0050 at 46.162 kb on - strand, within BT0050 at 46.213 kb on - strand, within BT0050 at 46.218 kb on - strand, within BT0050 at 46.364 kb on + strand, within BT0050 at 46.364 kb on + strand, within BT0050 at 46.365 kb on - strand, within BT0050 at 46.365 kb on - strand, within BT0050 at 46.366 kb on + strand, within BT0050 at 46.367 kb on - strand, within BT0050 at 46.382 kb on + strand, within BT0050 at 46.459 kb on - strand, within BT0050 at 46.484 kb on + strand at 46.558 kb on - strand at 46.560 kb on + strand at 46.593 kb on - strand at 46.593 kb on - strand at 46.593 kb on - strand at 46.600 kb on - strand at 46.647 kb on - strand at 46.662 kb on - strand at 46.662 kb on - strand at 46.672 kb on - strand, within BT0051 at 46.698 kb on + strand, within BT0051 at 46.755 kb on + strand, within BT0051 at 46.826 kb on + strand, within BT0051 at 46.916 kb on - strand, within BT0051 at 47.011 kb on - strand, within BT0051 at 47.042 kb on + strand, within BT0051 at 47.043 kb on - strand, within BT0051 at 47.064 kb on - strand, within BT0051 at 47.068 kb on - strand, within BT0051 at 47.103 kb on + strand, within BT0051 at 47.122 kb on - strand, within BT0051 at 47.416 kb on + strand, within BT0051 at 47.512 kb on - strand at 47.559 kb on + strand at 47.565 kb on - strand at 47.565 kb on - strand at 47.565 kb on - strand at 47.592 kb on + strand at 47.631 kb on + strand at 47.635 kb on - strand at 47.642 kb on - strand at 47.642 kb on - strand at 47.794 kb on - strand at 47.800 kb on + strand at 47.800 kb on + strand at 47.809 kb on + strand at 47.809 kb on + strand at 47.810 kb on - strand at 47.810 kb on - strand at 47.816 kb on + strand at 47.817 kb on - strand at 47.817 kb on - strand at 47.862 kb on + strand, within BT0052 at 47.863 kb on - strand, within BT0052 at 47.909 kb on - strand, within BT0052 at 47.909 kb on - strand, within BT0052 at 47.992 kb on + strand, within BT0052 at 48.037 kb on + strand, within BT0052 at 48.038 kb on - strand, within BT0052 at 48.038 kb on - strand, within BT0052 at 48.038 kb on - strand, within BT0052 at 48.099 kb on - strand, within BT0052 at 48.152 kb on + strand, within BT0052 at 48.177 kb on + strand, within BT0052 at 48.186 kb on + strand, within BT0052 at 48.189 kb on + strand, within BT0052 at 48.190 kb on - strand, within BT0052 at 48.222 kb on - strand, within BT0052 at 48.242 kb on + strand, within BT0052 at 48.242 kb on + strand, within BT0052 at 48.243 kb on - strand, within BT0052 at 48.324 kb on + strand, within BT0052 at 48.325 kb on - strand, within BT0052 at 48.366 kb on + strand, within BT0052 at 48.414 kb on + strand, within BT0052 at 48.476 kb on + strand, within BT0052 at 48.477 kb on - strand, within BT0052 at 48.502 kb on + strand, within BT0052
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 45,567 - -3.0 45,630 + +1.6 45,661 + +0.7 45,703 + BT0050 0.11 +3.2 45,731 + BT0050 0.14 +1.3 45,742 + BT0050 0.15 -0.1 45,743 - BT0050 0.15 -2.6 45,943 - BT0050 0.36 -2.4 45,981 + BT0050 0.40 -1.4 46,014 - BT0050 0.43 -4.2 46,162 - BT0050 0.58 -1.3 46,213 - BT0050 0.64 +2.7 46,218 - BT0050 0.64 -2.1 46,364 + BT0050 0.79 +0.9 46,364 + BT0050 0.79 -3.3 46,365 - BT0050 0.79 -1.1 46,365 - BT0050 0.79 +0.3 46,366 + BT0050 0.79 -2.0 46,367 - BT0050 0.80 -0.3 46,382 + BT0050 0.81 -1.3 46,459 - BT0050 0.89 -3.0 46,484 + -2.3 46,558 - +0.5 46,560 + -0.1 46,593 - -2.1 46,593 - -1.4 46,593 - +0.5 46,600 - -0.1 46,647 - -2.4 46,662 - +0.1 46,662 - -0.4 46,672 - BT0051 0.11 +2.7 46,698 + BT0051 0.14 +1.0 46,755 + BT0051 0.19 +1.5 46,826 + BT0051 0.27 -2.1 46,916 - BT0051 0.36 -0.4 47,011 - BT0051 0.45 -0.1 47,042 + BT0051 0.49 +1.9 47,043 - BT0051 0.49 +1.2 47,064 - BT0051 0.51 +1.8 47,068 - BT0051 0.51 -0.9 47,103 + BT0051 0.55 -0.5 47,122 - BT0051 0.57 +4.0 47,416 + BT0051 0.87 -2.1 47,512 - -2.5 47,559 + -1.4 47,565 - -1.3 47,565 - -1.2 47,565 - +1.5 47,592 + -1.0 47,631 + -1.2 47,635 - -1.7 47,642 - +0.1 47,642 - -0.5 47,794 - -2.9 47,800 + +0.9 47,800 + +0.9 47,809 + -2.1 47,809 + -1.8 47,810 - -0.9 47,810 - -1.4 47,816 + -3.2 47,817 - -1.4 47,817 - -2.1 47,862 + BT0052 0.13 -2.0 47,863 - BT0052 0.13 +0.9 47,909 - BT0052 0.17 +0.7 47,909 - BT0052 0.17 -1.1 47,992 + BT0052 0.25 +2.9 48,037 + BT0052 0.29 -2.1 48,038 - BT0052 0.29 -0.1 48,038 - BT0052 0.29 -2.4 48,038 - BT0052 0.29 -0.1 48,099 - BT0052 0.34 -0.4 48,152 + BT0052 0.39 -0.8 48,177 + BT0052 0.41 +1.9 48,186 + BT0052 0.42 +0.1 48,189 + BT0052 0.42 -1.1 48,190 - BT0052 0.42 -0.7 48,222 - BT0052 0.45 +0.9 48,242 + BT0052 0.47 -0.1 48,242 + BT0052 0.47 +1.2 48,243 - BT0052 0.47 -0.1 48,324 + BT0052 0.54 -3.1 48,325 - BT0052 0.54 -2.3 48,366 + BT0052 0.58 +2.7 48,414 + BT0052 0.62 +1.1 48,476 + BT0052 0.68 +0.9 48,477 - BT0052 0.68 -3.3 48,502 + BT0052 0.70 -0.1
Or see this region's nucleotide sequence