Experiment: Varel_Bryant_medium_Glucose with Furaltadone 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1139 and BT1140 are separated by 150 nucleotides BT1140 and BT1141 are separated by 309 nucleotides BT1141 and BT1142 are separated by 9 nucleotides BT1142 and BT1143 are separated by 214 nucleotides
BT1139: BT1139 - conserved hypothetical protein (NCBI ptt file), at 1,423,594 to 1,424,652
BT1139
BT1140: BT1140 - hypothetical protein (NCBI ptt file), at 1,424,803 to 1,425,201
BT1140
BT1141: BT1141 - cation efflux family protein (NCBI ptt file), at 1,425,511 to 1,426,434
BT1141
BT1142: BT1142 - hypothetical protein (NCBI ptt file), at 1,426,444 to 1,426,929
BT1142
BT1143: BT1143 - conserved hypothetical protein (NCBI ptt file), at 1,427,144 to 1,427,344
BT1143
Position (kb)
1425
1426
1427 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1424.527 kb on - strand, within BT1139 at 1424.558 kb on - strand at 1424.657 kb on - strand at 1424.911 kb on + strand, within BT1140 at 1424.996 kb on + strand, within BT1140 at 1424.997 kb on - strand, within BT1140 at 1425.049 kb on - strand, within BT1140 at 1425.060 kb on + strand, within BT1140 at 1425.129 kb on + strand, within BT1140 at 1425.129 kb on + strand, within BT1140 at 1425.133 kb on + strand, within BT1140 at 1425.153 kb on + strand, within BT1140 at 1425.222 kb on + strand at 1425.244 kb on + strand at 1425.300 kb on + strand at 1425.303 kb on + strand at 1425.362 kb on + strand at 1425.415 kb on + strand at 1425.448 kb on - strand at 1425.478 kb on - strand at 1425.478 kb on - strand at 1425.479 kb on + strand at 1425.480 kb on - strand at 1425.482 kb on - strand at 1425.534 kb on - strand at 1425.562 kb on - strand at 1425.600 kb on - strand at 1425.725 kb on + strand, within BT1141 at 1425.780 kb on - strand, within BT1141 at 1425.813 kb on + strand, within BT1141 at 1425.823 kb on - strand, within BT1141 at 1425.868 kb on + strand, within BT1141 at 1425.869 kb on - strand, within BT1141 at 1425.911 kb on + strand, within BT1141 at 1425.912 kb on - strand, within BT1141 at 1425.912 kb on - strand, within BT1141 at 1425.942 kb on + strand, within BT1141 at 1425.943 kb on - strand, within BT1141 at 1425.943 kb on - strand, within BT1141 at 1425.987 kb on + strand at 1425.988 kb on - strand, within BT1141 at 1426.047 kb on - strand, within BT1141 at 1426.067 kb on + strand, within BT1141 at 1426.084 kb on - strand, within BT1141 at 1426.164 kb on + strand, within BT1141 at 1426.341 kb on - strand, within BT1141 at 1426.351 kb on + strand at 1426.357 kb on - strand at 1426.362 kb on - strand at 1426.364 kb on + strand at 1426.457 kb on - strand at 1426.457 kb on - strand at 1426.458 kb on + strand at 1426.683 kb on + strand, within BT1142 at 1426.684 kb on - strand, within BT1142 at 1426.686 kb on - strand, within BT1142 at 1426.747 kb on - strand, within BT1142 at 1426.762 kb on - strand, within BT1142 at 1426.825 kb on + strand, within BT1142 at 1426.860 kb on + strand, within BT1142 at 1426.860 kb on + strand, within BT1142 at 1426.910 kb on - strand at 1426.929 kb on + strand at 1426.990 kb on + strand at 1427.070 kb on + strand at 1427.121 kb on - strand at 1427.121 kb on - strand at 1427.157 kb on - strand at 1427.176 kb on + strand, within BT1143 at 1427.246 kb on - strand, within BT1143 at 1427.249 kb on - strand, within BT1143 at 1427.323 kb on + strand, within BT1143 at 1427.367 kb on - strand at 1427.426 kb on + strand at 1427.431 kb on + strand at 1427.432 kb on - strand at 1427.432 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Furaltadone 0.1 mM remove 1,424,527 - BT1139 0.88 -1.1 1,424,558 - -1.7 1,424,657 - -0.8 1,424,911 + BT1140 0.27 -1.6 1,424,996 + BT1140 0.48 +1.1 1,424,997 - BT1140 0.49 -1.3 1,425,049 - BT1140 0.62 -0.1 1,425,060 + BT1140 0.64 -0.8 1,425,129 + BT1140 0.82 -2.1 1,425,129 + BT1140 0.82 +1.9 1,425,133 + BT1140 0.83 -0.1 1,425,153 + BT1140 0.88 +2.2 1,425,222 + -1.1 1,425,244 + +0.3 1,425,300 + -2.1 1,425,303 + -0.1 1,425,362 + +1.1 1,425,415 + +0.9 1,425,448 - -0.1 1,425,478 - -1.7 1,425,478 - -0.4 1,425,479 + +0.6 1,425,480 - -2.3 1,425,482 - -1.1 1,425,534 - -0.1 1,425,562 - -0.1 1,425,600 - -2.4 1,425,725 + BT1141 0.23 -2.0 1,425,780 - BT1141 0.29 -1.1 1,425,813 + BT1141 0.33 -1.7 1,425,823 - BT1141 0.34 -2.1 1,425,868 + BT1141 0.39 -2.8 1,425,869 - BT1141 0.39 -2.1 1,425,911 + BT1141 0.43 -1.1 1,425,912 - BT1141 0.43 +0.9 1,425,912 - BT1141 0.43 -0.1 1,425,942 + BT1141 0.47 -1.4 1,425,943 - BT1141 0.47 -0.1 1,425,943 - BT1141 0.47 -2.5 1,425,987 + -0.1 1,425,988 - BT1141 0.52 -3.1 1,426,047 - BT1141 0.58 -0.1 1,426,067 + BT1141 0.60 -1.1 1,426,084 - BT1141 0.62 -1.0 1,426,164 + BT1141 0.71 -1.1 1,426,341 - BT1141 0.90 -1.7 1,426,351 + -1.7 1,426,357 - +0.5 1,426,362 - -0.1 1,426,364 + -1.1 1,426,457 - -2.4 1,426,457 - -0.1 1,426,458 + -0.8 1,426,683 + BT1142 0.49 -0.1 1,426,684 - BT1142 0.49 -0.1 1,426,686 - BT1142 0.50 -0.6 1,426,747 - BT1142 0.62 +2.2 1,426,762 - BT1142 0.65 -1.1 1,426,825 + BT1142 0.78 -1.2 1,426,860 + BT1142 0.86 -1.1 1,426,860 + BT1142 0.86 -1.7 1,426,910 - -0.1 1,426,929 + -2.3 1,426,990 + -1.1 1,427,070 + -1.1 1,427,121 - -0.1 1,427,121 - -0.1 1,427,157 - -0.1 1,427,176 + BT1143 0.16 -1.5 1,427,246 - BT1143 0.51 -2.8 1,427,249 - BT1143 0.52 -0.1 1,427,323 + BT1143 0.89 +0.9 1,427,367 - +2.5 1,427,426 + -0.1 1,427,431 + -0.1 1,427,432 - -0.1 1,427,432 - +0.3
Or see this region's nucleotide sequence