Experiment: KB with Avocadyne 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1811 and Psyr_1812 are separated by 491 nucleotides Psyr_1812 and Psyr_1813 overlap by 4 nucleotides Psyr_1813 and Psyr_1814 overlap by 4 nucleotides
Psyr_1811: Psyr_1811 - transcriptional regulator, GntR family, at 2,083,963 to 2,084,712
_1811
Psyr_1812: Psyr_1812 - guanine deaminase, at 2,085,204 to 2,086,538
_1812
Psyr_1813: Psyr_1813 - molybdenum cofactor sulfurylase, at 2,086,535 to 2,087,374
_1813
Psyr_1814: Psyr_1814 - xanthine dehydrogenase, molybdenum binding subunit apoprotein, at 2,087,371 to 2,089,890
_1814
Position (kb)
2085
2086
2087 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2084.530 kb on + strand, within Psyr_1811 at 2084.530 kb on + strand, within Psyr_1811 at 2084.531 kb on - strand, within Psyr_1811 at 2084.943 kb on + strand at 2084.943 kb on + strand at 2084.944 kb on - strand at 2084.979 kb on - strand at 2085.206 kb on - strand at 2085.300 kb on - strand at 2085.442 kb on + strand, within Psyr_1812 at 2085.442 kb on + strand, within Psyr_1812 at 2085.443 kb on - strand, within Psyr_1812 at 2085.493 kb on + strand, within Psyr_1812 at 2085.493 kb on + strand, within Psyr_1812 at 2085.493 kb on + strand, within Psyr_1812 at 2085.721 kb on - strand, within Psyr_1812 at 2085.751 kb on + strand, within Psyr_1812 at 2085.826 kb on + strand, within Psyr_1812 at 2085.826 kb on + strand, within Psyr_1812 at 2085.826 kb on + strand, within Psyr_1812 at 2085.826 kb on + strand, within Psyr_1812 at 2085.913 kb on + strand, within Psyr_1812 at 2086.210 kb on + strand, within Psyr_1812 at 2086.210 kb on + strand, within Psyr_1812 at 2086.211 kb on - strand, within Psyr_1812 at 2086.211 kb on - strand, within Psyr_1812 at 2086.211 kb on - strand, within Psyr_1812 at 2086.244 kb on - strand, within Psyr_1812 at 2086.319 kb on - strand, within Psyr_1812 at 2086.423 kb on + strand at 2086.424 kb on - strand at 2086.424 kb on - strand at 2086.424 kb on - strand at 2086.424 kb on - strand at 2086.620 kb on + strand, within Psyr_1813 at 2086.620 kb on + strand, within Psyr_1813 at 2086.620 kb on + strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.621 kb on - strand, within Psyr_1813 at 2086.797 kb on + strand, within Psyr_1813 at 2086.875 kb on + strand, within Psyr_1813 at 2086.875 kb on + strand, within Psyr_1813 at 2086.875 kb on + strand, within Psyr_1813 at 2086.875 kb on + strand, within Psyr_1813 at 2086.875 kb on + strand, within Psyr_1813 at 2086.876 kb on - strand, within Psyr_1813 at 2086.876 kb on - strand, within Psyr_1813 at 2086.876 kb on - strand, within Psyr_1813 at 2086.876 kb on - strand, within Psyr_1813 at 2087.208 kb on + strand, within Psyr_1813 at 2087.208 kb on + strand, within Psyr_1813 at 2087.209 kb on - strand, within Psyr_1813 at 2087.278 kb on + strand, within Psyr_1813 at 2087.279 kb on - strand, within Psyr_1813 at 2087.448 kb on + strand at 2087.449 kb on - strand at 2087.449 kb on - strand at 2087.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadyne 10 mM remove 2,084,530 + Psyr_1811 0.76 -0.7 2,084,530 + Psyr_1811 0.76 +0.2 2,084,531 - Psyr_1811 0.76 +0.9 2,084,943 + -0.1 2,084,943 + +1.3 2,084,944 - -0.8 2,084,979 - -0.6 2,085,206 - +1.0 2,085,300 - +0.3 2,085,442 + Psyr_1812 0.18 -1.9 2,085,442 + Psyr_1812 0.18 +1.0 2,085,443 - Psyr_1812 0.18 +1.3 2,085,493 + Psyr_1812 0.22 +4.7 2,085,493 + Psyr_1812 0.22 -0.9 2,085,493 + Psyr_1812 0.22 -0.8 2,085,721 - Psyr_1812 0.39 -0.0 2,085,751 + Psyr_1812 0.41 -2.0 2,085,826 + Psyr_1812 0.47 -1.5 2,085,826 + Psyr_1812 0.47 -0.2 2,085,826 + Psyr_1812 0.47 -1.1 2,085,826 + Psyr_1812 0.47 -2.0 2,085,913 + Psyr_1812 0.53 +1.6 2,086,210 + Psyr_1812 0.75 -1.6 2,086,210 + Psyr_1812 0.75 +1.4 2,086,211 - Psyr_1812 0.75 -1.1 2,086,211 - Psyr_1812 0.75 -0.8 2,086,211 - Psyr_1812 0.75 +1.0 2,086,244 - Psyr_1812 0.78 -1.5 2,086,319 - Psyr_1812 0.84 +1.5 2,086,423 + +2.3 2,086,424 - +3.0 2,086,424 - -0.0 2,086,424 - +1.6 2,086,424 - -1.4 2,086,620 + Psyr_1813 0.10 +2.6 2,086,620 + Psyr_1813 0.10 -0.2 2,086,620 + Psyr_1813 0.10 +1.6 2,086,621 - Psyr_1813 0.10 +2.0 2,086,621 - Psyr_1813 0.10 -0.7 2,086,621 - Psyr_1813 0.10 -1.1 2,086,621 - Psyr_1813 0.10 +0.6 2,086,621 - Psyr_1813 0.10 +0.5 2,086,621 - Psyr_1813 0.10 -0.1 2,086,621 - Psyr_1813 0.10 +0.5 2,086,621 - Psyr_1813 0.10 +1.0 2,086,621 - Psyr_1813 0.10 +0.6 2,086,621 - Psyr_1813 0.10 +0.8 2,086,797 + Psyr_1813 0.31 -1.1 2,086,875 + Psyr_1813 0.40 +1.0 2,086,875 + Psyr_1813 0.40 -0.3 2,086,875 + Psyr_1813 0.40 +0.2 2,086,875 + Psyr_1813 0.40 +0.5 2,086,875 + Psyr_1813 0.40 +0.2 2,086,876 - Psyr_1813 0.41 -0.5 2,086,876 - Psyr_1813 0.41 +2.2 2,086,876 - Psyr_1813 0.41 -0.8 2,086,876 - Psyr_1813 0.41 -0.1 2,087,208 + Psyr_1813 0.80 -0.2 2,087,208 + Psyr_1813 0.80 +0.6 2,087,209 - Psyr_1813 0.80 -0.0 2,087,278 + Psyr_1813 0.88 +1.0 2,087,279 - Psyr_1813 0.89 -1.1 2,087,448 + -0.8 2,087,449 - +0.5 2,087,449 - -1.0 2,087,449 - +0.5
Or see this region's nucleotide sequence