Experiment: KB with Avocadyne 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3269 and Psyr_3270 are separated by 42 nucleotides Psyr_3270 and Psyr_3271 are separated by 35 nucleotides
Psyr_3269: Psyr_3269 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, at 3,914,548 to 3,916,485
_3269
Psyr_3270: Psyr_3270 - AP endonuclease, family 2, at 3,916,528 to 3,917,358
_3270
Psyr_3271: Psyr_3271 - methylmalonate-semialdehyde dehydrogenase (acylating), at 3,917,394 to 3,918,896
_3271
Position (kb)
3916
3917
3918 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3915.811 kb on + strand, within Psyr_3269 at 3915.811 kb on + strand, within Psyr_3269 at 3915.811 kb on + strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.903 kb on - strand at 3916.031 kb on + strand, within Psyr_3269 at 3916.427 kb on + strand at 3916.428 kb on - strand at 3916.428 kb on - strand at 3916.479 kb on + strand at 3916.479 kb on + strand at 3916.479 kb on + strand at 3916.479 kb on + strand at 3916.479 kb on + strand at 3916.480 kb on - strand at 3916.480 kb on - strand at 3916.480 kb on - strand at 3916.480 kb on - strand at 3916.647 kb on - strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.774 kb on + strand, within Psyr_3270 at 3916.774 kb on + strand, within Psyr_3270 at 3916.774 kb on + strand, within Psyr_3270 at 3916.774 kb on + strand, within Psyr_3270 at 3916.774 kb on + strand, within Psyr_3270 at 3916.774 kb on + strand, within Psyr_3270 at 3916.774 kb on + strand, within Psyr_3270 at 3916.775 kb on - strand, within Psyr_3270 at 3916.775 kb on - strand, within Psyr_3270 at 3916.775 kb on - strand, within Psyr_3270 at 3916.775 kb on - strand, within Psyr_3270 at 3916.775 kb on - strand, within Psyr_3270 at 3917.171 kb on - strand, within Psyr_3270 at 3917.395 kb on + strand at 3917.395 kb on + strand at 3917.396 kb on - strand at 3917.396 kb on - strand at 3917.477 kb on - strand at 3917.752 kb on + strand, within Psyr_3271 at 3917.752 kb on + strand, within Psyr_3271 at 3917.752 kb on + strand, within Psyr_3271 at 3918.067 kb on + strand, within Psyr_3271 at 3918.068 kb on - strand, within Psyr_3271 at 3918.128 kb on + strand, within Psyr_3271 at 3918.128 kb on + strand, within Psyr_3271 at 3918.129 kb on - strand, within Psyr_3271 at 3918.129 kb on - strand, within Psyr_3271 at 3918.129 kb on - strand, within Psyr_3271 at 3918.129 kb on - strand, within Psyr_3271 at 3918.193 kb on + strand, within Psyr_3271 at 3918.193 kb on + strand, within Psyr_3271 at 3918.193 kb on + strand, within Psyr_3271 at 3918.194 kb on - strand, within Psyr_3271 at 3918.194 kb on - strand, within Psyr_3271 at 3918.194 kb on - strand, within Psyr_3271 at 3918.194 kb on - strand, within Psyr_3271 at 3918.194 kb on - strand, within Psyr_3271 at 3918.194 kb on - strand, within Psyr_3271
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadyne 10 mM remove 3,915,811 + Psyr_3269 0.65 +1.0 3,915,811 + Psyr_3269 0.65 -0.2 3,915,811 + Psyr_3269 0.65 +0.6 3,915,812 - Psyr_3269 0.65 -0.8 3,915,812 - Psyr_3269 0.65 +1.0 3,915,812 - Psyr_3269 0.65 +1.0 3,915,812 - Psyr_3269 0.65 +0.1 3,915,812 - Psyr_3269 0.65 +0.8 3,915,812 - Psyr_3269 0.65 +0.1 3,915,812 - Psyr_3269 0.65 -1.2 3,915,903 - +2.6 3,916,031 + Psyr_3269 0.77 +0.7 3,916,427 + +2.0 3,916,428 - +0.6 3,916,428 - -0.0 3,916,479 + -0.4 3,916,479 + +1.4 3,916,479 + -0.9 3,916,479 + +0.1 3,916,479 + -0.9 3,916,480 - -1.9 3,916,480 - -1.9 3,916,480 - -0.1 3,916,480 - -0.8 3,916,647 - Psyr_3270 0.14 -0.6 3,916,770 + Psyr_3270 0.29 -0.6 3,916,770 + Psyr_3270 0.29 +2.6 3,916,770 + Psyr_3270 0.29 -0.1 3,916,770 + Psyr_3270 0.29 -0.0 3,916,770 + Psyr_3270 0.29 +1.1 3,916,770 + Psyr_3270 0.29 -0.9 3,916,770 + Psyr_3270 0.29 -0.6 3,916,770 + Psyr_3270 0.29 +0.1 3,916,770 + Psyr_3270 0.29 -2.6 3,916,771 - Psyr_3270 0.29 +1.3 3,916,771 - Psyr_3270 0.29 +2.6 3,916,771 - Psyr_3270 0.29 +2.8 3,916,771 - Psyr_3270 0.29 +1.8 3,916,771 - Psyr_3270 0.29 +1.8 3,916,771 - Psyr_3270 0.29 -1.6 3,916,771 - Psyr_3270 0.29 +0.4 3,916,771 - Psyr_3270 0.29 +1.0 3,916,771 - Psyr_3270 0.29 -0.6 3,916,774 + Psyr_3270 0.30 +0.8 3,916,774 + Psyr_3270 0.30 +1.4 3,916,774 + Psyr_3270 0.30 +0.0 3,916,774 + Psyr_3270 0.30 +0.9 3,916,774 + Psyr_3270 0.30 -0.1 3,916,774 + Psyr_3270 0.30 +0.3 3,916,774 + Psyr_3270 0.30 -0.2 3,916,775 - Psyr_3270 0.30 +1.7 3,916,775 - Psyr_3270 0.30 +0.3 3,916,775 - Psyr_3270 0.30 +2.1 3,916,775 - Psyr_3270 0.30 +1.5 3,916,775 - Psyr_3270 0.30 +0.8 3,917,171 - Psyr_3270 0.77 +0.2 3,917,395 + +0.4 3,917,395 + -1.2 3,917,396 - -2.9 3,917,396 - +0.7 3,917,477 - +0.6 3,917,752 + Psyr_3271 0.24 -2.3 3,917,752 + Psyr_3271 0.24 +0.1 3,917,752 + Psyr_3271 0.24 +0.7 3,918,067 + Psyr_3271 0.45 +0.7 3,918,068 - Psyr_3271 0.45 -1.8 3,918,128 + Psyr_3271 0.49 -1.6 3,918,128 + Psyr_3271 0.49 -0.6 3,918,129 - Psyr_3271 0.49 +0.4 3,918,129 - Psyr_3271 0.49 -1.9 3,918,129 - Psyr_3271 0.49 +0.2 3,918,129 - Psyr_3271 0.49 -0.8 3,918,193 + Psyr_3271 0.53 -0.9 3,918,193 + Psyr_3271 0.53 +1.2 3,918,193 + Psyr_3271 0.53 -0.2 3,918,194 - Psyr_3271 0.53 -0.8 3,918,194 - Psyr_3271 0.53 +1.2 3,918,194 - Psyr_3271 0.53 +0.1 3,918,194 - Psyr_3271 0.53 -0.5 3,918,194 - Psyr_3271 0.53 +1.0 3,918,194 - Psyr_3271 0.53 -1.2
Or see this region's nucleotide sequence