Experiment: KB with Totarol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2148 and Psyr_2149 are separated by 4 nucleotides Psyr_2149 and Psyr_2150 are separated by 137 nucleotides Psyr_2150 and Psyr_2151 are separated by 193 nucleotides
Psyr_2148: Psyr_2148 - Deoxyribonuclease I, at 2,505,150 to 2,505,851
_2148
Psyr_2149: Psyr_2149 - conserved hypothetical protein, at 2,505,856 to 2,506,107
_2149
Psyr_2150: Psyr_2150 - protein of unknown function DUF903, at 2,506,245 to 2,506,469
_2150
Psyr_2151: Psyr_2151 - monosaccharide ABC transporter substrate-binding protein, CUT2 family, at 2,506,663 to 2,507,622
_2151
Position (kb)
2506
2507 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2505.274 kb on + strand, within Psyr_2148 at 2505.310 kb on + strand, within Psyr_2148 at 2505.310 kb on + strand, within Psyr_2148 at 2505.311 kb on - strand, within Psyr_2148 at 2505.311 kb on - strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.325 kb on + strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.326 kb on - strand, within Psyr_2148 at 2505.331 kb on + strand, within Psyr_2148 at 2505.331 kb on + strand, within Psyr_2148 at 2505.498 kb on - strand, within Psyr_2148 at 2505.498 kb on - strand, within Psyr_2148 at 2505.623 kb on + strand, within Psyr_2148 at 2505.659 kb on - strand, within Psyr_2148 at 2505.694 kb on + strand, within Psyr_2148 at 2505.694 kb on + strand, within Psyr_2148 at 2505.695 kb on - strand, within Psyr_2148 at 2505.695 kb on - strand, within Psyr_2148 at 2505.709 kb on + strand, within Psyr_2148 at 2505.733 kb on + strand, within Psyr_2148 at 2505.734 kb on - strand, within Psyr_2148 at 2505.734 kb on - strand, within Psyr_2148 at 2505.734 kb on - strand, within Psyr_2148 at 2505.734 kb on - strand, within Psyr_2148 at 2505.937 kb on + strand, within Psyr_2149 at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.175 kb on + strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.176 kb on - strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.179 kb on + strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.180 kb on - strand at 2506.185 kb on - strand at 2506.251 kb on - strand at 2506.347 kb on + strand, within Psyr_2150 at 2506.347 kb on + strand, within Psyr_2150 at 2506.361 kb on + strand, within Psyr_2150 at 2506.361 kb on + strand, within Psyr_2150 at 2506.362 kb on - strand, within Psyr_2150 at 2506.406 kb on + strand, within Psyr_2150 at 2506.528 kb on - strand at 2506.532 kb on - strand at 2506.639 kb on - strand at 2506.639 kb on - strand at 2506.744 kb on + strand at 2507.134 kb on + strand, within Psyr_2151 at 2507.434 kb on + strand, within Psyr_2151
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Totarol 10 mM remove 2,505,274 + Psyr_2148 0.18 -1.1 2,505,310 + Psyr_2148 0.23 +0.2 2,505,310 + Psyr_2148 0.23 -0.1 2,505,311 - Psyr_2148 0.23 +0.1 2,505,311 - Psyr_2148 0.23 +0.6 2,505,325 + Psyr_2148 0.25 -1.0 2,505,325 + Psyr_2148 0.25 +1.9 2,505,325 + Psyr_2148 0.25 +0.7 2,505,325 + Psyr_2148 0.25 +0.4 2,505,325 + Psyr_2148 0.25 +0.5 2,505,325 + Psyr_2148 0.25 +0.0 2,505,326 - Psyr_2148 0.25 +1.1 2,505,326 - Psyr_2148 0.25 +0.5 2,505,326 - Psyr_2148 0.25 +1.9 2,505,326 - Psyr_2148 0.25 +3.7 2,505,326 - Psyr_2148 0.25 +1.6 2,505,326 - Psyr_2148 0.25 +1.1 2,505,331 + Psyr_2148 0.26 +0.2 2,505,331 + Psyr_2148 0.26 -0.5 2,505,498 - Psyr_2148 0.50 +0.7 2,505,498 - Psyr_2148 0.50 +0.3 2,505,623 + Psyr_2148 0.67 -0.3 2,505,659 - Psyr_2148 0.73 -0.1 2,505,694 + Psyr_2148 0.77 +2.4 2,505,694 + Psyr_2148 0.77 +0.8 2,505,695 - Psyr_2148 0.78 -0.4 2,505,695 - Psyr_2148 0.78 -0.9 2,505,709 + Psyr_2148 0.80 +0.0 2,505,733 + Psyr_2148 0.83 -0.5 2,505,734 - Psyr_2148 0.83 -0.1 2,505,734 - Psyr_2148 0.83 +1.5 2,505,734 - Psyr_2148 0.83 -1.1 2,505,734 - Psyr_2148 0.83 -1.2 2,505,937 + Psyr_2149 0.32 +0.3 2,506,175 + -0.6 2,506,175 + -0.1 2,506,175 + +0.2 2,506,175 + +0.9 2,506,175 + +0.9 2,506,175 + +0.0 2,506,175 + -0.8 2,506,175 + -0.4 2,506,175 + +1.9 2,506,175 + +0.6 2,506,175 + +0.1 2,506,176 - +2.3 2,506,176 - +0.5 2,506,176 - -0.6 2,506,176 - -0.8 2,506,176 - +0.8 2,506,176 - +0.1 2,506,176 - -2.1 2,506,176 - -0.9 2,506,176 - +0.4 2,506,176 - -0.5 2,506,176 - -0.2 2,506,176 - +3.9 2,506,176 - +2.3 2,506,176 - +1.1 2,506,179 + +0.3 2,506,179 + +4.2 2,506,179 + +0.5 2,506,179 + +1.4 2,506,179 + +0.1 2,506,179 + +0.4 2,506,180 - +0.1 2,506,180 - +1.2 2,506,180 - -0.5 2,506,180 - -1.1 2,506,180 - -0.1 2,506,180 - +0.7 2,506,185 - -1.3 2,506,251 - +0.2 2,506,347 + Psyr_2150 0.45 -0.6 2,506,347 + Psyr_2150 0.45 +0.1 2,506,361 + Psyr_2150 0.52 -1.4 2,506,361 + Psyr_2150 0.52 +0.3 2,506,362 - Psyr_2150 0.52 -1.3 2,506,406 + Psyr_2150 0.72 -0.8 2,506,528 - +4.2 2,506,532 - +1.9 2,506,639 - -0.5 2,506,639 - +1.3 2,506,744 + +0.5 2,507,134 + Psyr_2151 0.49 -2.6 2,507,434 + Psyr_2151 0.80 +0.6
Or see this region's nucleotide sequence