Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11360 and BWI76_RS11365 are separated by 36 nucleotides BWI76_RS11365 and BWI76_RS11370 are separated by 0 nucleotides BWI76_RS11370 and BWI76_RS11375 overlap by 4 nucleotides
BWI76_RS11360: BWI76_RS11360 - L-asparaginase, at 2,394,301 to 2,395,344
_RS11360
BWI76_RS11365: BWI76_RS11365 - iron ABC transporter, at 2,395,381 to 2,396,148
_RS11365
BWI76_RS11370: BWI76_RS11370 - iron-dicitrate transporter subunit FecD, at 2,396,149 to 2,397,102
_RS11370
BWI76_RS11375: BWI76_RS11375 - iron-dicitrate transporter permease subunit, at 2,397,099 to 2,398,097
_RS11375
Position (kb)
2395
2396
2397 Strain fitness (log2 ratio)
-1
0
1 at 2394.477 kb on + strand, within BWI76_RS11360 at 2394.477 kb on + strand, within BWI76_RS11360 at 2394.485 kb on - strand, within BWI76_RS11360 at 2394.498 kb on + strand, within BWI76_RS11360 at 2394.598 kb on - strand, within BWI76_RS11360 at 2395.329 kb on + strand at 2395.541 kb on + strand, within BWI76_RS11365 at 2395.551 kb on + strand, within BWI76_RS11365 at 2395.784 kb on - strand, within BWI76_RS11365 at 2396.128 kb on - strand at 2396.177 kb on + strand at 2396.210 kb on + strand at 2396.870 kb on + strand, within BWI76_RS11370 at 2397.009 kb on + strand at 2397.136 kb on + strand at 2397.144 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF remove 2,394,477 + BWI76_RS11360 0.17 -0.7 2,394,477 + BWI76_RS11360 0.17 -0.3 2,394,485 - BWI76_RS11360 0.18 -0.1 2,394,498 + BWI76_RS11360 0.19 +0.4 2,394,598 - BWI76_RS11360 0.28 +0.1 2,395,329 + +0.4 2,395,541 + BWI76_RS11365 0.21 -0.8 2,395,551 + BWI76_RS11365 0.22 -0.4 2,395,784 - BWI76_RS11365 0.52 -0.0 2,396,128 - +0.7 2,396,177 + +0.3 2,396,210 + -0.2 2,396,870 + BWI76_RS11370 0.76 -0.1 2,397,009 + -0.1 2,397,136 + +0.7 2,397,144 - +0.0
Or see this region's nucleotide sequence