Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23240 and BWI76_RS23245 are separated by 12 nucleotides BWI76_RS23245 and BWI76_RS23250 overlap by 16 nucleotides BWI76_RS23250 and BWI76_RS23255 overlap by 4 nucleotides BWI76_RS23255 and BWI76_RS23260 overlap by 10 nucleotides BWI76_RS23260 and BWI76_RS23265 are separated by 218 nucleotides
BWI76_RS23240: BWI76_RS23240 - exonuclease V subunit gamma, at 4,846,841 to 4,850,215
_RS23240
BWI76_RS23245: BWI76_RS23245 - hypothetical protein, at 4,850,228 to 4,850,551
_RS23245
BWI76_RS23250: BWI76_RS23250 - hypothetical protein, at 4,850,536 to 4,850,943
_RS23250
BWI76_RS23255: BWI76_RS23255 - prepilin-type cleavage/methylation domain-containing protein, at 4,850,940 to 4,851,503
_RS23255
BWI76_RS23260: BWI76_RS23260 - prepilin-type cleavage/methylation domain-containing protein, at 4,851,494 to 4,851,961
_RS23260
BWI76_RS23265: BWI76_RS23265 - hypothetical protein, at 4,852,180 to 4,853,856
_RS23265
Position (kb) 4850
4851
4852 Strain fitness (log2 ratio) -1
0
1 at 4850.375 kb on - strand, within BWI76_RS23245 at 4850.433 kb on + strand, within BWI76_RS23245 at 4850.679 kb on - strand, within BWI76_RS23250 at 4850.784 kb on - strand, within BWI76_RS23250 at 4850.953 kb on - strand at 4851.173 kb on + strand, within BWI76_RS23255 at 4851.397 kb on - strand, within BWI76_RS23255 at 4851.397 kb on - strand, within BWI76_RS23255 at 4851.399 kb on + strand, within BWI76_RS23255 at 4851.499 kb on + strand at 4851.617 kb on - strand, within BWI76_RS23260 at 4851.617 kb on - strand at 4851.642 kb on - strand, within BWI76_RS23260 at 4851.803 kb on + strand, within BWI76_RS23260 at 4851.945 kb on - strand at 4852.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF remove 4,850,375 - BWI76_RS23245 0.45 +1.0 4,850,433 + BWI76_RS23245 0.63 +0.1 4,850,679 - BWI76_RS23250 0.35 -0.0 4,850,784 - BWI76_RS23250 0.61 +0.4 4,850,953 - +0.3 4,851,173 + BWI76_RS23255 0.41 +0.9 4,851,397 - BWI76_RS23255 0.81 +0.2 4,851,397 - BWI76_RS23255 0.81 +1.2 4,851,399 + BWI76_RS23255 0.81 +0.2 4,851,499 + -1.5 4,851,617 - BWI76_RS23260 0.26 -0.0 4,851,617 - +1.4 4,851,642 - BWI76_RS23260 0.32 +0.8 4,851,803 + BWI76_RS23260 0.66 +1.0 4,851,945 - +0.4 4,852,040 - +0.7
Or see this region's nucleotide sequence