Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04505 and BWI76_RS04510 are separated by 40 nucleotides BWI76_RS04510 and BWI76_RS04515 are separated by 4 nucleotides BWI76_RS04515 and BWI76_RS04520 are separated by 2 nucleotides BWI76_RS04520 and BWI76_RS04525 overlap by 4 nucleotides
BWI76_RS04505: BWI76_RS04505 - dihydrofolate reductase, at 932,871 to 933,350
_RS04505
BWI76_RS04510: BWI76_RS04510 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 933,391 to 934,239
_RS04510
BWI76_RS04515: BWI76_RS04515 - Co2+/Mg2+ efflux protein ApaG, at 934,244 to 934,621
_RS04515
BWI76_RS04520: BWI76_RS04520 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase, at 934,624 to 935,445
_RS04520
BWI76_RS04525: BWI76_RS04525 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 935,442 to 936,431
_RS04525
Position (kb) 934
935 Strain fitness (log2 ratio) -1
0
1 at 933.478 kb on + strand, within BWI76_RS04510 at 933.624 kb on + strand, within BWI76_RS04510 at 934.061 kb on - strand, within BWI76_RS04510 at 934.344 kb on - strand, within BWI76_RS04515 at 934.605 kb on - strand at 935.290 kb on + strand, within BWI76_RS04520 at 935.422 kb on + strand at 935.511 kb on + strand at 935.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF remove 933,478 + BWI76_RS04510 0.10 -0.6 933,624 + BWI76_RS04510 0.27 -1.1 934,061 - BWI76_RS04510 0.79 -0.8 934,344 - BWI76_RS04515 0.26 -0.7 934,605 - +0.1 935,290 + BWI76_RS04520 0.81 +0.2 935,422 + -0.0 935,511 + +0.4 935,533 - +0.3
Or see this region's nucleotide sequence