Experiment: KB with Totarol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_5100 and Psyr_5101 are separated by 159 nucleotides Psyr_5101 and Psyr_5102 are separated by 275 nucleotides Psyr_5102 and Psyr_5103 are separated by 1 nucleotides Psyr_5103 and Psyr_5104 overlap by 4 nucleotides
Psyr_5100: Psyr_5100 - conserved hypothetical protein, at 6,052,034 to 6,052,981
_5100
Psyr_5101: Psyr_5101 - Glyoxalase/bleomycin resistance protein/dioxygenase, at 6,053,141 to 6,053,554
_5101
Psyr_5102: Psyr_5102 - ABC transporter, at 6,053,830 to 6,054,609
_5102
Psyr_5103: Psyr_5103 - Periplasmic binding protein, at 6,054,611 to 6,055,561
_5103
Psyr_5104: Psyr_5104 - transport system permease protein, at 6,055,558 to 6,056,601
_5104
Position (kb)
6053
6054
6055 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 6052.923 kb on - strand at 6052.940 kb on - strand at 6052.992 kb on + strand at 6052.993 kb on - strand at 6053.001 kb on + strand at 6053.001 kb on + strand at 6053.001 kb on + strand at 6053.002 kb on - strand at 6053.012 kb on + strand at 6053.012 kb on + strand at 6053.012 kb on + strand at 6053.012 kb on + strand at 6053.012 kb on + strand at 6053.012 kb on + strand at 6053.012 kb on + strand at 6053.012 kb on + strand at 6053.012 kb on + strand at 6053.013 kb on - strand at 6053.013 kb on - strand at 6053.013 kb on - strand at 6053.013 kb on - strand at 6053.013 kb on - strand at 6053.013 kb on - strand at 6053.014 kb on + strand at 6053.023 kb on + strand at 6053.023 kb on + strand at 6053.024 kb on - strand at 6053.177 kb on + strand at 6053.177 kb on + strand at 6053.178 kb on - strand at 6053.178 kb on - strand at 6053.178 kb on - strand at 6053.181 kb on + strand at 6053.181 kb on + strand at 6053.181 kb on + strand at 6053.181 kb on + strand at 6053.182 kb on - strand at 6053.182 kb on - strand at 6053.182 kb on - strand at 6053.210 kb on + strand, within Psyr_5101 at 6053.210 kb on + strand, within Psyr_5101 at 6053.210 kb on + strand, within Psyr_5101 at 6053.210 kb on + strand, within Psyr_5101 at 6053.210 kb on + strand, within Psyr_5101 at 6053.211 kb on - strand, within Psyr_5101 at 6053.211 kb on - strand, within Psyr_5101 at 6053.211 kb on - strand, within Psyr_5101 at 6053.211 kb on - strand, within Psyr_5101 at 6053.328 kb on - strand, within Psyr_5101 at 6053.346 kb on + strand, within Psyr_5101 at 6053.346 kb on + strand, within Psyr_5101 at 6053.346 kb on + strand, within Psyr_5101 at 6053.346 kb on + strand, within Psyr_5101 at 6053.347 kb on - strand, within Psyr_5101 at 6053.347 kb on - strand, within Psyr_5101 at 6053.549 kb on + strand at 6053.550 kb on - strand at 6053.612 kb on + strand at 6053.612 kb on + strand at 6053.618 kb on + strand at 6053.669 kb on + strand at 6053.670 kb on - strand at 6053.670 kb on - strand at 6053.767 kb on + strand at 6053.767 kb on + strand at 6053.767 kb on + strand at 6053.767 kb on + strand at 6053.767 kb on + strand at 6053.768 kb on - strand at 6053.998 kb on + strand, within Psyr_5102 at 6054.203 kb on + strand, within Psyr_5102 at 6054.204 kb on - strand, within Psyr_5102 at 6054.204 kb on - strand, within Psyr_5102 at 6054.651 kb on + strand at 6054.651 kb on + strand at 6054.652 kb on - strand at 6054.652 kb on - strand at 6054.652 kb on - strand at 6054.652 kb on - strand at 6054.850 kb on - strand, within Psyr_5103 at 6054.850 kb on - strand, within Psyr_5103 at 6054.883 kb on + strand, within Psyr_5103 at 6054.884 kb on - strand, within Psyr_5103 at 6054.884 kb on - strand, within Psyr_5103 at 6054.887 kb on + strand, within Psyr_5103 at 6054.887 kb on + strand, within Psyr_5103 at 6054.887 kb on + strand, within Psyr_5103 at 6054.888 kb on - strand, within Psyr_5103 at 6054.929 kb on + strand, within Psyr_5103 at 6054.929 kb on + strand, within Psyr_5103 at 6054.930 kb on - strand, within Psyr_5103 at 6054.930 kb on - strand, within Psyr_5103 at 6054.930 kb on - strand, within Psyr_5103 at 6054.930 kb on - strand, within Psyr_5103 at 6054.947 kb on + strand, within Psyr_5103 at 6054.947 kb on + strand, within Psyr_5103 at 6054.948 kb on - strand, within Psyr_5103 at 6054.948 kb on - strand, within Psyr_5103 at 6054.948 kb on - strand, within Psyr_5103 at 6055.010 kb on + strand, within Psyr_5103 at 6055.010 kb on + strand, within Psyr_5103 at 6055.011 kb on - strand, within Psyr_5103 at 6055.039 kb on - strand, within Psyr_5103 at 6055.039 kb on - strand, within Psyr_5103 at 6055.039 kb on - strand, within Psyr_5103 at 6055.073 kb on + strand, within Psyr_5103 at 6055.074 kb on - strand, within Psyr_5103 at 6055.074 kb on - strand, within Psyr_5103 at 6055.306 kb on - strand, within Psyr_5103 at 6055.466 kb on + strand, within Psyr_5103 at 6055.466 kb on + strand, within Psyr_5103 at 6055.467 kb on - strand at 6055.481 kb on + strand at 6055.481 kb on + strand at 6055.482 kb on - strand at 6055.482 kb on - strand at 6055.482 kb on - strand at 6055.482 kb on - strand at 6055.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Totarol 10 mM remove 6,052,923 - +1.1 6,052,940 - -2.3 6,052,992 + +0.5 6,052,993 - +0.3 6,053,001 + -2.2 6,053,001 + -0.4 6,053,001 + +0.7 6,053,002 - -0.5 6,053,012 + -0.2 6,053,012 + +0.0 6,053,012 + +0.9 6,053,012 + -1.1 6,053,012 + +1.1 6,053,012 + -1.9 6,053,012 + -2.6 6,053,012 + -0.4 6,053,012 + +0.4 6,053,013 - +0.9 6,053,013 - +1.7 6,053,013 - -0.8 6,053,013 - +0.4 6,053,013 - -0.1 6,053,013 - -0.5 6,053,014 + +0.0 6,053,023 + +0.5 6,053,023 + -2.1 6,053,024 - +0.3 6,053,177 + -0.5 6,053,177 + -0.9 6,053,178 - -0.1 6,053,178 - +0.7 6,053,178 - -0.6 6,053,181 + +0.9 6,053,181 + +0.9 6,053,181 + +0.1 6,053,181 + +0.5 6,053,182 - -0.6 6,053,182 - -0.3 6,053,182 - -0.2 6,053,210 + Psyr_5101 0.17 -0.8 6,053,210 + Psyr_5101 0.17 +0.3 6,053,210 + Psyr_5101 0.17 +0.2 6,053,210 + Psyr_5101 0.17 +0.9 6,053,210 + Psyr_5101 0.17 -0.6 6,053,211 - Psyr_5101 0.17 -0.5 6,053,211 - Psyr_5101 0.17 +0.2 6,053,211 - Psyr_5101 0.17 +0.7 6,053,211 - Psyr_5101 0.17 +0.7 6,053,328 - Psyr_5101 0.45 -1.9 6,053,346 + Psyr_5101 0.50 +0.3 6,053,346 + Psyr_5101 0.50 -0.8 6,053,346 + Psyr_5101 0.50 -0.7 6,053,346 + Psyr_5101 0.50 +0.1 6,053,347 - Psyr_5101 0.50 -0.1 6,053,347 - Psyr_5101 0.50 -0.3 6,053,549 + -1.6 6,053,550 - +0.4 6,053,612 + +0.1 6,053,612 + +1.2 6,053,618 + -1.1 6,053,669 + +1.2 6,053,670 - -1.4 6,053,670 - +0.3 6,053,767 + -0.2 6,053,767 + +0.9 6,053,767 + +1.1 6,053,767 + +0.4 6,053,767 + -0.1 6,053,768 - +1.3 6,053,998 + Psyr_5102 0.22 -0.1 6,054,203 + Psyr_5102 0.48 -0.4 6,054,204 - Psyr_5102 0.48 -1.6 6,054,204 - Psyr_5102 0.48 -0.3 6,054,651 + +1.7 6,054,651 + -0.8 6,054,652 - -0.5 6,054,652 - +0.5 6,054,652 - -0.1 6,054,652 - +0.5 6,054,850 - Psyr_5103 0.25 -0.8 6,054,850 - Psyr_5103 0.25 -0.8 6,054,883 + Psyr_5103 0.29 +0.7 6,054,884 - Psyr_5103 0.29 -2.3 6,054,884 - Psyr_5103 0.29 -0.5 6,054,887 + Psyr_5103 0.29 -0.5 6,054,887 + Psyr_5103 0.29 +0.7 6,054,887 + Psyr_5103 0.29 -1.3 6,054,888 - Psyr_5103 0.29 +0.0 6,054,929 + Psyr_5103 0.33 +0.1 6,054,929 + Psyr_5103 0.33 +1.2 6,054,930 - Psyr_5103 0.34 -2.8 6,054,930 - Psyr_5103 0.34 +0.2 6,054,930 - Psyr_5103 0.34 -0.8 6,054,930 - Psyr_5103 0.34 +4.1 6,054,947 + Psyr_5103 0.35 +0.2 6,054,947 + Psyr_5103 0.35 -0.5 6,054,948 - Psyr_5103 0.35 +0.5 6,054,948 - Psyr_5103 0.35 +0.8 6,054,948 - Psyr_5103 0.35 +1.3 6,055,010 + Psyr_5103 0.42 -0.0 6,055,010 + Psyr_5103 0.42 -1.2 6,055,011 - Psyr_5103 0.42 +0.7 6,055,039 - Psyr_5103 0.45 -0.3 6,055,039 - Psyr_5103 0.45 -0.8 6,055,039 - Psyr_5103 0.45 -1.9 6,055,073 + Psyr_5103 0.49 -3.0 6,055,074 - Psyr_5103 0.49 +0.3 6,055,074 - Psyr_5103 0.49 +0.2 6,055,306 - Psyr_5103 0.73 -0.9 6,055,466 + Psyr_5103 0.90 +0.7 6,055,466 + Psyr_5103 0.90 +1.0 6,055,467 - -0.3 6,055,481 + +0.1 6,055,481 + +0.3 6,055,482 - -0.6 6,055,482 - -0.1 6,055,482 - +1.6 6,055,482 - -0.8 6,055,482 - -0.6
Or see this region's nucleotide sequence