Experiment: Shikimic Acid 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_006122 and ABIE53_006123 are separated by 372 nucleotides ABIE53_006123 and ABIE53_006124 are separated by 78 nucleotides ABIE53_006124 and ABIE53_006125 are separated by 267 nucleotides
ABIE53_006122: ABIE53_006122 - nucleotide-binding universal stress UspA family protein, at 2,461,922 to 2,462,395
_006122
ABIE53_006123: ABIE53_006123 - hypothetical protein, at 2,462,768 to 2,463,064
_006123
ABIE53_006124: ABIE53_006124 - outer membrane protein OmpU, at 2,463,143 to 2,464,291
_006124
ABIE53_006125: ABIE53_006125 - glycine betaine/proline transport system substrate-binding protein, at 2,464,559 to 2,465,512
_006125
Position (kb)
2463
2464
2465 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2462.256 kb on + strand, within ABIE53_006122 at 2462.257 kb on - strand, within ABIE53_006122 at 2462.257 kb on - strand, within ABIE53_006122 at 2462.257 kb on - strand, within ABIE53_006122 at 2462.287 kb on - strand, within ABIE53_006122 at 2462.287 kb on - strand, within ABIE53_006122 at 2462.391 kb on + strand at 2462.391 kb on + strand at 2462.392 kb on - strand at 2462.392 kb on - strand at 2462.392 kb on - strand at 2462.643 kb on - strand at 2462.894 kb on + strand, within ABIE53_006123 at 2462.894 kb on + strand, within ABIE53_006123 at 2462.934 kb on - strand, within ABIE53_006123 at 2462.934 kb on - strand, within ABIE53_006123 at 2462.934 kb on - strand, within ABIE53_006123 at 2462.982 kb on - strand, within ABIE53_006123 at 2463.041 kb on - strand at 2463.264 kb on + strand, within ABIE53_006124 at 2463.264 kb on + strand, within ABIE53_006124 at 2463.264 kb on + strand, within ABIE53_006124 at 2463.274 kb on + strand, within ABIE53_006124 at 2463.275 kb on - strand, within ABIE53_006124 at 2463.357 kb on + strand, within ABIE53_006124 at 2463.447 kb on + strand, within ABIE53_006124 at 2463.447 kb on + strand, within ABIE53_006124 at 2463.485 kb on - strand, within ABIE53_006124 at 2463.487 kb on - strand, within ABIE53_006124 at 2463.604 kb on + strand, within ABIE53_006124 at 2463.604 kb on + strand, within ABIE53_006124 at 2463.605 kb on - strand, within ABIE53_006124 at 2463.605 kb on - strand, within ABIE53_006124 at 2463.625 kb on - strand, within ABIE53_006124 at 2463.640 kb on - strand, within ABIE53_006124 at 2463.640 kb on - strand, within ABIE53_006124 at 2463.703 kb on - strand, within ABIE53_006124 at 2463.793 kb on + strand, within ABIE53_006124 at 2463.897 kb on + strand, within ABIE53_006124 at 2463.898 kb on - strand, within ABIE53_006124 at 2463.975 kb on + strand, within ABIE53_006124 at 2463.975 kb on + strand, within ABIE53_006124 at 2464.025 kb on + strand, within ABIE53_006124 at 2464.026 kb on - strand, within ABIE53_006124 at 2464.026 kb on - strand, within ABIE53_006124 at 2464.046 kb on + strand, within ABIE53_006124 at 2464.164 kb on + strand, within ABIE53_006124 at 2464.165 kb on - strand, within ABIE53_006124 at 2464.165 kb on - strand, within ABIE53_006124 at 2464.310 kb on - strand at 2464.429 kb on - strand at 2464.478 kb on + strand at 2464.561 kb on - strand at 2464.706 kb on + strand, within ABIE53_006125 at 2464.748 kb on - strand, within ABIE53_006125 at 2464.748 kb on - strand, within ABIE53_006125 at 2464.845 kb on + strand, within ABIE53_006125 at 2464.845 kb on + strand, within ABIE53_006125 at 2464.845 kb on + strand, within ABIE53_006125 at 2464.846 kb on - strand, within ABIE53_006125 at 2465.070 kb on + strand, within ABIE53_006125 at 2465.070 kb on + strand, within ABIE53_006125 at 2465.071 kb on - strand, within ABIE53_006125 at 2465.071 kb on - strand, within ABIE53_006125 at 2465.072 kb on + strand, within ABIE53_006125 at 2465.264 kb on + strand, within ABIE53_006125
Per-strain Table
Position Strand Gene LocusTag Fraction Shikimic Acid 5 mM (C) remove 2,462,256 + ABIE53_006122 0.70 -0.0 2,462,257 - ABIE53_006122 0.71 -0.6 2,462,257 - ABIE53_006122 0.71 -0.0 2,462,257 - ABIE53_006122 0.71 +0.2 2,462,287 - ABIE53_006122 0.77 -1.8 2,462,287 - ABIE53_006122 0.77 -1.0 2,462,391 + -3.6 2,462,391 + +1.0 2,462,392 - -0.4 2,462,392 - +2.0 2,462,392 - -0.0 2,462,643 - +2.0 2,462,894 + ABIE53_006123 0.42 -0.6 2,462,894 + ABIE53_006123 0.42 -2.0 2,462,934 - ABIE53_006123 0.56 -2.0 2,462,934 - ABIE53_006123 0.56 -0.0 2,462,934 - ABIE53_006123 0.56 -0.6 2,462,982 - ABIE53_006123 0.72 -0.6 2,463,041 - -2.2 2,463,264 + ABIE53_006124 0.11 -0.0 2,463,264 + ABIE53_006124 0.11 +1.0 2,463,264 + ABIE53_006124 0.11 -3.0 2,463,274 + ABIE53_006124 0.11 +2.0 2,463,275 - ABIE53_006124 0.11 -0.4 2,463,357 + ABIE53_006124 0.19 +0.1 2,463,447 + ABIE53_006124 0.26 -0.8 2,463,447 + ABIE53_006124 0.26 -0.0 2,463,485 - ABIE53_006124 0.30 -0.2 2,463,487 - ABIE53_006124 0.30 -2.3 2,463,604 + ABIE53_006124 0.40 -2.7 2,463,604 + ABIE53_006124 0.40 -0.0 2,463,605 - ABIE53_006124 0.40 -0.0 2,463,605 - ABIE53_006124 0.40 +2.0 2,463,625 - ABIE53_006124 0.42 -2.2 2,463,640 - ABIE53_006124 0.43 +1.0 2,463,640 - ABIE53_006124 0.43 -0.6 2,463,703 - ABIE53_006124 0.49 -1.3 2,463,793 + ABIE53_006124 0.57 -0.0 2,463,897 + ABIE53_006124 0.66 +0.4 2,463,898 - ABIE53_006124 0.66 -2.9 2,463,975 + ABIE53_006124 0.72 -0.0 2,463,975 + ABIE53_006124 0.72 -1.8 2,464,025 + ABIE53_006124 0.77 -0.0 2,464,026 - ABIE53_006124 0.77 -1.4 2,464,026 - ABIE53_006124 0.77 -0.6 2,464,046 + ABIE53_006124 0.79 -0.0 2,464,164 + ABIE53_006124 0.89 -2.8 2,464,165 - ABIE53_006124 0.89 -3.2 2,464,165 - ABIE53_006124 0.89 -1.3 2,464,310 - -0.0 2,464,429 - +0.9 2,464,478 + -1.3 2,464,561 - +1.0 2,464,706 + ABIE53_006125 0.15 +2.6 2,464,748 - ABIE53_006125 0.20 +1.0 2,464,748 - ABIE53_006125 0.20 +1.0 2,464,845 + ABIE53_006125 0.30 -0.0 2,464,845 + ABIE53_006125 0.30 -1.0 2,464,845 + ABIE53_006125 0.30 -2.7 2,464,846 - ABIE53_006125 0.30 +1.0 2,465,070 + ABIE53_006125 0.54 -0.0 2,465,070 + ABIE53_006125 0.54 -1.3 2,465,071 - ABIE53_006125 0.54 +2.6 2,465,071 - ABIE53_006125 0.54 -0.2 2,465,072 + ABIE53_006125 0.54 +2.0 2,465,264 + ABIE53_006125 0.74 -1.3
Or see this region's nucleotide sequence