Experiment: Varel_Bryant_medium_Glucose with Furaltadone 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1314 and BT1315 are separated by 494 nucleotides BT1315 and BT1316 are separated by 236 nucleotides BT1316 and BT1317 are separated by 57 nucleotides
BT1314: BT1314 - dipeptidyl-peptidase VI (NCBI ptt file), at 1,638,918 to 1,639,904
BT1314
BT1315: BT1315 - ribonuclease BN (NCBI ptt file), at 1,640,399 to 1,641,718
BT1315
BT1316: BT1316 - putative NADH dehydrogenase/NAD(P)H nitroreductase (NCBI ptt file), at 1,641,955 to 1,642,491
BT1316
BT1317: BT1317 - riboflavin synthase alpha chain (NCBI ptt file), at 1,642,549 to 1,643,151
BT1317
Position (kb)
1640
1641
1642 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1639.424 kb on + strand, within BT1314 at 1639.438 kb on + strand, within BT1314 at 1639.571 kb on - strand, within BT1314 at 1639.637 kb on + strand, within BT1314 at 1639.711 kb on - strand, within BT1314 at 1639.757 kb on + strand, within BT1314 at 1639.798 kb on - strand, within BT1314 at 1639.864 kb on - strand at 1639.917 kb on + strand at 1639.918 kb on - strand at 1640.473 kb on + strand at 1640.503 kb on + strand at 1640.555 kb on - strand, within BT1315 at 1640.574 kb on - strand, within BT1315 at 1640.576 kb on - strand, within BT1315 at 1640.595 kb on + strand, within BT1315 at 1640.644 kb on + strand, within BT1315 at 1640.807 kb on - strand, within BT1315 at 1640.880 kb on - strand, within BT1315 at 1640.943 kb on + strand, within BT1315 at 1640.943 kb on + strand, within BT1315 at 1640.947 kb on + strand, within BT1315 at 1640.948 kb on - strand, within BT1315 at 1641.037 kb on - strand, within BT1315 at 1641.146 kb on + strand, within BT1315 at 1641.177 kb on - strand, within BT1315 at 1641.188 kb on + strand, within BT1315 at 1641.189 kb on - strand, within BT1315 at 1641.406 kb on + strand, within BT1315 at 1641.468 kb on - strand, within BT1315 at 1641.527 kb on - strand, within BT1315 at 1641.655 kb on - strand at 1641.680 kb on + strand at 1641.680 kb on + strand at 1641.787 kb on + strand at 1641.803 kb on - strand at 1641.914 kb on - strand at 1641.916 kb on - strand at 1641.919 kb on + strand at 1641.940 kb on - strand at 1642.084 kb on + strand, within BT1316 at 1642.145 kb on - strand, within BT1316 at 1642.145 kb on - strand, within BT1316 at 1642.150 kb on - strand, within BT1316 at 1642.150 kb on - strand, within BT1316 at 1642.174 kb on - strand, within BT1316 at 1642.224 kb on + strand, within BT1316 at 1642.282 kb on - strand, within BT1316 at 1642.441 kb on - strand at 1642.555 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Furaltadone 0.1 mM remove 1,639,424 + BT1314 0.51 -0.0 1,639,438 + BT1314 0.53 +0.6 1,639,571 - BT1314 0.66 +1.6 1,639,637 + BT1314 0.73 -2.1 1,639,711 - BT1314 0.80 -1.3 1,639,757 + BT1314 0.85 -2.3 1,639,798 - BT1314 0.89 -2.0 1,639,864 - -0.0 1,639,917 + +0.4 1,639,918 - +0.3 1,640,473 + -6.6 1,640,503 + -1.5 1,640,555 - BT1315 0.12 +0.6 1,640,574 - BT1315 0.13 -2.3 1,640,576 - BT1315 0.13 -0.0 1,640,595 + BT1315 0.15 -1.6 1,640,644 + BT1315 0.19 -1.0 1,640,807 - BT1315 0.31 -1.0 1,640,880 - BT1315 0.36 -4.1 1,640,943 + BT1315 0.41 -3.3 1,640,943 + BT1315 0.41 -2.3 1,640,947 + BT1315 0.42 -0.0 1,640,948 - BT1315 0.42 -0.0 1,641,037 - BT1315 0.48 -1.0 1,641,146 + BT1315 0.57 -3.0 1,641,177 - BT1315 0.59 -2.0 1,641,188 + BT1315 0.60 -2.3 1,641,189 - BT1315 0.60 -1.3 1,641,406 + BT1315 0.76 -2.0 1,641,468 - BT1315 0.81 -2.0 1,641,527 - BT1315 0.85 -0.0 1,641,655 - -0.6 1,641,680 + -3.5 1,641,680 + -1.3 1,641,787 + -0.0 1,641,803 - -0.6 1,641,914 - +0.6 1,641,916 - -0.1 1,641,919 + -1.3 1,641,940 - -0.6 1,642,084 + BT1316 0.24 -2.3 1,642,145 - BT1316 0.35 -1.6 1,642,145 - BT1316 0.35 -0.5 1,642,150 - BT1316 0.36 -0.4 1,642,150 - BT1316 0.36 -3.0 1,642,174 - BT1316 0.41 -0.0 1,642,224 + BT1316 0.50 -2.3 1,642,282 - BT1316 0.61 -0.7 1,642,441 - -0.0 1,642,555 - -0.0
Or see this region's nucleotide sequence