Experiment: KB with Avocadyne 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0131 and Psyr_0132 are separated by 100 nucleotides Psyr_0132 and Psyr_0133 are separated by 83 nucleotides Psyr_0133 and Psyr_0134 are separated by 851 nucleotides
Psyr_0131: Psyr_0131 - HAD-superfamily hydrolase, subfamily IA, variant 3:HAD-superfamily hydrolase, subfamily IA, variant 1, at 139,587 to 140,261
_0131
Psyr_0132: Psyr_0132 - conserved hypothetical protein, at 140,362 to 140,610
_0132
Psyr_0133: Psyr_0133 - transcriptional regulator, AsnC family, at 140,694 to 141,164
_0133
Psyr_0134: Psyr_0134 - Aminotransferase, class V, at 142,016 to 143,203
_0134
Position (kb)
140
141
142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 139.710 kb on - strand, within Psyr_0131 at 139.710 kb on - strand, within Psyr_0131 at 139.827 kb on + strand at 139.828 kb on - strand, within Psyr_0131 at 140.014 kb on - strand, within Psyr_0131 at 140.014 kb on - strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.060 kb on - strand, within Psyr_0131 at 140.205 kb on + strand at 140.205 kb on + strand at 140.205 kb on + strand at 140.205 kb on + strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.265 kb on + strand at 140.305 kb on + strand at 140.305 kb on + strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.356 kb on - strand at 140.356 kb on - strand at 140.356 kb on - strand at 140.435 kb on + strand, within Psyr_0132 at 140.435 kb on + strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.540 kb on + strand, within Psyr_0132 at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.713 kb on + strand at 140.713 kb on + strand at 140.714 kb on - strand at 140.714 kb on - strand at 140.806 kb on + strand, within Psyr_0133 at 140.807 kb on - strand, within Psyr_0133 at 140.807 kb on - strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.907 kb on + strand, within Psyr_0133 at 140.908 kb on - strand, within Psyr_0133 at 141.071 kb on + strand, within Psyr_0133 at 141.190 kb on + strand at 141.190 kb on + strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.561 kb on - strand at 141.561 kb on - strand at 141.813 kb on + strand at 141.813 kb on + strand at 141.813 kb on + strand at 141.813 kb on + strand at 141.814 kb on - strand at 141.814 kb on - strand at 141.814 kb on - strand at 141.814 kb on - strand at 141.826 kb on + strand at 141.881 kb on - strand at 141.948 kb on + strand at 141.949 kb on - strand at 141.952 kb on + strand at 141.952 kb on + strand at 141.953 kb on - strand at 141.980 kb on + strand at 141.981 kb on - strand at 141.981 kb on - strand at 142.033 kb on - strand at 142.033 kb on - strand at 142.067 kb on + strand at 142.067 kb on + strand at 142.076 kb on + strand at 142.077 kb on - strand at 142.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadyne 10 mM remove 139,710 - Psyr_0131 0.18 +0.1 139,710 - Psyr_0131 0.18 +2.0 139,827 + +1.4 139,828 - Psyr_0131 0.36 -1.2 140,014 - Psyr_0131 0.63 -0.2 140,014 - Psyr_0131 0.63 -0.5 140,059 + Psyr_0131 0.70 -1.0 140,059 + Psyr_0131 0.70 +0.7 140,059 + Psyr_0131 0.70 +0.7 140,059 + Psyr_0131 0.70 +0.5 140,059 + Psyr_0131 0.70 +1.6 140,059 + Psyr_0131 0.70 +0.8 140,060 - Psyr_0131 0.70 +2.0 140,205 + -2.0 140,205 + -0.2 140,205 + +1.4 140,205 + +0.6 140,206 - -0.1 140,206 - -0.1 140,206 - -0.6 140,206 - +0.3 140,206 - +2.0 140,206 - -0.7 140,206 - +0.0 140,206 - +1.1 140,206 - +0.0 140,206 - +0.2 140,265 + -0.7 140,305 + +0.5 140,305 + +0.4 140,306 - +0.1 140,306 - -0.4 140,306 - +0.2 140,306 - +0.2 140,306 - -0.9 140,306 - -1.4 140,355 + +0.6 140,355 + +0.1 140,355 + -0.3 140,355 + -0.5 140,355 + +1.0 140,356 - +1.9 140,356 - +2.9 140,356 - -0.0 140,435 + Psyr_0132 0.29 -0.6 140,435 + Psyr_0132 0.29 -0.6 140,436 - Psyr_0132 0.30 -0.6 140,436 - Psyr_0132 0.30 +0.1 140,436 - Psyr_0132 0.30 +0.4 140,436 - Psyr_0132 0.30 -0.0 140,436 - Psyr_0132 0.30 -0.3 140,540 + Psyr_0132 0.71 +0.7 140,593 + +0.4 140,593 + -1.4 140,593 + +1.3 140,593 + -0.1 140,593 + -0.8 140,593 + -0.9 140,593 + -0.2 140,593 + +0.2 140,593 + +0.1 140,593 + -0.8 140,593 + +0.2 140,594 - +0.1 140,594 - +0.3 140,594 - -0.0 140,594 - -1.7 140,594 - -1.4 140,713 + +1.2 140,713 + +0.2 140,714 - +0.1 140,714 - -0.4 140,806 + Psyr_0133 0.24 -1.1 140,807 - Psyr_0133 0.24 +2.0 140,807 - Psyr_0133 0.24 -0.1 140,854 + Psyr_0133 0.34 -0.9 140,854 + Psyr_0133 0.34 +0.6 140,854 + Psyr_0133 0.34 -0.5 140,854 + Psyr_0133 0.34 -0.3 140,855 - Psyr_0133 0.34 -2.3 140,855 - Psyr_0133 0.34 -1.0 140,855 - Psyr_0133 0.34 +0.5 140,855 - Psyr_0133 0.34 -1.0 140,855 - Psyr_0133 0.34 -0.9 140,855 - Psyr_0133 0.34 -0.7 140,855 - Psyr_0133 0.34 -0.2 140,907 + Psyr_0133 0.45 -0.3 140,908 - Psyr_0133 0.45 -0.2 141,071 + Psyr_0133 0.80 -1.0 141,190 + +0.4 141,190 + -1.0 141,191 - -1.4 141,191 - -0.4 141,191 - -0.9 141,191 - +1.0 141,191 - +0.4 141,191 - -0.8 141,191 - -1.1 141,191 - -0.8 141,233 - +0.3 141,233 - -0.2 141,233 - +1.1 141,233 - +1.0 141,233 - -0.1 141,561 - +0.2 141,561 - -0.7 141,813 + +0.7 141,813 + -0.9 141,813 + +0.1 141,813 + -1.4 141,814 - -3.3 141,814 - -0.4 141,814 - +0.1 141,814 - +1.3 141,826 + -0.6 141,881 - +0.7 141,948 + -0.7 141,949 - -0.0 141,952 + +0.9 141,952 + +0.4 141,953 - -0.7 141,980 + -0.5 141,981 - -1.6 141,981 - +0.7 142,033 - -0.5 142,033 - +0.2 142,067 + -0.2 142,067 + +1.0 142,076 + +0.1 142,077 - +0.2 142,077 - +0.3
Or see this region's nucleotide sequence