Experiment: Shikimic Acid 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_005227 and ABIE53_005228 are separated by 29 nucleotides ABIE53_005228 and ABIE53_005229 overlap by 4 nucleotides ABIE53_005229 and ABIE53_005230 are separated by 192 nucleotides
ABIE53_005227: ABIE53_005227 - 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase, at 1,484,588 to 1,486,045
_005227
ABIE53_005228: ABIE53_005228 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase/2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 1,486,075 to 1,486,833
_005228
ABIE53_005229: ABIE53_005229 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase/2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 1,486,830 to 1,487,615
_005229
ABIE53_005230: ABIE53_005230 - homoprotocatechuate degradation regulator HpaR, at 1,487,808 to 1,488,215
_005230
Position (kb)
1486
1487 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1485.217 kb on + strand, within ABIE53_005227 at 1485.658 kb on - strand, within ABIE53_005227 at 1485.663 kb on - strand, within ABIE53_005227 at 1485.663 kb on - strand, within ABIE53_005227 at 1485.682 kb on - strand, within ABIE53_005227 at 1485.685 kb on + strand, within ABIE53_005227 at 1485.720 kb on + strand, within ABIE53_005227 at 1486.057 kb on + strand at 1486.376 kb on - strand, within ABIE53_005228 at 1486.421 kb on + strand, within ABIE53_005228 at 1486.422 kb on - strand, within ABIE53_005228 at 1486.422 kb on - strand, within ABIE53_005228 at 1486.501 kb on + strand, within ABIE53_005228 at 1486.502 kb on - strand, within ABIE53_005228 at 1486.502 kb on - strand, within ABIE53_005228 at 1486.502 kb on - strand, within ABIE53_005228 at 1486.547 kb on + strand, within ABIE53_005228 at 1486.548 kb on - strand, within ABIE53_005228 at 1486.749 kb on + strand, within ABIE53_005228 at 1486.750 kb on - strand, within ABIE53_005228 at 1487.020 kb on + strand, within ABIE53_005229 at 1487.020 kb on + strand, within ABIE53_005229 at 1487.020 kb on + strand, within ABIE53_005229 at 1487.020 kb on + strand, within ABIE53_005229 at 1487.179 kb on + strand, within ABIE53_005229 at 1487.182 kb on - strand, within ABIE53_005229 at 1487.390 kb on - strand, within ABIE53_005229 at 1487.390 kb on - strand, within ABIE53_005229 at 1487.390 kb on - strand, within ABIE53_005229 at 1487.419 kb on + strand, within ABIE53_005229 at 1487.420 kb on - strand, within ABIE53_005229 at 1487.485 kb on + strand, within ABIE53_005229 at 1487.486 kb on - strand, within ABIE53_005229 at 1487.486 kb on - strand, within ABIE53_005229 at 1487.682 kb on + strand at 1487.762 kb on + strand at 1487.762 kb on + strand at 1487.762 kb on + strand at 1487.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Shikimic Acid 5 mM (C) remove 1,485,217 + ABIE53_005227 0.43 -0.2 1,485,658 - ABIE53_005227 0.73 +0.8 1,485,663 - ABIE53_005227 0.74 -0.6 1,485,663 - ABIE53_005227 0.74 -1.7 1,485,682 - ABIE53_005227 0.75 -0.8 1,485,685 + ABIE53_005227 0.75 -1.9 1,485,720 + ABIE53_005227 0.78 -0.2 1,486,057 + -2.8 1,486,376 - ABIE53_005228 0.40 -1.2 1,486,421 + ABIE53_005228 0.46 -2.4 1,486,422 - ABIE53_005228 0.46 +3.6 1,486,422 - ABIE53_005228 0.46 +1.8 1,486,501 + ABIE53_005228 0.56 -2.2 1,486,502 - ABIE53_005228 0.56 -0.2 1,486,502 - ABIE53_005228 0.56 +2.4 1,486,502 - ABIE53_005228 0.56 -1.8 1,486,547 + ABIE53_005228 0.62 +0.4 1,486,548 - ABIE53_005228 0.62 +0.8 1,486,749 + ABIE53_005228 0.89 +1.8 1,486,750 - ABIE53_005228 0.89 +0.8 1,487,020 + ABIE53_005229 0.24 -0.8 1,487,020 + ABIE53_005229 0.24 -2.1 1,487,020 + ABIE53_005229 0.24 -0.8 1,487,020 + ABIE53_005229 0.24 +0.8 1,487,179 + ABIE53_005229 0.44 +2.4 1,487,182 - ABIE53_005229 0.45 -1.5 1,487,390 - ABIE53_005229 0.71 -0.5 1,487,390 - ABIE53_005229 0.71 +0.4 1,487,390 - ABIE53_005229 0.71 +1.1 1,487,419 + ABIE53_005229 0.75 -1.1 1,487,420 - ABIE53_005229 0.75 -2.0 1,487,485 + ABIE53_005229 0.83 +0.2 1,487,486 - ABIE53_005229 0.83 +1.8 1,487,486 - ABIE53_005229 0.83 +0.8 1,487,682 + +1.8 1,487,762 + +0.6 1,487,762 + -0.5 1,487,762 + +2.8 1,487,763 - +0.8
Or see this region's nucleotide sequence