Experiment: KB with Totarol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0025 and Psyr_0026 are separated by 184 nucleotides Psyr_0026 and Psyr_0027 are separated by 71 nucleotides Psyr_0027 and Psyr_0028 are separated by 166 nucleotides
Psyr_0025: Psyr_0025 - shikimate dehydrogenase, at 28,635 to 29,459
_0025
Psyr_0026: Psyr_0026 - Sulfate transporter/antisigma-factor antagonist STAS:Sulphate transporter, at 29,644 to 31,212
_0026
Psyr_0027: Psyr_0027 - conserved hypothetical protein, at 31,284 to 31,601
_0027
Psyr_0028: Psyr_0028 - Substrate-binding region of ABC-type glycine betaine transport system, at 31,768 to 32,694
_0028
Position (kb)
29
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 29.088 kb on - strand, within Psyr_0025 at 29.088 kb on - strand, within Psyr_0025 at 29.140 kb on + strand, within Psyr_0025 at 29.269 kb on - strand, within Psyr_0025 at 29.280 kb on + strand, within Psyr_0025 at 29.280 kb on + strand, within Psyr_0025 at 29.280 kb on + strand, within Psyr_0025 at 29.280 kb on + strand, within Psyr_0025 at 29.280 kb on + strand, within Psyr_0025 at 29.281 kb on - strand, within Psyr_0025 at 29.281 kb on - strand, within Psyr_0025 at 29.466 kb on - strand at 29.497 kb on + strand at 29.497 kb on + strand at 29.497 kb on + strand at 29.497 kb on + strand at 29.497 kb on + strand at 29.498 kb on - strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.499 kb on + strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.500 kb on - strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.503 kb on + strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.504 kb on - strand at 29.610 kb on + strand at 29.610 kb on + strand at 29.610 kb on + strand at 29.611 kb on - strand at 29.611 kb on - strand at 29.611 kb on - strand at 29.638 kb on + strand at 29.638 kb on + strand at 29.638 kb on + strand at 29.638 kb on + strand at 29.639 kb on - strand at 29.639 kb on - strand at 29.639 kb on - strand at 29.639 kb on - strand at 29.639 kb on - strand at 29.792 kb on + strand at 29.792 kb on + strand at 29.845 kb on - strand, within Psyr_0026 at 29.868 kb on - strand, within Psyr_0026 at 29.868 kb on - strand, within Psyr_0026 at 29.966 kb on + strand, within Psyr_0026 at 29.967 kb on - strand, within Psyr_0026 at 29.986 kb on + strand, within Psyr_0026 at 29.986 kb on + strand, within Psyr_0026 at 29.986 kb on + strand, within Psyr_0026 at 29.987 kb on - strand, within Psyr_0026 at 29.996 kb on + strand, within Psyr_0026 at 29.996 kb on + strand, within Psyr_0026 at 29.997 kb on - strand, within Psyr_0026 at 29.997 kb on - strand, within Psyr_0026 at 30.248 kb on + strand, within Psyr_0026 at 30.248 kb on + strand, within Psyr_0026 at 30.248 kb on + strand, within Psyr_0026 at 30.905 kb on + strand, within Psyr_0026 at 30.905 kb on + strand, within Psyr_0026 at 30.905 kb on + strand, within Psyr_0026 at 30.905 kb on + strand, within Psyr_0026 at 30.905 kb on + strand, within Psyr_0026 at 30.905 kb on + strand, within Psyr_0026 at 30.905 kb on + strand, within Psyr_0026 at 30.905 kb on + strand, within Psyr_0026 at 30.905 kb on + strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.906 kb on - strand, within Psyr_0026 at 30.947 kb on + strand, within Psyr_0026 at 30.947 kb on + strand, within Psyr_0026 at 30.947 kb on + strand, within Psyr_0026 at 30.947 kb on + strand, within Psyr_0026 at 30.948 kb on - strand, within Psyr_0026 at 30.948 kb on - strand, within Psyr_0026 at 30.961 kb on + strand, within Psyr_0026 at 30.962 kb on - strand, within Psyr_0026 at 30.962 kb on - strand, within Psyr_0026 at 30.963 kb on + strand, within Psyr_0026 at 30.963 kb on + strand, within Psyr_0026 at 30.963 kb on + strand, within Psyr_0026 at 30.963 kb on + strand, within Psyr_0026 at 30.963 kb on + strand, within Psyr_0026 at 30.963 kb on + strand, within Psyr_0026 at 30.964 kb on - strand, within Psyr_0026 at 31.041 kb on - strand, within Psyr_0026 at 31.041 kb on - strand, within Psyr_0026 at 31.041 kb on - strand, within Psyr_0026 at 31.041 kb on - strand, within Psyr_0026 at 31.041 kb on - strand, within Psyr_0026 at 31.049 kb on + strand, within Psyr_0026 at 31.079 kb on + strand at 31.079 kb on + strand at 31.080 kb on - strand at 31.080 kb on - strand at 31.285 kb on + strand at 31.285 kb on + strand at 31.285 kb on + strand at 31.286 kb on - strand at 31.286 kb on - strand at 31.286 kb on - strand at 31.286 kb on - strand at 31.322 kb on + strand, within Psyr_0027 at 31.322 kb on + strand, within Psyr_0027 at 31.323 kb on - strand, within Psyr_0027 at 31.323 kb on - strand, within Psyr_0027 at 31.323 kb on - strand, within Psyr_0027 at 31.358 kb on + strand, within Psyr_0027 at 31.359 kb on - strand, within Psyr_0027 at 31.359 kb on - strand, within Psyr_0027 at 31.359 kb on - strand, within Psyr_0027 at 31.359 kb on - strand, within Psyr_0027 at 31.359 kb on - strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.363 kb on + strand, within Psyr_0027 at 31.364 kb on - strand, within Psyr_0027 at 31.364 kb on - strand, within Psyr_0027 at 31.364 kb on - strand, within Psyr_0027 at 31.364 kb on - strand, within Psyr_0027 at 31.364 kb on - strand, within Psyr_0027 at 31.364 kb on - strand, within Psyr_0027 at 31.364 kb on - strand, within Psyr_0027 at 31.364 kb on - strand, within Psyr_0027 at 31.364 kb on - strand, within Psyr_0027 at 31.411 kb on + strand, within Psyr_0027 at 31.411 kb on + strand, within Psyr_0027 at 31.411 kb on + strand, within Psyr_0027 at 31.412 kb on - strand, within Psyr_0027 at 31.412 kb on - strand, within Psyr_0027 at 31.412 kb on - strand, within Psyr_0027 at 31.412 kb on - strand, within Psyr_0027 at 31.454 kb on + strand, within Psyr_0027 at 31.455 kb on - strand, within Psyr_0027 at 31.455 kb on - strand, within Psyr_0027 at 31.584 kb on + strand at 31.584 kb on + strand at 31.584 kb on + strand at 31.585 kb on - strand at 31.585 kb on - strand at 31.585 kb on - strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.586 kb on + strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.587 kb on - strand at 31.617 kb on + strand at 31.642 kb on - strand at 31.676 kb on + strand at 31.676 kb on + strand at 31.676 kb on + strand at 31.677 kb on - strand at 31.705 kb on + strand at 31.705 kb on + strand at 31.705 kb on + strand at 31.706 kb on - strand at 31.706 kb on - strand at 31.706 kb on - strand at 31.706 kb on - strand at 31.752 kb on + strand at 31.752 kb on + strand at 31.752 kb on + strand at 31.752 kb on + strand at 31.752 kb on + strand at 31.753 kb on - strand at 31.753 kb on - strand at 31.753 kb on - strand at 31.753 kb on - strand at 31.753 kb on - strand at 31.753 kb on - strand at 31.753 kb on - strand at 31.753 kb on - strand at 31.753 kb on - strand at 31.756 kb on + strand at 31.757 kb on - strand at 31.769 kb on + strand at 31.770 kb on - strand at 31.992 kb on - strand, within Psyr_0028 at 31.992 kb on - strand, within Psyr_0028 at 32.011 kb on - strand, within Psyr_0028 at 32.011 kb on - strand, within Psyr_0028 at 32.036 kb on + strand, within Psyr_0028 at 32.037 kb on - strand, within Psyr_0028 at 32.037 kb on - strand, within Psyr_0028 at 32.037 kb on - strand, within Psyr_0028 at 32.057 kb on + strand, within Psyr_0028 at 32.057 kb on + strand, within Psyr_0028 at 32.057 kb on + strand, within Psyr_0028 at 32.058 kb on - strand, within Psyr_0028 at 32.058 kb on - strand, within Psyr_0028 at 32.058 kb on - strand, within Psyr_0028
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Totarol 10 mM remove 29,088 - Psyr_0025 0.55 -1.1 29,088 - Psyr_0025 0.55 +0.2 29,140 + Psyr_0025 0.61 +0.6 29,269 - Psyr_0025 0.77 +1.0 29,280 + Psyr_0025 0.78 +0.9 29,280 + Psyr_0025 0.78 +0.0 29,280 + Psyr_0025 0.78 +0.1 29,280 + Psyr_0025 0.78 -0.7 29,280 + Psyr_0025 0.78 -1.7 29,281 - Psyr_0025 0.78 -0.1 29,281 - Psyr_0025 0.78 +1.3 29,466 - -0.1 29,497 + +0.6 29,497 + -0.1 29,497 + -0.1 29,497 + +0.1 29,497 + -1.0 29,498 - -0.8 29,499 + +0.7 29,499 + +1.6 29,499 + -0.2 29,499 + -1.3 29,499 + +0.9 29,499 + +1.3 29,499 + -0.6 29,499 + +0.3 29,499 + -0.5 29,499 + +0.1 29,499 + +0.1 29,499 + -0.0 29,499 + -1.4 29,499 + +0.2 29,499 + +0.5 29,499 + +0.1 29,499 + -0.8 29,500 - -0.1 29,500 - -0.3 29,500 - -1.0 29,500 - -1.0 29,500 - +1.3 29,500 - -0.6 29,500 - -0.1 29,500 - -0.7 29,500 - +1.5 29,500 - -1.7 29,500 - +0.6 29,500 - +0.1 29,500 - +1.3 29,503 + +1.3 29,503 + -0.4 29,503 + -0.1 29,503 + -0.5 29,503 + -1.0 29,503 + -0.6 29,503 + +1.0 29,503 + -0.5 29,503 + +0.0 29,503 + +0.0 29,503 + -0.1 29,503 + -0.1 29,503 + -0.3 29,503 + +0.1 29,503 + +0.1 29,503 + +1.1 29,503 + +0.3 29,503 + -0.3 29,503 + -1.7 29,503 + -1.1 29,503 + +0.4 29,503 + +1.0 29,504 - +0.3 29,504 - +1.5 29,504 - -1.2 29,504 - +0.7 29,504 - +1.5 29,504 - +1.8 29,504 - +0.5 29,504 - +0.3 29,504 - +0.8 29,504 - +0.4 29,504 - -1.6 29,504 - -0.6 29,504 - -0.3 29,610 + +1.1 29,610 + -1.1 29,610 + -1.0 29,611 - +0.6 29,611 - -1.1 29,611 - -0.2 29,638 + -0.4 29,638 + -0.6 29,638 + +0.3 29,638 + -0.0 29,639 - -1.1 29,639 - +0.7 29,639 - +1.6 29,639 - +0.5 29,639 - +0.6 29,792 + -0.8 29,792 + -0.7 29,845 - Psyr_0026 0.13 +1.0 29,868 - Psyr_0026 0.14 -0.5 29,868 - Psyr_0026 0.14 -1.4 29,966 + Psyr_0026 0.21 -2.4 29,967 - Psyr_0026 0.21 -0.1 29,986 + Psyr_0026 0.22 -0.7 29,986 + Psyr_0026 0.22 -0.6 29,986 + Psyr_0026 0.22 +0.6 29,987 - Psyr_0026 0.22 -0.6 29,996 + Psyr_0026 0.22 +0.2 29,996 + Psyr_0026 0.22 +0.1 29,997 - Psyr_0026 0.22 +1.4 29,997 - Psyr_0026 0.22 +0.7 30,248 + Psyr_0026 0.38 +0.6 30,248 + Psyr_0026 0.38 -1.4 30,248 + Psyr_0026 0.38 -0.8 30,905 + Psyr_0026 0.80 -0.5 30,905 + Psyr_0026 0.80 -0.7 30,905 + Psyr_0026 0.80 -1.2 30,905 + Psyr_0026 0.80 -0.1 30,905 + Psyr_0026 0.80 -0.1 30,905 + Psyr_0026 0.80 +0.4 30,905 + Psyr_0026 0.80 -0.4 30,905 + Psyr_0026 0.80 -1.6 30,905 + Psyr_0026 0.80 -0.1 30,906 - Psyr_0026 0.80 +0.6 30,906 - Psyr_0026 0.80 +1.3 30,906 - Psyr_0026 0.80 -0.2 30,906 - Psyr_0026 0.80 -1.8 30,906 - Psyr_0026 0.80 -0.6 30,906 - Psyr_0026 0.80 -1.6 30,906 - Psyr_0026 0.80 -1.0 30,906 - Psyr_0026 0.80 +0.1 30,906 - Psyr_0026 0.80 +0.3 30,906 - Psyr_0026 0.80 +1.0 30,906 - Psyr_0026 0.80 -0.3 30,906 - Psyr_0026 0.80 -1.3 30,906 - Psyr_0026 0.80 -2.6 30,947 + Psyr_0026 0.83 +1.3 30,947 + Psyr_0026 0.83 +0.3 30,947 + Psyr_0026 0.83 +0.2 30,947 + Psyr_0026 0.83 +0.8 30,948 - Psyr_0026 0.83 +4.6 30,948 - Psyr_0026 0.83 -0.4 30,961 + Psyr_0026 0.84 -0.7 30,962 - Psyr_0026 0.84 +0.7 30,962 - Psyr_0026 0.84 +0.1 30,963 + Psyr_0026 0.84 -1.3 30,963 + Psyr_0026 0.84 -1.4 30,963 + Psyr_0026 0.84 -1.6 30,963 + Psyr_0026 0.84 +0.5 30,963 + Psyr_0026 0.84 -0.2 30,963 + Psyr_0026 0.84 -1.3 30,964 - Psyr_0026 0.84 -0.3 31,041 - Psyr_0026 0.89 -1.8 31,041 - Psyr_0026 0.89 -0.4 31,041 - Psyr_0026 0.89 -1.0 31,041 - Psyr_0026 0.89 +2.1 31,041 - Psyr_0026 0.89 +0.1 31,049 + Psyr_0026 0.90 -0.0 31,079 + -0.3 31,079 + +0.5 31,080 - +0.0 31,080 - -0.5 31,285 + -0.6 31,285 + -1.2 31,285 + -0.1 31,286 - +0.2 31,286 - +0.3 31,286 - -0.8 31,286 - -1.3 31,322 + Psyr_0027 0.12 -0.1 31,322 + Psyr_0027 0.12 +0.1 31,323 - Psyr_0027 0.12 +0.9 31,323 - Psyr_0027 0.12 +1.0 31,323 - Psyr_0027 0.12 -0.6 31,358 + Psyr_0027 0.23 +0.1 31,359 - Psyr_0027 0.24 +0.6 31,359 - Psyr_0027 0.24 +2.9 31,359 - Psyr_0027 0.24 +0.9 31,359 - Psyr_0027 0.24 +0.5 31,359 - Psyr_0027 0.24 -0.1 31,363 + Psyr_0027 0.25 +0.8 31,363 + Psyr_0027 0.25 -0.5 31,363 + Psyr_0027 0.25 -1.6 31,363 + Psyr_0027 0.25 +0.3 31,363 + Psyr_0027 0.25 +0.5 31,363 + Psyr_0027 0.25 -0.3 31,363 + Psyr_0027 0.25 -2.4 31,363 + Psyr_0027 0.25 -0.2 31,363 + Psyr_0027 0.25 +0.1 31,363 + Psyr_0027 0.25 -0.6 31,364 - Psyr_0027 0.25 +1.3 31,364 - Psyr_0027 0.25 -0.8 31,364 - Psyr_0027 0.25 -1.8 31,364 - Psyr_0027 0.25 +0.0 31,364 - Psyr_0027 0.25 +0.9 31,364 - Psyr_0027 0.25 +0.5 31,364 - Psyr_0027 0.25 +0.1 31,364 - Psyr_0027 0.25 +0.6 31,364 - Psyr_0027 0.25 +0.5 31,411 + Psyr_0027 0.40 -0.4 31,411 + Psyr_0027 0.40 +1.9 31,411 + Psyr_0027 0.40 -1.3 31,412 - Psyr_0027 0.40 +0.0 31,412 - Psyr_0027 0.40 -1.8 31,412 - Psyr_0027 0.40 +0.9 31,412 - Psyr_0027 0.40 -0.2 31,454 + Psyr_0027 0.53 +0.4 31,455 - Psyr_0027 0.54 -1.0 31,455 - Psyr_0027 0.54 -0.3 31,584 + -0.3 31,584 + +0.7 31,584 + -0.4 31,585 - -0.9 31,585 - -1.5 31,585 - -0.8 31,586 + -0.6 31,586 + -0.7 31,586 + -0.1 31,586 + -0.2 31,586 + -0.6 31,586 + +0.7 31,586 + +2.2 31,586 + +0.1 31,586 + -0.5 31,586 + -0.1 31,586 + +0.5 31,586 + +1.9 31,586 + +0.6 31,586 + -0.7 31,586 + -1.1 31,586 + -1.2 31,587 - +0.0 31,587 - +1.8 31,587 - +0.8 31,587 - +0.0 31,587 - -0.5 31,587 - -1.4 31,587 - +0.0 31,587 - -0.7 31,587 - +2.5 31,587 - -0.4 31,587 - +0.6 31,587 - -0.1 31,587 - +0.0 31,587 - +0.0 31,587 - +1.9 31,587 - +1.1 31,587 - -0.0 31,587 - -1.1 31,587 - +2.2 31,587 - +0.0 31,587 - -1.4 31,587 - +1.6 31,587 - +0.1 31,617 + -1.8 31,642 - +0.0 31,676 + -0.7 31,676 + -2.0 31,676 + +0.0 31,677 - -0.3 31,705 + +2.2 31,705 + +1.3 31,705 + +0.5 31,706 - -0.7 31,706 - -0.1 31,706 - -0.2 31,706 - -0.2 31,752 + +0.2 31,752 + +2.2 31,752 + -0.8 31,752 + -1.6 31,752 + +2.1 31,753 - -0.2 31,753 - +0.3 31,753 - -0.7 31,753 - +1.9 31,753 - +2.1 31,753 - +1.6 31,753 - +0.2 31,753 - -0.2 31,753 - -0.4 31,756 + -2.0 31,757 - -0.5 31,769 + -0.2 31,770 - +0.3 31,992 - Psyr_0028 0.24 -1.3 31,992 - Psyr_0028 0.24 -0.8 32,011 - Psyr_0028 0.26 -1.8 32,011 - Psyr_0028 0.26 -0.2 32,036 + Psyr_0028 0.29 +0.7 32,037 - Psyr_0028 0.29 +0.0 32,037 - Psyr_0028 0.29 -0.2 32,037 - Psyr_0028 0.29 -0.7 32,057 + Psyr_0028 0.31 -1.0 32,057 + Psyr_0028 0.31 +0.7 32,057 + Psyr_0028 0.31 -0.6 32,058 - Psyr_0028 0.31 -0.5 32,058 - Psyr_0028 0.31 -2.3 32,058 - Psyr_0028 0.31 +1.2
Or see this region's nucleotide sequence