Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16460 and BWI76_RS16465 are separated by 48 nucleotides BWI76_RS16465 and BWI76_RS16470 are separated by 142 nucleotides BWI76_RS16470 and BWI76_RS16475 are separated by 53 nucleotides
BWI76_RS16460: BWI76_RS16460 - oxidoreductase, at 3,414,116 to 3,415,012
_RS16460
BWI76_RS16465: BWI76_RS16465 - DUF1289 domain-containing protein, at 3,415,061 to 3,415,300
_RS16465
BWI76_RS16470: BWI76_RS16470 - transcriptional repressor NemR, at 3,415,443 to 3,416,042
_RS16470
BWI76_RS16475: BWI76_RS16475 - alkene reductase, at 3,416,096 to 3,417,193
_RS16475
Position (kb)
3415
3416
3417 Strain fitness (log2 ratio)
-1
0
1 at 3414.814 kb on - strand, within BWI76_RS16460 at 3414.983 kb on + strand at 3415.448 kb on + strand at 3415.499 kb on + strand at 3415.619 kb on + strand, within BWI76_RS16470 at 3416.082 kb on + strand at 3416.187 kb on + strand at 3416.195 kb on - strand at 3416.213 kb on - strand, within BWI76_RS16475 at 3416.290 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.333 kb on - strand, within BWI76_RS16475 at 3416.717 kb on + strand, within BWI76_RS16475 at 3416.725 kb on - strand, within BWI76_RS16475 at 3417.036 kb on + strand, within BWI76_RS16475
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF remove 3,414,814 - BWI76_RS16460 0.78 +0.2 3,414,983 + -0.2 3,415,448 + +0.4 3,415,499 + +0.4 3,415,619 + BWI76_RS16470 0.29 -0.7 3,416,082 + -0.7 3,416,187 + -0.1 3,416,195 - +0.0 3,416,213 - BWI76_RS16475 0.11 -0.5 3,416,290 + BWI76_RS16475 0.18 +0.0 3,416,325 + BWI76_RS16475 0.21 -0.3 3,416,325 + BWI76_RS16475 0.21 +0.6 3,416,333 - BWI76_RS16475 0.22 -0.1 3,416,717 + BWI76_RS16475 0.57 -0.2 3,416,725 - BWI76_RS16475 0.57 +0.5 3,417,036 + BWI76_RS16475 0.86 -0.0
Or see this region's nucleotide sequence