Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15930 and BWI76_RS15935 are separated by 1 nucleotides BWI76_RS15935 and BWI76_RS15940 are separated by 41 nucleotides BWI76_RS15940 and BWI76_RS15945 are separated by 139 nucleotides
BWI76_RS15930: BWI76_RS15930 - dimethylsulfoxide reductase, chain B, at 3,304,705 to 3,305,322
_RS15930
BWI76_RS15935: BWI76_RS15935 - dimethyl sulfoxide reductase, at 3,305,324 to 3,306,181
_RS15935
BWI76_RS15940: BWI76_RS15940 - DMSO reductase maturation protein DsmD, at 3,306,223 to 3,306,831
_RS15940
BWI76_RS15945: BWI76_RS15945 - voltage-gated ClC-type chloride channel ClcB, at 3,306,971 to 3,308,266
_RS15945
Position (kb)
3306
3307 Strain fitness (log2 ratio)
-1
0
1 at 3305.723 kb on + strand, within BWI76_RS15935 at 3306.388 kb on + strand, within BWI76_RS15940 at 3306.753 kb on - strand, within BWI76_RS15940 at 3306.795 kb on + strand at 3306.809 kb on + strand at 3306.832 kb on + strand at 3307.119 kb on + strand, within BWI76_RS15945 at 3307.760 kb on - strand, within BWI76_RS15945
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF remove 3,305,723 + BWI76_RS15935 0.47 +0.5 3,306,388 + BWI76_RS15940 0.27 -1.1 3,306,753 - BWI76_RS15940 0.87 -0.3 3,306,795 + -0.6 3,306,809 + -0.1 3,306,832 + +0.0 3,307,119 + BWI76_RS15945 0.11 -0.1 3,307,760 - BWI76_RS15945 0.61 -0.1
Or see this region's nucleotide sequence