Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21200

Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21195 and BWI76_RS21200 are separated by 76 nucleotidesBWI76_RS21200 and BWI76_RS21205 overlap by 1 nucleotides BWI76_RS21195: BWI76_RS21195 - 3-phenylpropionic acid transporter, at 4,436,891 to 4,438,024 _RS21195 BWI76_RS21200: BWI76_RS21200 - aldose epimerase, at 4,438,101 to 4,438,937 _RS21200 BWI76_RS21205: BWI76_RS21205 - MFS transporter, at 4,438,937 to 4,440,373 _RS21205 Position (kb) 4438 4439Strain fitness (log2 ratio) -2 -1 0 1at 4437.147 kb on + strand, within BWI76_RS21195at 4437.434 kb on - strand, within BWI76_RS21195at 4437.522 kb on - strand, within BWI76_RS21195at 4437.539 kb on - strand, within BWI76_RS21195at 4437.577 kb on + strand, within BWI76_RS21195at 4437.779 kb on + strand, within BWI76_RS21195at 4437.927 kb on - strandat 4437.951 kb on + strandat 4437.963 kb on - strandat 4438.392 kb on + strand, within BWI76_RS21200at 4438.461 kb on + strand, within BWI76_RS21200at 4438.526 kb on - strand, within BWI76_RS21200at 4438.526 kb on - strand, within BWI76_RS21200at 4438.549 kb on - strand, within BWI76_RS21200at 4438.767 kb on + strand, within BWI76_RS21200at 4438.979 kb on + strandat 4439.107 kb on + strand, within BWI76_RS21205at 4439.110 kb on - strand, within BWI76_RS21205at 4439.247 kb on - strand, within BWI76_RS21205at 4439.282 kb on + strand, within BWI76_RS21205at 4439.373 kb on + strand, within BWI76_RS21205at 4439.405 kb on - strand, within BWI76_RS21205at 4439.577 kb on + strand, within BWI76_RS21205at 4439.708 kb on - strand, within BWI76_RS21205at 4439.923 kb on + strand, within BWI76_RS21205

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
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4,437,147 + BWI76_RS21195 0.23 -0.3
4,437,434 - BWI76_RS21195 0.48 +0.5
4,437,522 - BWI76_RS21195 0.56 -0.5
4,437,539 - BWI76_RS21195 0.57 -0.1
4,437,577 + BWI76_RS21195 0.60 +1.0
4,437,779 + BWI76_RS21195 0.78 +0.2
4,437,927 - +1.0
4,437,951 + +0.3
4,437,963 - -0.1
4,438,392 + BWI76_RS21200 0.35 -0.9
4,438,461 + BWI76_RS21200 0.43 -1.1
4,438,526 - BWI76_RS21200 0.51 -1.0
4,438,526 - BWI76_RS21200 0.51 +1.0
4,438,549 - BWI76_RS21200 0.54 -2.2
4,438,767 + BWI76_RS21200 0.80 -0.9
4,438,979 + -0.3
4,439,107 + BWI76_RS21205 0.12 -0.0
4,439,110 - BWI76_RS21205 0.12 +0.3
4,439,247 - BWI76_RS21205 0.22 +0.1
4,439,282 + BWI76_RS21205 0.24 +0.0
4,439,373 + BWI76_RS21205 0.30 -0.6
4,439,405 - BWI76_RS21205 0.33 -0.0
4,439,577 + BWI76_RS21205 0.45 -0.1
4,439,708 - BWI76_RS21205 0.54 -0.3
4,439,923 + BWI76_RS21205 0.69 +0.8

Or see this region's nucleotide sequence