Experiment: BHIS with Sulfamonomethoxine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4526 and BT4527 are separated by 15 nucleotides BT4527 and BT4528 are separated by 15 nucleotides BT4528 and BT4529 are separated by 441 nucleotides BT4529 and BT4530 are separated by 5 nucleotides
BT4526: BT4526 - conserved hypothetical protein (NCBI ptt file), at 5,944,651 to 5,945,418
BT4526
BT4527: BT4527 - hypothetical protein (NCBI ptt file), at 5,945,434 to 5,945,976
BT4527
BT4528: BT4528 - conserved hypothetical protein (NCBI ptt file), at 5,945,992 to 5,946,576
BT4528
BT4529: BT4529 - hypothetical protein (NCBI ptt file), at 5,947,018 to 5,947,287
BT4529
BT4530: BT4530 - ATP-dependent DNA helicase recG (NCBI ptt file), at 5,947,293 to 5,948,714
BT4530
Position (kb)
5945
5946
5947 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5945.010 kb on + strand, within BT4526 at 5945.011 kb on - strand, within BT4526 at 5945.013 kb on - strand, within BT4526 at 5945.059 kb on + strand, within BT4526 at 5945.143 kb on + strand, within BT4526 at 5945.143 kb on + strand, within BT4526 at 5945.144 kb on - strand, within BT4526 at 5945.305 kb on + strand, within BT4526 at 5945.406 kb on - strand at 5945.410 kb on + strand at 5945.418 kb on + strand at 5945.462 kb on + strand at 5945.485 kb on + strand at 5945.517 kb on - strand, within BT4527 at 5945.600 kb on + strand, within BT4527 at 5945.626 kb on - strand, within BT4527 at 5945.626 kb on - strand, within BT4527 at 5945.739 kb on + strand, within BT4527 at 5945.793 kb on + strand, within BT4527 at 5945.793 kb on + strand, within BT4527 at 5945.793 kb on + strand, within BT4527 at 5945.794 kb on - strand, within BT4527 at 5945.998 kb on - strand at 5946.032 kb on - strand at 5946.080 kb on - strand, within BT4528 at 5946.092 kb on + strand, within BT4528 at 5946.092 kb on + strand, within BT4528 at 5946.169 kb on + strand, within BT4528 at 5946.169 kb on + strand, within BT4528 at 5946.186 kb on + strand, within BT4528 at 5946.228 kb on - strand, within BT4528 at 5946.228 kb on - strand, within BT4528 at 5946.229 kb on + strand, within BT4528 at 5946.230 kb on - strand, within BT4528 at 5946.302 kb on - strand, within BT4528 at 5946.304 kb on - strand, within BT4528 at 5946.327 kb on + strand, within BT4528 at 5946.436 kb on - strand, within BT4528 at 5946.454 kb on + strand, within BT4528 at 5946.456 kb on + strand, within BT4528 at 5946.483 kb on + strand, within BT4528 at 5946.541 kb on - strand at 5946.553 kb on - strand at 5946.568 kb on - strand at 5946.749 kb on - strand at 5946.749 kb on - strand at 5946.756 kb on + strand at 5946.756 kb on + strand at 5946.757 kb on - strand at 5946.769 kb on - strand at 5946.875 kb on + strand at 5946.876 kb on - strand at 5946.991 kb on + strand at 5946.992 kb on - strand at 5946.996 kb on + strand at 5946.998 kb on + strand at 5946.999 kb on - strand at 5947.034 kb on + strand at 5947.098 kb on + strand, within BT4529 at 5947.099 kb on - strand, within BT4529 at 5947.101 kb on - strand, within BT4529 at 5947.109 kb on - strand, within BT4529 at 5947.113 kb on - strand, within BT4529 at 5947.297 kb on - strand at 5947.315 kb on + strand at 5947.316 kb on - strand at 5947.350 kb on - strand at 5947.385 kb on + strand at 5947.386 kb on - strand at 5947.434 kb on - strand at 5947.442 kb on + strand, within BT4530 at 5947.548 kb on + strand, within BT4530 at 5947.549 kb on - strand, within BT4530 at 5947.560 kb on - strand, within BT4530
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfamonomethoxine 0.2 mM remove 5,945,010 + BT4526 0.47 +0.1 5,945,011 - BT4526 0.47 -0.9 5,945,013 - BT4526 0.47 +1.1 5,945,059 + BT4526 0.53 +0.1 5,945,143 + BT4526 0.64 -2.4 5,945,143 + BT4526 0.64 -1.4 5,945,144 - BT4526 0.64 -2.3 5,945,305 + BT4526 0.85 -2.7 5,945,406 - -0.9 5,945,410 + +0.6 5,945,418 + -0.9 5,945,462 + -1.9 5,945,485 + -2.9 5,945,517 - BT4527 0.15 -1.4 5,945,600 + BT4527 0.31 +0.1 5,945,626 - BT4527 0.35 -0.1 5,945,626 - BT4527 0.35 +0.1 5,945,739 + BT4527 0.56 -1.9 5,945,793 + BT4527 0.66 -0.2 5,945,793 + BT4527 0.66 -0.9 5,945,793 + BT4527 0.66 -0.7 5,945,794 - BT4527 0.66 +1.7 5,945,998 - -1.4 5,946,032 - +0.1 5,946,080 - BT4528 0.15 -0.3 5,946,092 + BT4528 0.17 -1.9 5,946,092 + BT4528 0.17 -1.9 5,946,169 + BT4528 0.30 -2.7 5,946,169 + BT4528 0.30 +0.1 5,946,186 + BT4528 0.33 -2.7 5,946,228 - BT4528 0.40 -1.4 5,946,228 - BT4528 0.40 -2.3 5,946,229 + BT4528 0.41 +2.1 5,946,230 - BT4528 0.41 +0.1 5,946,302 - BT4528 0.53 -3.3 5,946,304 - BT4528 0.53 -0.7 5,946,327 + BT4528 0.57 -1.4 5,946,436 - BT4528 0.76 -2.2 5,946,454 + BT4528 0.79 -1.1 5,946,456 + BT4528 0.79 -2.8 5,946,483 + BT4528 0.84 -2.0 5,946,541 - +0.1 5,946,553 - +0.7 5,946,568 - -2.2 5,946,749 - -4.3 5,946,749 - +0.1 5,946,756 + -1.0 5,946,756 + -4.3 5,946,757 - +0.7 5,946,769 - -0.9 5,946,875 + +2.1 5,946,876 - -2.2 5,946,991 + +2.7 5,946,992 - -2.4 5,946,996 + +0.1 5,946,998 + +1.1 5,946,999 - -0.9 5,947,034 + +1.7 5,947,098 + BT4529 0.30 +0.1 5,947,099 - BT4529 0.30 +2.5 5,947,101 - BT4529 0.31 +2.6 5,947,109 - BT4529 0.34 +1.5 5,947,113 - BT4529 0.35 +1.4 5,947,297 - +1.7 5,947,315 + -0.9 5,947,316 - +1.4 5,947,350 - +0.1 5,947,385 + -2.4 5,947,386 - -0.9 5,947,434 - +1.1 5,947,442 + BT4530 0.10 -0.4 5,947,548 + BT4530 0.18 +2.1 5,947,549 - BT4530 0.18 +1.5 5,947,560 - BT4530 0.19 +1.1
Or see this region's nucleotide sequence