Experiment: BHIS with 5-Fluorouracil 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt p5482_14 and p5482_15 are separated by 4 nucleotides p5482_15 and p5482_16 overlap by 4 nucleotides p5482_16 and p5482_17 are separated by 536 nucleotides p5482_17 and p5482_18 are separated by 10 nucleotides
p5482_14: p5482_14 - hypothetical protein (NCBI), at 13,508 to 14,152
_14
p5482_15: p5482_15 - TraM-like protein (NCBI), at 14,157 to 15,047
_15
p5482_16: p5482_16 - TraN-like protein (NCBI), at 15,044 to 16,141
_16
p5482_17: p5482_17 - hypothetical protein (NCBI), at 16,678 to 16,887
_17
p5482_18: p5482_18 - hypothetical protein (NCBI), at 16,898 to 17,605
_18
Position (kb) 15
16
17 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 14.108 kb on - strand at 14.110 kb on - strand at 14.110 kb on - strand at 14.110 kb on - strand at 14.128 kb on - strand at 14.151 kb on - strand at 14.157 kb on - strand at 14.161 kb on - strand at 14.295 kb on - strand, within p5482_15 at 14.295 kb on - strand, within p5482_15 at 14.331 kb on - strand, within p5482_15 at 14.382 kb on + strand, within p5482_15 at 14.403 kb on - strand, within p5482_15 at 14.406 kb on + strand, within p5482_15 at 14.407 kb on - strand, within p5482_15 at 14.429 kb on - strand, within p5482_15 at 14.446 kb on + strand, within p5482_15 at 14.447 kb on - strand, within p5482_15 at 14.447 kb on - strand, within p5482_15 at 14.560 kb on + strand, within p5482_15 at 14.561 kb on - strand, within p5482_15 at 14.657 kb on - strand, within p5482_15 at 14.705 kb on + strand, within p5482_15 at 14.706 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.761 kb on + strand, within p5482_15 at 14.796 kb on + strand, within p5482_15 at 14.797 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.844 kb on + strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.916 kb on - strand, within p5482_15 at 14.923 kb on - strand, within p5482_15 at 14.939 kb on - strand, within p5482_15 at 14.943 kb on - strand, within p5482_15 at 14.952 kb on - strand, within p5482_15 at 14.952 kb on - strand, within p5482_15 at 15.028 kb on - strand at 15.029 kb on + strand at 15.030 kb on - strand at 15.034 kb on + strand at 15.034 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.053 kb on - strand at 15.059 kb on - strand at 15.124 kb on + strand at 15.150 kb on + strand at 15.150 kb on + strand at 15.150 kb on + strand at 15.151 kb on - strand at 15.159 kb on + strand, within p5482_16 at 15.159 kb on + strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.181 kb on + strand, within p5482_16 at 15.181 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.233 kb on - strand, within p5482_16 at 15.233 kb on - strand, within p5482_16 at 15.323 kb on - strand, within p5482_16 at 15.332 kb on + strand, within p5482_16 at 15.332 kb on + strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.353 kb on - strand, within p5482_16 at 15.353 kb on - strand, within p5482_16 at 15.373 kb on + strand, within p5482_16 at 15.373 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.397 kb on + strand, within p5482_16 at 15.397 kb on + strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.401 kb on + strand, within p5482_16 at 15.452 kb on + strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.455 kb on - strand, within p5482_16 at 15.457 kb on - strand, within p5482_16 at 15.477 kb on + strand, within p5482_16 at 15.477 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.509 kb on - strand, within p5482_16 at 15.509 kb on - strand, within p5482_16 at 15.597 kb on - strand, within p5482_16 at 15.597 kb on - strand, within p5482_16 at 15.649 kb on - strand, within p5482_16 at 15.738 kb on + strand, within p5482_16 at 15.739 kb on - strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.743 kb on - strand at 15.815 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.873 kb on - strand, within p5482_16 at 15.873 kb on - strand, within p5482_16 at 15.878 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.884 kb on + strand, within p5482_16 at 15.885 kb on - strand, within p5482_16 at 15.887 kb on - strand, within p5482_16 at 15.897 kb on - strand, within p5482_16 at 16.025 kb on - strand, within p5482_16 at 16.139 kb on + strand at 16.140 kb on - strand at 16.140 kb on - strand at 16.140 kb on - strand at 16.234 kb on - strand at 16.281 kb on - strand at 16.324 kb on - strand at 16.424 kb on - strand at 16.487 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.543 kb on - strand at 16.610 kb on + strand at 16.630 kb on - strand at 16.630 kb on - strand at 16.640 kb on - strand at 16.657 kb on + strand at 16.666 kb on + strand at 16.667 kb on - strand at 16.686 kb on - strand at 16.686 kb on - strand at 16.686 kb on - strand at 16.689 kb on + strand at 16.689 kb on + strand at 16.703 kb on + strand, within p5482_17 at 16.703 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.817 kb on - strand, within p5482_17 at 16.869 kb on + strand at 16.870 kb on - strand at 16.886 kb on - strand at 16.890 kb on + strand at 16.898 kb on - strand at 16.909 kb on - strand at 16.999 kb on + strand, within p5482_18 at 16.999 kb on + strand, within p5482_18 at 17.000 kb on - strand, within p5482_18 at 17.001 kb on + strand, within p5482_18 at 17.019 kb on + strand, within p5482_18 at 17.047 kb on + strand, within p5482_18 at 17.047 kb on + strand, within p5482_18 at 17.048 kb on - strand, within p5482_18 at 17.085 kb on + strand, within p5482_18 at 17.085 kb on + strand, within p5482_18 at 17.086 kb on - strand, within p5482_18 at 17.086 kb on - strand, within p5482_18
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.1 mM remove 14,108 - -3.1 14,110 - -1.6 14,110 - -2.2 14,110 - +1.6 14,128 - +2.7 14,151 - -1.2 14,157 - +1.3 14,161 - -1.5 14,295 - p5482_15 0.15 -1.4 14,295 - p5482_15 0.15 -0.5 14,331 - p5482_15 0.20 +2.9 14,382 + p5482_15 0.25 -3.1 14,403 - p5482_15 0.28 +2.1 14,406 + p5482_15 0.28 -3.1 14,407 - p5482_15 0.28 +1.3 14,429 - p5482_15 0.31 +1.9 14,446 + p5482_15 0.32 -1.8 14,447 - p5482_15 0.33 -1.5 14,447 - p5482_15 0.33 -2.4 14,560 + p5482_15 0.45 -2.5 14,561 - p5482_15 0.45 -2.0 14,657 - p5482_15 0.56 -1.7 14,705 + p5482_15 0.62 +2.3 14,706 - p5482_15 0.62 -1.3 14,760 - p5482_15 0.68 -2.8 14,760 - p5482_15 0.68 +1.9 14,760 - p5482_15 0.68 -1.0 14,760 - p5482_15 0.68 -2.7 14,760 - p5482_15 0.68 -1.9 14,761 + p5482_15 0.68 -2.5 14,796 + p5482_15 0.72 -2.2 14,797 - p5482_15 0.72 +2.5 14,841 - p5482_15 0.77 -1.6 14,841 - p5482_15 0.77 +0.9 14,841 - p5482_15 0.77 -1.7 14,841 - p5482_15 0.77 -2.7 14,843 - p5482_15 0.77 -2.5 14,843 - p5482_15 0.77 -0.2 14,843 - p5482_15 0.77 -1.2 14,844 + p5482_15 0.77 -0.5 14,845 - p5482_15 0.77 -1.4 14,845 - p5482_15 0.77 -2.1 14,845 - p5482_15 0.77 -1.0 14,845 - p5482_15 0.77 -1.9 14,845 - p5482_15 0.77 -3.4 14,845 - p5482_15 0.77 -2.1 14,845 - p5482_15 0.77 -5.1 14,845 - p5482_15 0.77 +1.8 14,916 - p5482_15 0.85 +2.2 14,923 - p5482_15 0.86 -5.1 14,939 - p5482_15 0.88 -4.6 14,943 - p5482_15 0.88 +1.6 14,952 - p5482_15 0.89 +3.1 14,952 - p5482_15 0.89 -2.1 15,028 - -2.5 15,029 + -3.0 15,030 - -4.0 15,034 + -2.5 15,034 + -0.5 15,052 + -1.2 15,052 + +2.6 15,052 + +1.0 15,052 + -2.2 15,053 - -0.7 15,059 - -3.0 15,124 + -1.3 15,150 + -4.2 15,150 + +4.0 15,150 + -1.9 15,151 - +1.5 15,159 + p5482_16 0.10 -3.5 15,159 + p5482_16 0.10 -3.2 15,160 - p5482_16 0.11 -1.5 15,160 - p5482_16 0.11 -1.7 15,160 - p5482_16 0.11 -2.2 15,179 + p5482_16 0.12 -3.4 15,179 + p5482_16 0.12 -4.4 15,179 + p5482_16 0.12 -2.0 15,181 + p5482_16 0.12 -1.0 15,181 + p5482_16 0.12 -2.1 15,232 + p5482_16 0.17 -3.2 15,232 + p5482_16 0.17 -1.7 15,232 + p5482_16 0.17 -2.7 15,233 - p5482_16 0.17 -2.1 15,233 - p5482_16 0.17 +2.0 15,323 - p5482_16 0.25 -2.7 15,332 + p5482_16 0.26 -0.7 15,332 + p5482_16 0.26 -3.7 15,333 - p5482_16 0.26 -1.9 15,333 - p5482_16 0.26 +1.1 15,333 - p5482_16 0.26 -1.9 15,333 - p5482_16 0.26 +1.2 15,352 + p5482_16 0.28 -1.2 15,352 + p5482_16 0.28 -1.2 15,352 + p5482_16 0.28 -2.0 15,352 + p5482_16 0.28 -4.5 15,353 - p5482_16 0.28 +1.1 15,353 - p5482_16 0.28 -2.3 15,373 + p5482_16 0.30 -1.1 15,373 + p5482_16 0.30 -5.5 15,384 + p5482_16 0.31 -2.8 15,384 + p5482_16 0.31 -1.7 15,384 + p5482_16 0.31 -3.2 15,385 - p5482_16 0.31 +1.7 15,385 - p5482_16 0.31 -0.7 15,385 - p5482_16 0.31 -1.0 15,397 + p5482_16 0.32 -4.7 15,397 + p5482_16 0.32 -1.7 15,398 - p5482_16 0.32 -1.2 15,398 - p5482_16 0.32 -2.0 15,398 - p5482_16 0.32 -1.7 15,398 - p5482_16 0.32 +0.2 15,398 - p5482_16 0.32 +2.0 15,401 + p5482_16 0.33 -5.5 15,452 + p5482_16 0.37 -2.5 15,453 - p5482_16 0.37 -1.7 15,453 - p5482_16 0.37 -3.4 15,453 - p5482_16 0.37 -0.6 15,453 - p5482_16 0.37 -0.3 15,453 - p5482_16 0.37 -3.5 15,455 - p5482_16 0.37 -0.5 15,457 - p5482_16 0.38 -4.2 15,477 + p5482_16 0.39 -2.0 15,477 + p5482_16 0.39 -3.4 15,483 + p5482_16 0.40 -3.2 15,483 + p5482_16 0.40 -1.2 15,483 + p5482_16 0.40 -3.8 15,484 - p5482_16 0.40 -4.2 15,484 - p5482_16 0.40 +2.3 15,484 - p5482_16 0.40 +1.3 15,484 - p5482_16 0.40 -1.2 15,484 - p5482_16 0.40 +0.8 15,509 - p5482_16 0.42 -2.5 15,509 - p5482_16 0.42 -1.8 15,597 - p5482_16 0.50 -1.9 15,597 - p5482_16 0.50 -4.2 15,649 - p5482_16 0.55 +0.8 15,738 + p5482_16 0.63 -4.0 15,739 - p5482_16 0.63 -2.2 15,740 + p5482_16 0.63 +0.2 15,740 + p5482_16 0.63 -2.2 15,740 + p5482_16 0.63 +1.6 15,741 - p5482_16 0.63 +3.5 15,741 - p5482_16 0.63 +0.6 15,741 - p5482_16 0.63 -1.2 15,741 - p5482_16 0.63 +0.3 15,741 - p5482_16 0.63 -1.2 15,741 - p5482_16 0.63 -0.0 15,743 - +0.3 15,815 - p5482_16 0.70 +0.0 15,817 - p5482_16 0.70 +1.1 15,817 - p5482_16 0.70 -3.2 15,817 - p5482_16 0.70 +0.6 15,817 - p5482_16 0.70 +2.3 15,817 - p5482_16 0.70 +1.1 15,817 - p5482_16 0.70 +2.6 15,819 - p5482_16 0.71 -1.8 15,819 - p5482_16 0.71 -4.0 15,819 - p5482_16 0.71 -2.5 15,873 - p5482_16 0.76 -2.0 15,873 - p5482_16 0.76 +1.0 15,878 - p5482_16 0.76 +1.7 15,880 - p5482_16 0.76 -3.2 15,880 - p5482_16 0.76 +1.3 15,880 - p5482_16 0.76 -1.5 15,880 - p5482_16 0.76 -1.4 15,880 - p5482_16 0.76 -2.6 15,880 - p5482_16 0.76 +0.5 15,884 + p5482_16 0.77 +1.9 15,885 - p5482_16 0.77 +2.7 15,887 - p5482_16 0.77 -1.8 15,897 - p5482_16 0.78 +0.3 16,025 - p5482_16 0.89 -2.4 16,139 + +0.3 16,140 - -3.1 16,140 - +1.1 16,140 - -1.7 16,234 - -1.6 16,281 - -1.5 16,324 - -2.6 16,424 - -3.5 16,487 - -3.5 16,522 - -0.3 16,522 - +2.2 16,522 - +1.9 16,522 - +0.3 16,522 - +2.2 16,543 - -2.5 16,610 + -1.7 16,630 - +2.1 16,630 - +2.1 16,640 - -2.5 16,657 + -2.2 16,666 + +1.8 16,667 - -0.8 16,686 - -0.7 16,686 - -2.1 16,686 - -1.8 16,689 + +2.9 16,689 + +1.5 16,703 + p5482_17 0.12 -1.4 16,703 + p5482_17 0.12 -4.0 16,816 + p5482_17 0.66 -3.2 16,816 + p5482_17 0.66 +1.6 16,816 + p5482_17 0.66 -2.9 16,816 + p5482_17 0.66 -2.2 16,816 + p5482_17 0.66 +0.3 16,816 + p5482_17 0.66 +1.6 16,816 + p5482_17 0.66 -2.0 16,817 - p5482_17 0.66 -1.5 16,869 + -3.0 16,870 - -3.4 16,886 - +2.9 16,890 + -2.5 16,898 - -1.6 16,909 - +1.3 16,999 + p5482_18 0.14 -1.7 16,999 + p5482_18 0.14 -2.9 17,000 - p5482_18 0.14 -5.0 17,001 + p5482_18 0.15 -2.0 17,019 + p5482_18 0.17 -1.5 17,047 + p5482_18 0.21 +2.4 17,047 + p5482_18 0.21 -2.5 17,048 - p5482_18 0.21 -2.2 17,085 + p5482_18 0.26 +2.2 17,085 + p5482_18 0.26 -1.1 17,086 - p5482_18 0.27 -2.1 17,086 - p5482_18 0.27 -0.5
Or see this region's nucleotide sequence