Experiment: KB with Totarol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1936 and Psyr_1937 are separated by 111 nucleotides Psyr_1937 and Psyr_1938 are separated by 0 nucleotides Psyr_1938 and Psyr_1939 overlap by 4 nucleotides
Psyr_1936: Psyr_1936 - tRNA-U16,U17-dihydrouridine synthase, at 2,209,059 to 2,210,078
_1936
Psyr_1937: Psyr_1937 - UspA, at 2,210,190 to 2,210,621
_1937
Psyr_1938: Psyr_1938 - Response regulator receiver, at 2,210,622 to 2,211,086
_1938
Psyr_1939: Psyr_1939 - PAS, at 2,211,083 to 2,213,422
_1939
Position (kb)
2210
2211 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2209.206 kb on + strand, within Psyr_1936 at 2209.206 kb on + strand, within Psyr_1936 at 2209.536 kb on + strand, within Psyr_1936 at 2209.536 kb on + strand, within Psyr_1936 at 2209.665 kb on + strand, within Psyr_1936 at 2209.897 kb on - strand, within Psyr_1936 at 2209.949 kb on + strand, within Psyr_1936 at 2209.949 kb on + strand, within Psyr_1936 at 2209.950 kb on - strand at 2209.950 kb on - strand, within Psyr_1936 at 2209.950 kb on - strand, within Psyr_1936 at 2209.950 kb on - strand, within Psyr_1936 at 2210.014 kb on + strand at 2210.014 kb on + strand at 2210.014 kb on + strand at 2210.014 kb on + strand at 2210.014 kb on + strand at 2210.015 kb on - strand at 2210.015 kb on - strand at 2210.015 kb on - strand at 2210.015 kb on - strand at 2210.015 kb on - strand at 2210.015 kb on - strand at 2210.015 kb on - strand at 2210.015 kb on - strand at 2210.015 kb on - strand at 2210.015 kb on - strand at 2210.247 kb on + strand, within Psyr_1937 at 2210.248 kb on - strand, within Psyr_1937 at 2210.248 kb on - strand, within Psyr_1937 at 2210.248 kb on - strand, within Psyr_1937 at 2210.326 kb on - strand, within Psyr_1937 at 2210.619 kb on + strand at 2210.620 kb on - strand at 2210.696 kb on + strand, within Psyr_1938 at 2210.697 kb on - strand, within Psyr_1938 at 2210.697 kb on - strand, within Psyr_1938 at 2210.697 kb on - strand, within Psyr_1938 at 2210.698 kb on + strand, within Psyr_1938 at 2210.698 kb on + strand, within Psyr_1938 at 2210.699 kb on - strand, within Psyr_1938 at 2210.699 kb on - strand, within Psyr_1938 at 2210.699 kb on - strand, within Psyr_1938 at 2210.699 kb on - strand, within Psyr_1938 at 2210.848 kb on + strand, within Psyr_1938 at 2210.848 kb on + strand, within Psyr_1938 at 2210.849 kb on - strand, within Psyr_1938 at 2210.863 kb on - strand, within Psyr_1938 at 2211.603 kb on - strand, within Psyr_1939
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Totarol 10 mM remove 2,209,206 + Psyr_1936 0.14 +1.8 2,209,206 + Psyr_1936 0.14 +0.3 2,209,536 + Psyr_1936 0.47 -0.7 2,209,536 + Psyr_1936 0.47 +1.3 2,209,665 + Psyr_1936 0.59 -0.6 2,209,897 - Psyr_1936 0.82 -0.1 2,209,949 + Psyr_1936 0.87 +3.9 2,209,949 + Psyr_1936 0.87 -0.6 2,209,950 - +2.4 2,209,950 - Psyr_1936 0.87 +0.3 2,209,950 - Psyr_1936 0.87 +0.8 2,209,950 - Psyr_1936 0.87 -0.1 2,210,014 + +1.1 2,210,014 + +0.5 2,210,014 + +0.9 2,210,014 + -0.9 2,210,014 + +1.2 2,210,015 - +0.8 2,210,015 - -2.2 2,210,015 - +1.5 2,210,015 - -0.0 2,210,015 - -0.6 2,210,015 - -1.5 2,210,015 - +1.5 2,210,015 - +0.0 2,210,015 - +0.2 2,210,015 - +1.1 2,210,247 + Psyr_1937 0.13 -0.6 2,210,248 - Psyr_1937 0.13 +0.6 2,210,248 - Psyr_1937 0.13 -2.0 2,210,248 - Psyr_1937 0.13 -1.0 2,210,326 - Psyr_1937 0.31 -0.8 2,210,619 + -0.9 2,210,620 - +0.2 2,210,696 + Psyr_1938 0.16 +0.1 2,210,697 - Psyr_1938 0.16 +0.5 2,210,697 - Psyr_1938 0.16 +1.5 2,210,697 - Psyr_1938 0.16 +0.8 2,210,698 + Psyr_1938 0.16 -0.9 2,210,698 + Psyr_1938 0.16 -0.3 2,210,699 - Psyr_1938 0.17 -1.1 2,210,699 - Psyr_1938 0.17 -0.7 2,210,699 - Psyr_1938 0.17 -0.3 2,210,699 - Psyr_1938 0.17 +0.8 2,210,848 + Psyr_1938 0.49 -0.0 2,210,848 + Psyr_1938 0.49 -0.4 2,210,849 - Psyr_1938 0.49 -0.3 2,210,863 - Psyr_1938 0.52 +1.6 2,211,603 - Psyr_1939 0.22 +1.7
Or see this region's nucleotide sequence