Experiment: KB with Totarol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1016 and Psyr_1017 are separated by 113 nucleotides Psyr_1017 and Psyr_1018 overlap by 8 nucleotides Psyr_1018 and Psyr_1019 are separated by 43 nucleotides Psyr_1019 and Psyr_1020 overlap by 1 nucleotides
Psyr_1016: Psyr_1016 - Thioredoxin reductase, at 1,149,858 to 1,150,820
_1016
Psyr_1017: Psyr_1017 - type III effector HopJ1, at 1,150,934 to 1,151,275
_1017
Psyr_1018: Psyr_1018 - Protein of unknown function DUF1244, at 1,151,268 to 1,151,567
_1018
Psyr_1019: Psyr_1019 - Dihydroneopterin aldolase, at 1,151,611 to 1,151,982
_1019
Psyr_1020: Psyr_1020 - GTP cyclohydrolase I, at 1,151,982 to 1,152,545
_1020
Position (kb) 1150
1151
1152 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1150.810 kb on + strand at 1150.889 kb on + strand at 1150.890 kb on - strand at 1150.890 kb on - strand at 1150.890 kb on - strand at 1150.890 kb on - strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.891 kb on + strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1150.892 kb on - strand at 1151.190 kb on + strand, within Psyr_1017 at 1151.190 kb on + strand, within Psyr_1017 at 1151.353 kb on + strand, within Psyr_1018 at 1151.366 kb on - strand, within Psyr_1018 at 1151.366 kb on - strand, within Psyr_1018 at 1151.428 kb on + strand, within Psyr_1018 at 1151.428 kb on + strand, within Psyr_1018 at 1151.428 kb on + strand, within Psyr_1018 at 1151.429 kb on - strand, within Psyr_1018 at 1151.429 kb on - strand, within Psyr_1018 at 1151.429 kb on - strand, within Psyr_1018 at 1151.603 kb on + strand at 1151.604 kb on - strand at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.690 kb on + strand, within Psyr_1019 at 1151.691 kb on - strand, within Psyr_1019 at 1151.691 kb on - strand, within Psyr_1019 at 1151.691 kb on - strand, within Psyr_1019 at 1151.691 kb on - strand, within Psyr_1019 at 1151.805 kb on - strand, within Psyr_1019 at 1151.805 kb on - strand, within Psyr_1019 at 1151.856 kb on - strand, within Psyr_1019 at 1151.856 kb on - strand, within Psyr_1019 at 1151.983 kb on + strand at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.065 kb on + strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.066 kb on - strand, within Psyr_1020 at 1152.139 kb on - strand, within Psyr_1020 at 1152.139 kb on - strand, within Psyr_1020
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Totarol 10 mM remove 1,150,810 + +3.2 1,150,889 + +0.9 1,150,890 - -1.1 1,150,890 - -0.4 1,150,890 - -0.1 1,150,890 - +0.3 1,150,891 + +0.6 1,150,891 + -0.8 1,150,891 + -1.3 1,150,891 + -0.1 1,150,891 + +0.8 1,150,891 + -0.7 1,150,891 + -2.4 1,150,891 + -2.1 1,150,891 + -2.4 1,150,892 - +0.1 1,150,892 - +0.2 1,150,892 - +0.5 1,150,892 - -0.1 1,150,892 - -0.1 1,150,892 - +0.2 1,151,190 + Psyr_1017 0.75 -3.0 1,151,190 + Psyr_1017 0.75 -0.3 1,151,353 + Psyr_1018 0.28 +0.6 1,151,366 - Psyr_1018 0.33 -0.5 1,151,366 - Psyr_1018 0.33 +0.2 1,151,428 + Psyr_1018 0.53 -0.5 1,151,428 + Psyr_1018 0.53 -2.1 1,151,428 + Psyr_1018 0.53 -0.0 1,151,429 - Psyr_1018 0.54 +0.5 1,151,429 - Psyr_1018 0.54 -2.6 1,151,429 - Psyr_1018 0.54 -0.9 1,151,603 + -0.1 1,151,604 - +0.7 1,151,690 + Psyr_1019 0.21 -0.8 1,151,690 + Psyr_1019 0.21 -0.7 1,151,690 + Psyr_1019 0.21 +0.9 1,151,690 + Psyr_1019 0.21 -0.2 1,151,690 + Psyr_1019 0.21 +0.6 1,151,690 + Psyr_1019 0.21 +0.4 1,151,691 - Psyr_1019 0.22 +0.5 1,151,691 - Psyr_1019 0.22 +1.6 1,151,691 - Psyr_1019 0.22 +0.6 1,151,691 - Psyr_1019 0.22 +1.2 1,151,805 - Psyr_1019 0.52 +0.1 1,151,805 - Psyr_1019 0.52 -1.2 1,151,856 - Psyr_1019 0.66 -2.2 1,151,856 - Psyr_1019 0.66 +2.6 1,151,983 + -0.2 1,152,065 + Psyr_1020 0.15 -0.1 1,152,065 + Psyr_1020 0.15 +0.4 1,152,065 + Psyr_1020 0.15 -1.0 1,152,065 + Psyr_1020 0.15 -0.1 1,152,065 + Psyr_1020 0.15 -0.5 1,152,065 + Psyr_1020 0.15 -0.3 1,152,065 + Psyr_1020 0.15 +1.5 1,152,066 - Psyr_1020 0.15 -1.0 1,152,066 - Psyr_1020 0.15 +1.0 1,152,066 - Psyr_1020 0.15 -1.3 1,152,066 - Psyr_1020 0.15 +1.4 1,152,066 - Psyr_1020 0.15 +0.0 1,152,066 - Psyr_1020 0.15 +3.4 1,152,066 - Psyr_1020 0.15 +0.1 1,152,066 - Psyr_1020 0.15 -0.2 1,152,066 - Psyr_1020 0.15 +0.1 1,152,066 - Psyr_1020 0.15 -0.1 1,152,066 - Psyr_1020 0.15 -0.4 1,152,066 - Psyr_1020 0.15 -1.7 1,152,139 - Psyr_1020 0.28 +0.1 1,152,139 - Psyr_1020 0.28 -0.2
Or see this region's nucleotide sequence