Experiment: BHIS with Sulfamonomethoxine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Thr and BT4566 are separated by 68 nucleotides BT4566 and BT4567 are separated by 1 nucleotides BT4567 and BT4568 are separated by 24 nucleotides
BT_t69: tRNA-Thr - tRNA-Thr (RefSeq), at 5,986,401 to 5,986,474
tRNA-Thr
BT4566: BT4566 - two-component system response regulator protein (NCBI ptt file), at 5,986,543 to 5,987,247
BT4566
BT4567: BT4567 - putative two-component system sensor but no kinase domain (NCBI ptt file), at 5,987,249 to 5,988,289
BT4567
BT4568: BT4568 - conserved hypothetical protein (NCBI ptt file), at 5,988,314 to 5,990,749
BT4568
Position (kb)
5987
5988
5989 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5986.522 kb on + strand at 5986.523 kb on - strand at 5986.587 kb on + strand at 5986.683 kb on - strand, within BT4566 at 5986.744 kb on - strand, within BT4566 at 5986.748 kb on + strand, within BT4566 at 5986.748 kb on + strand, within BT4566 at 5986.749 kb on - strand, within BT4566 at 5986.754 kb on - strand, within BT4566 at 5986.754 kb on - strand, within BT4566 at 5986.942 kb on + strand, within BT4566 at 5986.952 kb on - strand, within BT4566 at 5986.958 kb on + strand, within BT4566 at 5986.966 kb on + strand, within BT4566 at 5987.004 kb on - strand, within BT4566 at 5987.004 kb on - strand, within BT4566 at 5987.044 kb on - strand, within BT4566 at 5987.057 kb on - strand, within BT4566 at 5987.101 kb on + strand, within BT4566 at 5987.124 kb on + strand, within BT4566 at 5987.125 kb on - strand, within BT4566 at 5987.226 kb on + strand at 5987.233 kb on - strand at 5987.284 kb on + strand at 5987.285 kb on - strand at 5987.484 kb on + strand, within BT4567 at 5987.572 kb on + strand, within BT4567 at 5987.610 kb on + strand, within BT4567 at 5987.611 kb on - strand, within BT4567 at 5987.611 kb on - strand, within BT4567 at 5987.657 kb on + strand, within BT4567 at 5987.750 kb on - strand, within BT4567 at 5987.892 kb on - strand, within BT4567 at 5987.892 kb on - strand, within BT4567 at 5987.896 kb on - strand, within BT4567 at 5987.900 kb on + strand, within BT4567 at 5987.901 kb on - strand, within BT4567 at 5987.983 kb on + strand, within BT4567 at 5987.984 kb on - strand, within BT4567 at 5987.992 kb on + strand, within BT4567 at 5987.992 kb on + strand, within BT4567 at 5988.036 kb on - strand, within BT4567 at 5988.036 kb on - strand, within BT4567 at 5988.116 kb on + strand, within BT4567 at 5988.117 kb on - strand, within BT4567 at 5988.216 kb on - strand at 5988.221 kb on - strand at 5988.253 kb on + strand at 5988.254 kb on - strand at 5988.258 kb on - strand at 5988.276 kb on + strand at 5988.291 kb on - strand at 5988.341 kb on + strand at 5988.342 kb on - strand at 5988.342 kb on - strand at 5988.466 kb on + strand at 5988.466 kb on + strand at 5988.494 kb on + strand at 5988.560 kb on + strand, within BT4568 at 5988.570 kb on - strand, within BT4568 at 5988.647 kb on + strand, within BT4568 at 5988.652 kb on + strand, within BT4568 at 5988.827 kb on + strand, within BT4568 at 5988.907 kb on - strand, within BT4568 at 5988.912 kb on - strand, within BT4568 at 5988.958 kb on + strand, within BT4568 at 5988.958 kb on + strand, within BT4568 at 5988.959 kb on - strand, within BT4568 at 5988.962 kb on + strand, within BT4568 at 5989.001 kb on + strand, within BT4568 at 5989.037 kb on - strand, within BT4568 at 5989.079 kb on - strand, within BT4568 at 5989.108 kb on - strand, within BT4568 at 5989.136 kb on + strand, within BT4568 at 5989.137 kb on - strand, within BT4568 at 5989.140 kb on + strand, within BT4568 at 5989.140 kb on + strand, within BT4568 at 5989.141 kb on - strand, within BT4568 at 5989.199 kb on - strand, within BT4568 at 5989.199 kb on - strand, within BT4568 at 5989.211 kb on + strand, within BT4568 at 5989.222 kb on - strand, within BT4568 at 5989.272 kb on + strand, within BT4568 at 5989.273 kb on - strand, within BT4568
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfamonomethoxine 0.2 mM remove 5,986,522 + -0.9 5,986,523 - +2.1 5,986,587 + -2.9 5,986,683 - BT4566 0.20 -1.5 5,986,744 - BT4566 0.29 -1.5 5,986,748 + BT4566 0.29 -2.7 5,986,748 + BT4566 0.29 +0.1 5,986,749 - BT4566 0.29 +1.7 5,986,754 - BT4566 0.30 -1.5 5,986,754 - BT4566 0.30 +0.2 5,986,942 + BT4566 0.57 +0.7 5,986,952 - BT4566 0.58 -1.9 5,986,958 + BT4566 0.59 -2.4 5,986,966 + BT4566 0.60 -0.9 5,987,004 - BT4566 0.65 -1.4 5,987,004 - BT4566 0.65 -2.7 5,987,044 - BT4566 0.71 -1.2 5,987,057 - BT4566 0.73 -1.3 5,987,101 + BT4566 0.79 -2.7 5,987,124 + BT4566 0.82 -0.5 5,987,125 - BT4566 0.83 -1.8 5,987,226 + -0.9 5,987,233 - +3.1 5,987,284 + -1.9 5,987,285 - -3.1 5,987,484 + BT4567 0.23 +1.7 5,987,572 + BT4567 0.31 +0.1 5,987,610 + BT4567 0.35 +0.1 5,987,611 - BT4567 0.35 -0.9 5,987,611 - BT4567 0.35 -0.5 5,987,657 + BT4567 0.39 -0.9 5,987,750 - BT4567 0.48 -0.5 5,987,892 - BT4567 0.62 +0.1 5,987,892 - BT4567 0.62 +0.1 5,987,896 - BT4567 0.62 +1.1 5,987,900 + BT4567 0.63 +2.0 5,987,901 - BT4567 0.63 -0.5 5,987,983 + BT4567 0.71 -0.5 5,987,984 - BT4567 0.71 -1.9 5,987,992 + BT4567 0.71 -2.9 5,987,992 + BT4567 0.71 -0.5 5,988,036 - BT4567 0.76 +0.1 5,988,036 - BT4567 0.76 +0.1 5,988,116 + BT4567 0.83 -1.2 5,988,117 - BT4567 0.83 +2.4 5,988,216 - +1.7 5,988,221 - +1.2 5,988,253 + +0.1 5,988,254 - -3.3 5,988,258 - -0.6 5,988,276 + +2.1 5,988,291 - -2.2 5,988,341 + +1.1 5,988,342 - -0.9 5,988,342 - -3.6 5,988,466 + +2.1 5,988,466 + +2.9 5,988,494 + -0.3 5,988,560 + BT4568 0.10 -2.5 5,988,570 - BT4568 0.11 +0.1 5,988,647 + BT4568 0.14 -0.9 5,988,652 + BT4568 0.14 +0.1 5,988,827 + BT4568 0.21 -4.9 5,988,907 - BT4568 0.24 -2.1 5,988,912 - BT4568 0.25 +0.1 5,988,958 + BT4568 0.26 +0.7 5,988,958 + BT4568 0.26 -2.0 5,988,959 - BT4568 0.26 -2.2 5,988,962 + BT4568 0.27 -2.5 5,989,001 + BT4568 0.28 -1.1 5,989,037 - BT4568 0.30 -1.8 5,989,079 - BT4568 0.31 +1.7 5,989,108 - BT4568 0.33 +1.7 5,989,136 + BT4568 0.34 -1.5 5,989,137 - BT4568 0.34 -0.9 5,989,140 + BT4568 0.34 -1.5 5,989,140 + BT4568 0.34 +0.1 5,989,141 - BT4568 0.34 +0.1 5,989,199 - BT4568 0.36 +0.1 5,989,199 - BT4568 0.36 +0.8 5,989,211 + BT4568 0.37 -0.4 5,989,222 - BT4568 0.37 +0.1 5,989,272 + BT4568 0.39 +1.1 5,989,273 - BT4568 0.39 -1.6
Or see this region's nucleotide sequence