Experiment: BHIS with Sulfamonomethoxine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1301 and BT1302 are separated by 224 nucleotides BT1302 and BT1303 are separated by 17 nucleotides BT1303 and BT1304 are separated by 14 nucleotides BT1304 and BT1305 are separated by 15 nucleotides
BT1301: BT1301 - ATP synthase subunit E (NCBI ptt file), at 1,622,032 to 1,622,622
BT1301
BT1302: BT1302 - hypothetical protein (NCBI ptt file), at 1,622,847 to 1,623,293
BT1302
BT1303: BT1303 - hypothetical protein (NCBI ptt file), at 1,623,311 to 1,624,111
BT1303
BT1304: BT1304 - putative transcriptional regulator (NCBI ptt file), at 1,624,126 to 1,624,497
BT1304
BT1305: BT1305 - hypothetical protein (NCBI ptt file), at 1,624,513 to 1,625,328
BT1305
Position (kb)
1623
1624
1625 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1622.647 kb on + strand at 1622.652 kb on + strand at 1622.760 kb on + strand at 1622.826 kb on - strand at 1622.870 kb on - strand at 1622.894 kb on + strand, within BT1302 at 1622.913 kb on - strand, within BT1302 at 1622.939 kb on + strand, within BT1302 at 1622.940 kb on - strand, within BT1302 at 1622.940 kb on - strand, within BT1302 at 1622.948 kb on - strand, within BT1302 at 1622.951 kb on + strand, within BT1302 at 1622.970 kb on - strand, within BT1302 at 1623.009 kb on - strand, within BT1302 at 1623.121 kb on - strand, within BT1302 at 1623.192 kb on - strand, within BT1302 at 1623.202 kb on - strand, within BT1302 at 1623.237 kb on - strand, within BT1302 at 1623.237 kb on - strand, within BT1302 at 1623.306 kb on + strand at 1623.324 kb on - strand at 1623.325 kb on + strand at 1623.383 kb on + strand at 1623.391 kb on + strand, within BT1303 at 1623.406 kb on + strand, within BT1303 at 1623.406 kb on + strand, within BT1303 at 1623.421 kb on + strand, within BT1303 at 1623.421 kb on + strand, within BT1303 at 1623.445 kb on - strand, within BT1303 at 1623.507 kb on + strand, within BT1303 at 1623.653 kb on - strand, within BT1303 at 1623.655 kb on + strand, within BT1303 at 1623.686 kb on + strand, within BT1303 at 1623.687 kb on - strand, within BT1303 at 1623.771 kb on + strand, within BT1303 at 1623.775 kb on + strand, within BT1303 at 1623.806 kb on + strand, within BT1303 at 1623.862 kb on + strand, within BT1303 at 1623.863 kb on - strand, within BT1303 at 1623.906 kb on - strand, within BT1303 at 1623.915 kb on - strand, within BT1303 at 1623.925 kb on + strand, within BT1303 at 1623.926 kb on - strand, within BT1303 at 1624.056 kb on - strand at 1624.091 kb on + strand at 1624.095 kb on + strand at 1624.112 kb on - strand at 1624.116 kb on + strand at 1624.144 kb on + strand at 1624.196 kb on + strand, within BT1304 at 1624.404 kb on + strand, within BT1304 at 1624.405 kb on - strand, within BT1304 at 1624.421 kb on + strand, within BT1304 at 1624.465 kb on + strand at 1624.488 kb on + strand at 1624.497 kb on + strand at 1624.501 kb on + strand at 1624.502 kb on - strand at 1624.641 kb on + strand, within BT1305 at 1624.666 kb on + strand, within BT1305 at 1624.696 kb on + strand, within BT1305 at 1624.709 kb on - strand, within BT1305 at 1624.816 kb on - strand, within BT1305 at 1624.857 kb on - strand, within BT1305 at 1624.877 kb on - strand, within BT1305 at 1624.878 kb on + strand, within BT1305 at 1624.879 kb on - strand, within BT1305 at 1624.898 kb on - strand, within BT1305 at 1624.937 kb on - strand, within BT1305 at 1624.954 kb on - strand, within BT1305 at 1624.958 kb on - strand, within BT1305 at 1624.968 kb on + strand, within BT1305 at 1624.972 kb on + strand, within BT1305 at 1624.973 kb on - strand, within BT1305 at 1624.977 kb on - strand, within BT1305 at 1624.977 kb on - strand, within BT1305 at 1625.013 kb on + strand, within BT1305 at 1625.014 kb on - strand, within BT1305 at 1625.021 kb on - strand, within BT1305 at 1625.021 kb on - strand, within BT1305 at 1625.021 kb on - strand, within BT1305 at 1625.079 kb on + strand, within BT1305
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfamonomethoxine 0.2 mM remove 1,622,647 + +2.7 1,622,652 + +0.1 1,622,760 + -1.5 1,622,826 - +0.6 1,622,870 - +2.7 1,622,894 + BT1302 0.11 -2.2 1,622,913 - BT1302 0.15 -1.5 1,622,939 + BT1302 0.21 +0.1 1,622,940 - BT1302 0.21 -0.9 1,622,940 - BT1302 0.21 -2.0 1,622,948 - BT1302 0.23 -2.3 1,622,951 + BT1302 0.23 +0.1 1,622,970 - BT1302 0.28 +0.1 1,623,009 - BT1302 0.36 -2.5 1,623,121 - BT1302 0.61 -0.6 1,623,192 - BT1302 0.77 +1.4 1,623,202 - BT1302 0.79 +0.8 1,623,237 - BT1302 0.87 -4.8 1,623,237 - BT1302 0.87 -2.1 1,623,306 + +0.1 1,623,324 - +3.1 1,623,325 + -2.9 1,623,383 + -2.9 1,623,391 + BT1303 0.10 -3.5 1,623,406 + BT1303 0.12 -1.2 1,623,406 + BT1303 0.12 -0.5 1,623,421 + BT1303 0.14 -2.9 1,623,421 + BT1303 0.14 +2.1 1,623,445 - BT1303 0.17 -1.1 1,623,507 + BT1303 0.24 -1.4 1,623,653 - BT1303 0.43 +0.1 1,623,655 + BT1303 0.43 -1.5 1,623,686 + BT1303 0.47 -0.5 1,623,687 - BT1303 0.47 +2.6 1,623,771 + BT1303 0.57 -2.0 1,623,775 + BT1303 0.58 +0.7 1,623,806 + BT1303 0.62 -0.5 1,623,862 + BT1303 0.69 -2.1 1,623,863 - BT1303 0.69 -2.4 1,623,906 - BT1303 0.74 -1.5 1,623,915 - BT1303 0.75 +0.1 1,623,925 + BT1303 0.77 +0.1 1,623,926 - BT1303 0.77 -1.1 1,624,056 - +0.1 1,624,091 + +1.1 1,624,095 + -2.4 1,624,112 - -2.8 1,624,116 + +0.1 1,624,144 + -1.9 1,624,196 + BT1304 0.19 +0.1 1,624,404 + BT1304 0.75 +0.1 1,624,405 - BT1304 0.75 -1.5 1,624,421 + BT1304 0.79 -1.6 1,624,465 + -2.8 1,624,488 + -2.2 1,624,497 + +0.1 1,624,501 + -1.2 1,624,502 - -3.1 1,624,641 + BT1305 0.16 -2.7 1,624,666 + BT1305 0.19 -0.3 1,624,696 + BT1305 0.22 -2.7 1,624,709 - BT1305 0.24 -2.9 1,624,816 - BT1305 0.37 +1.5 1,624,857 - BT1305 0.42 -1.8 1,624,877 - BT1305 0.45 +0.7 1,624,878 + BT1305 0.45 -0.5 1,624,879 - BT1305 0.45 +0.1 1,624,898 - BT1305 0.47 +0.1 1,624,937 - BT1305 0.52 +0.1 1,624,954 - BT1305 0.54 -1.5 1,624,958 - BT1305 0.55 +0.1 1,624,968 + BT1305 0.56 -0.5 1,624,972 + BT1305 0.56 +1.4 1,624,973 - BT1305 0.56 +0.1 1,624,977 - BT1305 0.57 -0.9 1,624,977 - BT1305 0.57 -0.9 1,625,013 + BT1305 0.61 +0.1 1,625,014 - BT1305 0.61 -1.2 1,625,021 - BT1305 0.62 +0.1 1,625,021 - BT1305 0.62 -0.9 1,625,021 - BT1305 0.62 -1.5 1,625,079 + BT1305 0.69 -1.5
Or see this region's nucleotide sequence