Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22810

Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
4,756,100	+		BWI76_RS22805	0.60	-1.0
4,756,264	+		BWI76_RS22805	0.68	-1.7
4,756,303	+		BWI76_RS22805	0.69	-0.6
4,756,410	-		BWI76_RS22805	0.74	-1.1
4,756,531	+		BWI76_RS22805	0.80	-1.1
4,756,598	+		BWI76_RS22805	0.83	-1.8
4,756,598	+		BWI76_RS22805	0.83	-0.4
4,756,606	-		BWI76_RS22805	0.83	-0.7
4,756,661	-		BWI76_RS22805	0.85	+0.1
4,756,663	+		BWI76_RS22805	0.85	-0.4
4,756,746	-		BWI76_RS22805	0.89	-0.8
4,756,775	-				-0.7
4,756,997	+				+0.4
4,756,997	+				-1.1
4,756,997	+				-0.2
4,757,003	-				+0.1
4,757,005	-				+0.0
4,757,005	-				+0.8
4,757,005	-				-0.9
4,757,005	-				-0.1
4,757,042	+				-1.4
4,757,042	-				+0.3
4,757,531	+		BWI76_RS22810	0.33	-1.4
4,757,637	+		BWI76_RS22810	0.41	-0.9
4,757,673	+		BWI76_RS22810	0.44	-1.6
4,757,689	+		BWI76_RS22810	0.45	-1.0
4,757,945	-		BWI76_RS22810	0.64	-0.4
4,758,076	-		BWI76_RS22810	0.74	-0.0
4,758,227	+		BWI76_RS22810	0.85	-0.8
4,758,227	+		BWI76_RS22810	0.85	+0.8
4,758,227	+		BWI76_RS22810	0.85	-0.3
4,758,235	-		BWI76_RS22810	0.85	-1.0
4,758,377	+				-1.4
4,758,377	+				-0.8
4,758,444	-				+0.5
4,758,490	-				+0.7
4,758,490	-				-0.2
4,758,520	-				+0.5
4,758,561	+				+0.2
4,758,569	-				+0.8
4,758,608	+				-0.0
4,758,672	+				-0.2
4,758,704	-				+0.5
4,758,891	-		BWI76_RS22815	0.12	+0.7
4,758,959	-		BWI76_RS22815	0.15	+0.3
4,759,116	-		BWI76_RS22815	0.20	+0.3
4,759,214	+		BWI76_RS22815	0.24	+0.6
4,759,234	-		BWI76_RS22815	0.25	+1.0